The present study aimed to assess the antimicrobial resistance and the presence of virulence markers in 137 Listeria monocytogenes isolates obtained from meat-processing environments, beef products, and clinical cases. All isolates were subject to molecular serogrouping and their antibiotic resistance profiles were assessed against 12 antimicrobials. In addition, isolates were subjected to detection of virulence marker genes (inlA, inlC, inlJ). The isolates were classified into serogroups 4b, 4d, 4a, or 4c (46%), 1/2c or 3c (27%), 1/2a or 3a (13.9%), and 1/2b or 3b (13.1%). All tested isolates presented sensitivity to the majority of the tested antimicrobials, but most of them presented resistance or intermediate resistance to clindamycin (88.3%) and oxacillin (73.7%). Virulence markers were detected in all isolates, demanding further analysis to better characterize their pathogenic potential.
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References
1.
AllenK.J., Wałecka-ZacharskaE., ChenJ.C., Kosek-PaszkowskaK., DevlieghereF., Van MeervenneE., OsekJ., WieczorekK., and BaniaJ.2014. Listeria monocytogenes—an examination of food chain factors potentially contributing to antimicrobial resistance. Food Microbiol. [Epub ahead of print]; DOI: 10.1016/j.fm.2014.08.006
2.
AnguloF.J., and MølbakK.2005. Human health consequences of antimicrobial drug—resistant Salmonella and other foodborne pathogens. Clin. Infect. Dis., 41:1613–1620.
3.
BarrosM.A., NeroL.A., SilvaL.C., d'OvidioL., MonteiroF.A., TamaniniR., FagnaniR., HoferE., and BelotiV.2007. Listeria monocytogenes: occurrence in beef and identification of the main contamination points in processing plants. Meat Sci., 76:591–596.
4.
BertschD., RauJ., EugsterM.R., HaugM.C., LawsonP.A., LacroixC., and MeileL.2013. Listeria fleischmannii sp. nov., isolated from cheese. Int. J. Syst. Evol. Microbiol., 63:526–532.
5.
BoruckiM.K., and CallD.R.2003. Listeria monocytogenes serotype identification by PCR. J. Clin. Microbiol., 41:5537–5540.
6.
BuenoV.F., BanerjeeP., BanadaP.P., José de MesquitaA., Lemes-MarquesE.G., and BhuniaA.K.2010. Characterization of Listeria monocytogenes isolates of food and human origins from Brazil using molecular typing procedures and in vitro cell culture assays. Int. J. Environ. Health Res., 20:43–59.
7.
CamargoA.C., LafiscaA., CossiM.V.C., LannaF.G.P.A., DiasM.R., de ArrudaP., SérgioP., and NeroL.A.2014. Low occurrence of Listeria monocytogenes on bovine hides and carcasses in Minas Gerais State, Brazil: molecular characterization and antimicrobial resistance. J. Food Prot., 77:1148–1152.
8.
CartwrightE.J., JacksonK.A., JohnsonS.D., GravesL.M., SilkB.J., and MahonB.E.2013. Listeriosis outbreaks and associated food vehicles, United States, 1998–2008. Emerg. Infect. Dis., 19:1–9.
9.
CharpentierE., and CourvalinP.1999. Antibiotic resistance in Listeria spp. Antimicrob. Agents Chemother., 43:2103–2108.
10.
CockerillF.R.2011. Performance Standards for Antimicrobial Susceptibility Testing: Twenty-First Informational Supplement, M100S21. Clinical and Laboratory Standards Institute (CLSI).
11.
CourvalinP.2008. Predictable and unpredictable evolution of antibiotic resistance. J. Int. Med., 264:4–16.
12.
DavisJ.A., and JacksonC.R. 2009. Comparative antimicrobial susceptibility of Listeria monocytogenes, L. innocua, and L. welshimeri. Microb. Drug Resist., 15:27–32.
13.
de las HerasA., CainR.J., BieleckaM.K., and Vázquez-BolandJ.A.2011. Regulation of Listeria virulence: PrfA master and commander. Curr. Opin. Microbiol., 14:118–127.
14.
DoumithM., JacquetC., Gerner-SmidtP., GravesL.M., LoncarevicS., MathisenT., MorvanA., SalcedoC., TorpdahlM., and VazquezJ.A.2005. Multicenter validation of a multiplex PCR assay for differentiating the major Listeria monocytogenes serovars 1/2a, 1/2b, 1/2c, and 4b: toward an international standard. J. Food Prot., 68:2648–2650.
15.
FerreiraV., WiedmannM., TeixeiraP., and StasiewiczM.2014. Listeria monocytogenes persistence in food-associated environments: epidemiology, strain characteristics, and implications for public health. J. Food Prot., 77:150–170.
16.
HarakehS., SalehI., ZouhairiO., BaydounE., BarbourE., and AlwanN.2009. Antimicrobial resistance of Listeria monocytogenes isolated from dairy-based food products. Sci. Total Environ., 407:4022–4027.
17.
KhenB., LynchO., CarrollJ., McDowellD., and DuffyG.2015. Occurrence, antibiotic resistance and molecular characterization of Listeria monocytogenes in the beef chain in the republic of Ireland. Zoonoses Public Health, 62:11–17.
18.
KovacevicJ., SagertJ., WozniakA., GilmourM.W., and AllenK.J.2013. Antimicrobial resistance and co-selection phenomenon in Listeria spp. recovered from food and food production environments. Food Microbiol., 34:319–327.
19.
LiuD.Y., LawrenceM.L., AustinF.W., and AinsworthA.J.2007. A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes. J. Microbiol. Methods, 71:133–140.
20.
LundénJ., TolvanenR., and KorkealaH.2004. Human listeriosis outbreaks linked to dairy products in Europe. J. Dairy Sci., 87:E6–E12.
21.
LunguB., O'BryanC.A., MuthaiyanA., MililloS.R., JohnsonM.G., CrandallP.G., and RickeS.C.2011. Listeria monocytogenes: antibiotic resistance in food production. Foodborne Pathog. Dis., 8:569–578.
22.
MartinsE.A., and Leal GermanoP.M.2011. Listeria monocytogenes in ready-to-eat, sliced, cooked ham and salami products, marketed in the city of São Paulo, Brazil: occurrence, quantification, and serotyping. Food Control., 22:297–302.
23.
MeadP., DunneE., GravesL., WiedmannM., PatrickM., HunterS., SalehiE., MostashariF., CraigA., and MsharP.2006. Nationwide outbreak of listeriosis due to contaminated meat. Epidemiol. Infect., 134:744–751.
24.
MendonçaK.S., MichaelG.B., von LaerA.E., MenezesD.B., CardosoM.R.I., and da SilvaW.P.2012. Genetic relatedness among Listeria monocytogenes isolated in foods and food production chain in southern Rio Grande do Sul, Brazil. Food Control., 28:171–177.
25.
MorenoL.Z., PaixãoR., GobbiD.D., RaimundoD.C., FerreiraT.P., MorenoA.M., HoferE., ReisC.M., MattéG.R., and MattéM.H.2014. Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections. J. Infect. Dev. Ctries., 8:416–423.
26.
PagottoF., CorneauN., and FarberJ.2006. Listeria monocytogenes infections. In RiemannH. and CliverD. (eds.), Food-Borne Infections and Intoxications. 3rd ed., Academic Press, New York, pp. 313–340.
27.
Poyart-SalmeronC., CarlierC., Trieu-CuotP., CourvalinP., and CourtieuA.L.1990. Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet, 335:1422–1426.
28.
RagonM., WirthT., HollandtF., LavenirR., LecuitM., Le MonnierA., and BrisseS.2008. A new perspective on Listeria monocytogenes evolution. PLoS Pathog., 4:e1000146.
29.
SafdarA., and ArmstrongD.2003. Antimicrobial activities against 84 Listeria monocytogenes isolates from patients with systemic listeriosis at a comprehensive cancer center (1955–1997). J. Clin. Microbiol., 41:483–485.
30.
ShenJ., RumpL., ZhangY., ChenY., WangX., and MengJ.2013. Molecular subtyping and virulence gene analysis of Listeria monocytogenes isolates from food. Food Microbiol., 35:58–64.
31.
SwaminathanB., and Gerner-SmidtP.2007. The epidemiology of human listeriosis. Microbes Infect., 9:1236–1243.
32.
Vázquez-BolandJ.A., KuhnM., BercheP., ChakrabortyT., Domínguez-BernalG., GoebelW., González-ZornB., WehlandJ., and KreftJ.2001. Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev., 14:584–640.
33.
WangX.-M., LüX.-F., YinL., LiuH.-F., ZhangW.-J., SiW., YuS.-Y., ShaoM.-L., and LiuS.-G.2013. Occurrence and antimicrobial susceptibility of Listeria monocytogenes isolates from retail raw foods. Food Control., 32:153–158.
34.
WieczorekK., DmowskaK., and OsekJ.2012. Prevalence, characterization, and antimicrobial resistance of Listeria monocytogenes isolates from bovine hides and carcasses. Appl. Environ. Microbiol., 78:2043–2045.
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