Hirame novirhabdovirus (HIRRV) is a significant viral pathogen of Japanese flounder (Paralichthys olivaceus). In this study, seven monoclonal antibodies (mAbs) against HIRRV (isolate CA-9703) were produced and characterized. Three mAbs (1B3, 5G6, and 36D3) were able to recognize nucleoprotein (N) (42 kDa) and four mAbs (11-2D9, 15-1G9, 17F11, and 24-1C6) recognized matrix (M) protein (24 kDa) of HIRRV. Western blot, Enzyme-linked immunosorbent assay, and indirect fluorescent antibody technique (IFAT) results indicated that the developed mAbs were specific to HIRRV without any cross-reactivity against other different fish viruses and epithelioma papulosum cyprini cells. All the mAbs comprised IgG1 heavy chain and κ light chain except 5G6, which has a heavy chain of IgG2a class. These mAbs can be very useful in development of immunodiagnosis of HIRRV infection.
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References
1.
KimuraT, YoshimizuM, GorieS. A new rhabdovirus isolated in Japan from cultured hirame (Japanese flounder) Paralichthys olivaceus and ayu Plecoglossus altivelis. Dis Aquat Organ, 1986; 1(3):209–217.
2.
AmarasingheGK, AyllónMA, BàoY, et al.Taxonomy of the order Mononegavirales: Update 2019. Arch Virol, 2019; 164(7):1967–1980.
3.
NishizawaT, YoshimizuM, WintonJR, et al.Comparison of genome size and synthesis of structural proteins of hirame rhabdovirus, infectious hematopoietic necrosis virus, and viral hemorrhagic septicemia virus. Fish Pathol, 1991; 26(2):77–81.
4.
KimDH, OhHK, EouJI, et al.Complete nucleotide sequence of the hirame rhabdovirus, a pathogen of marine fish. Virus Res, 2005; 107(1):1–9.
5.
DietzgenRG, CalisherCH, KurathG, et al. (eds). Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press: London; 2011; pp. 701–713.
6.
NishizawaT, YoshimizuM, WintonJ, et al.Characterization of structural proteins of hirame rhabdovirus HRV. Dis Aquat Organ, 1991; 10:167–172.
7.
YingjieS, MinZ, HongL, et al.Analysis and characterization of the complete genomic sequence of the Chinese strain of hirame rhabdovirus. J Fish Dis, 2011; 34(2):167–171.
8.
SunY, JiangY, LiuH, et al.The isolation and characterization of a rhabdovirus from stone flounder, Kareius bicoloratus. Chin J Vet Sci, 2009; 29(3):277–282.
9.
KimWS, OhMJ. Hirame rhabdovirus (HIRRV) as the cause of a natural disease outbreak in cultured black seabream (Acanthopagrus schlegeli) in Korea. Arch Virol, 2015; 160(12):3063–3066.
10.
SeoHG, DoJ, JungS, et al.Outbreak of hirame rhabdovirus infection in cultured spotted sea bass Lateolabrax maculatus on the western coast of Korea. J Fish Dis, 2016; 39(10):1239–1246.
11.
BorzymE, MatrasM, Maj-PaluchJ, et al.First isolation of hirame rhabdovirus from freshwater fish in Europe. J Fish Dis, 2014; 37(5):423–430.
12.
OhMJ, ChoiTJ. A new rhabdovirus (HRV-like) isolated in Korea from cultured Japanese flounder Paralichthys olivaceus. J Fish Pathol, 1998; 11(2):129–136.
13.
KimWS, KimSR, KimD, et al.An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea. Aquaculture, 2009; 296(1–2):165–168.
14.
JangMS, KimJO, OhMJ, et al.Study on the tendency of viral hemorrhagic septicemia virus (VHSV) detection in Korea from 2001 to 2016 based on reported cases. J Fish Pathol, 2018; 31(1):49–55.
15.
SunY, YueZ, LiuH, et al.Development and evaluation of a sensitive and quantitative assay for hirame rhabdovirus based on quantitative RT-PCR. J Virol Methods, 2010; 169(2):391–396.
16.
BorrebaeckCA. Antibodies in diagnostics—From immunoassays to protein chips. Immunol Today, 2000; 21(8):379–382.
17.
OIE/WOAH. Manual of Diagnostic Tests for Aquatic Animals. OIE: Paris; 2006.
18.
ShyamKU, KimHJ, KoleS, et al.Antibody-based lateral flow chromatographic assays for detecting fish and shrimp pathogens: A technical review. Aquaculture, 2022; 738345.
19.
TangX, QinY, ShengX, et al.Generation, characterization and application of monoclonal antibodies against matrix protein of hirame novirhabdovirus (HIRRV) in flounder. Dis Aquat Organ, 2018; 128(3):203–213.
20.
GyeHJ, ParkMJ, KimWS, et al.Heat-denaturation of conformational structures on nervous necrosis virus for generating neutralization antibodies. Aquaculture, 2018; 484:65–70.
TowbinH, StaehelinT, GordonJ. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. PNAS, 1979; 76(9):4350–4354.
23.
KongKH, OhMJ, JangMS, et al.Development of monoclonal antibodies against viral hemorrhagic septicemia virus (VHSV, genotype IVa), the causative agent of VHS. J Fish Pathol, 2019; 32(2):59–67.
24.
LorenzenN, OlesenNJ, JørgensenP. Production and characterization of monoclonal antibodies to four Egtved virus structural proteins. Dis Aquat Organ, 1988; 4(35):e42.
25.
ArnzenJM, RistowSS, HessonCP, et al.Rapid fluorescent antibody tests for infectious hematopoeitic necrosis virus (IHNV) utilizing monoclonal antibodies to the nucleoprotein and glycoprotein. J Aquat Anim Health, 1991; 3(2):109–113.
26.
ItoT, OlesenNJ, SkallHF, et al.Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa. Dis Aquat Organ, 2010; 89(1):17–27.
27.
NishizawaT, KurathG, WintonJ. Nucleotide sequence of the 2 matrix protein genes (M1 and M2) of hirame rhabdovirus (HRV), a fish rhabdovirus. Vet Res, 1995; 26(5–6):408–412.
