Antigen sequence, corresponding to amino acid 1772–1787 of human KIF20B, NP_001271188, uniport ID: Q96Q89.
Method of Immunization
Immunized female BALA/c mice with peptide-KLH and then harvested and pooled lymphocytes from all immunized mice.
Parental Cell Line Used for Fusion
Murine SP2/0 myeloma cells.
Selection and Cloning Procedure
Fused cells plated onto selective medium and transferred clones to 96-well plate.
Primary screening performed by indirect enzyme linked immunosorbent assay on screening antigen (peptide-BSA), probing with secondary antibody for both IgG and IgM.
Secondary testing by indirect immunofluorescence (IIF), Western blotting, and addressable laser bead immunoassay (ALBIA).(1-3)
Heavy and Light Chains of Immunoglobulin
IgG1.
Specificity
Epitope mapping (overlapping 15mer peptides), IIF, Western blot, ALBIA.
Specific Antigen Identified
KIF20B peptide (QPKRAKRKLYTSEISS).
Availability
Yes
No
Tissue culture supernatant
[X]
[]
Ascitic fluid
[]
[X]
Hybridoma cells
[X]
[]
References
1.
FritzlerMJ, KerfootSM, FeasbyTE, ZochodneDW, WestendorfJM, DalmauJO, and ChanEKL: Autoantibodies from patients with idiopathic ataxia bind to M-phase phosphoprotein 1 (MPP-1). J Invest Med, 2000; 48:28–39.
2.
ZochodneDW, AuerR, and FritzlerMJ: Longstanding ataxic demyelinating polyneuropathy with a novel autoantibody. Neurology, 2003; 60:127–129.
3.
FritzlerMJ, BrownRD, and ZhangM: A monoclonal antibody to M-phase phosphoprotein 1/kinesin-like protein KIF20B. Monoclon Antib Immunodiagn Immunother, 2019; 38:162–170.
