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Abstract

Age-related macular degeneration (AMD) is a major leading cause of irreversible visual impairment in the world with limited therapeutic interventions. Histological, biochemical, genetic, and epidemiological studies strongly implicate dysregulated lipid metabolism in the retinal pigmented epithelium (RPE) in AMD pathobiology. However, effective therapies targeting lipid metabolism still need to be identified and developed for this blinding disease. To test lipid metabolism-targeting therapies, preclinical AMD mouse models are needed to establish therapeutic efficacy and the role of lipid metabolism in the development of AMD-like pathology. In this review, we provide a comprehensive overview of current AMD mouse models available to researchers that could be used to provide preclinical evidence supporting therapies targeting lipid metabolism for AMD. Based on previous studies of AMD mouse models, we discuss strategies to modulate lipid metabolism as well as examples of studies evaluating lipid-targeting therapeutics to restore lipid processing in the RPE. The use of AMD mouse models may lead to worthy lipid-targeting candidate therapies for clinical trials to prevent the blindness caused by AMD.

Introduction

Age-related macular degeneration (AMD) affects about 30% of Americans over age 70 and is the most common cause of irreversible blindness among elderly people in industrialized countries.^1–4 AMD is characterized by the progressive deterioration of the macula, an anatomical region of the retina that contains the highest density of cone photoreceptors and is responsible for visual acuity.⁵ Currently, there are limited interventions that slow or prevent the progression of AMD to its blinding late stages, thus there is an urgency to identify therapies that prevent or delay its progression. AMD is a complex, progressive, retinal degenerative disease influenced by both environmental and genetic factors and is dependent upon advanced age.⁶ It is imperative to devise therapeutic strategies for AMD and curb its burden on societies since the prevalence of AMD is expected to substantially increase in the next decades.¹

An ideal strategy to treat AMD would involve targeting the early/intermediate “dry” stages before irreparable visual loss occurs in patients. The early stages of AMD are fairly benign and consist of impaired dark adaptation that corresponds to rod photoreceptor dysfunction,⁷ focal accumulation of intermediate-sized (63 to 125 μm diameter) lipid, lipoprotein, and protein-containing deposits known as drusen within Bruch's membrane (BrM),⁸ and choriocapillaris dropout.⁹ Intermediate AMD comprises retinal pigmented epithelium (RPE) pigmentary changes¹⁰ and larger drusen (>125 μm diameter).⁸ As AMD progresses to its late stages, it is classified as either exudative or nonexudative AMD. The presence of choroidal neovascularization (CNV) characterizes the exudative or “wet” form of late AMD where the formation of immature blood vessels from the choroid advances into the RPE and subretinal space causing fibrosis and scarring and irreparable vision loss.¹¹ The nonexudative or “dry” form of late AMD is distinguished by geographic atrophy where there is central loss of RPE and photoreceptors without the presence of vascular leakage.⁶ Most of the AMD-afflicted population has early/intermediate or late “dry” AMD, while 10% of patients present with “wet” AMD.⁶

Current therapies for AMD target pathologies associated with the wet form of AMD, whereas no therapies exist for early/intermediate dry AMD or geographic atrophy. The current gold standard for the treatment of wet AMD is intravitreal injection of antivascular endothelial growth factor (VEGF) antibodies, such as bevacizumab (Avastin) and ranibizumab (Lucentis) or the soluble decoy receptor targeting VEGF-A, aflibercept (Eylea).^12,13 However, not all wet AMD patients respond well to anti-VEGF therapies.¹⁴ It is also possible for patients with exudative AMD to progress to late stages of nonexudative AMD even after anti-VEGF treatment.¹⁵ Currently, the only intervention available for the treatment of dry AMD is Age-Related Eye Disease Supplement (AREDS), an oral supplement containing vitamin C, vitamin E, lutein/zeaxanthin, and zinc. AREDS enhances protection against oxidative stresses in the eye and was shown in the AREDS trials to reduce the risk of advanced AMD by about 25% over a 5-year period in participants with intermediate AMD, although there was no effect in participants with early or no AMD.^16,17

Development of novel therapies targeting dry AMD should be facilitated by identifying and examining the pathobiological processes implicated in AMD. Inflammation, complement dysregulation, oxidative stress, extracellular matrix (ECM) remodeling, dysregulated lipid metabolism, and angiogenesis have been implicated as key pathobiological mechanisms underlying AMD development and progression.¹¹ Polymorphisms in genes that regulate complement activation and lipid metabolism are among the biggest genetic risk factors for AMD.¹⁸ Strong evidence based on biochemical, genetic, and cell biological studies implicates the alternative pathway of complement in the development of AMD.^18,19 Growing evidence from studies using preclinical AMD mouse models further supports an important role of dysregulated lipid metabolism in AMD-like pathology development in vivo and provides a potential link between complement factors and lipoprotein accumulation and clearance.^20–23 In this review, we present an overview of mouse models of AMD, present new strategies for modulating lipid metabolism based on findings from AMD mouse model studies, and summarize studies that evaluated treatments focused on lipid metabolism in AMD mouse models. For more comprehensive reviews of lipid metabolism in AMD, we refer readers to these excellent reviews.^24–27 In addition, we direct readers to this excellent review that highlights higher order animal models of AMD as these models will not be discussed in this review.²⁸

Associations of Dysregulated Lipid Metabolism with AMD

Risk factors for developing AMD include advanced aging, genetic variants, and environmental stressors. Advanced aging is the strongest associated risk factor for AMD (Fig. 1).^29–31 An individual's risk for developing AMD at age 55 is 0.7%, but this exponentially increases to 22.5% by the age of 80.³² Aging induces a number of changes in the retina, including loss of mitochondria,³³ RPE pigmentary changes,¹⁰ and accumulation of lipid within BrM, a pentalaminar ECM that separates the RPE from its adjacent choroidal blood supply and acts as a basement membrane for the RPE and choroid (Fig. 1).³⁴ The age-dependent accumulation of lipid in BrM is thought to contribute to the development of drusen.³⁴ While the presence of a few small “hard” drusen or basal laminar deposits (BLamD) is a normal, nonvision-impairing part of aging, the deposition of large diffuse drusen, or basal linear deposits, in the macula is vision impairing and indicative of intermediate AMD.³⁴

FIG. 1.

Association of dysregulated lipid metabolism with AMD development and progression. Left: Normal, healthy retina. Middle: Retina with signs of benign aging such as loss of mitochondria, RPE pigmentary changes, and BLamD formation between the RPE and BrM. Right: Through a complex interplay between genetic variants and environmental factors, AMD can develop and progress in the aging retina. Classic pathological hallmarks of AMD include recruitment of subretinal immune cells, photoreceptor degeneration, RPE atrophy and loss, drusen (which start out as basal linear deposits), and form within the ICL of BrM, choroidal atrophy, and CNV. Genetic and epidemiological studies of AMD patients revealed dysregulated lipid transport and metabolism as a key pathobiological mechanism behind AMD. Coding variants in APOE, LIPC, CETP, LPL, and ABCA1 are associated with AMD risk, but it is still unknown why particular variants are linked with disease risk. In addition, diets enriched in omega-6 fatty acids while those deficient in omega-3 fatty acids also increase an individual's risk for AMD. It is widely accepted that omega-6 fatty acids promote, and omega-3 fatty acids dampen retinal inflammation. ABCA1, ATP-binding cassette subfamily A member 1; AMD, age-related macular degeneration; APOE, Apolipoprotein E; BI, basal infoldings; BLamD, basal laminar deposit; BrM, Bruch's membrane; CETP, cholesteryl ester transfer protein; Choroid BM, choroid basement membrane; CNV, choroidal neovascularization; EL, elastic layer; ICL, inner collagenous layer; LD, lipid droplet; LIPC, hepatic lipase; LPL, lipoprotein lipase; M, mitochondria; N, nucleus; OCL, outer collagenous layer; PG, pigment granule; Ph, phagosome; RPE BM, RPE basement membrane; RPE, retinal pigmented epithelium.

Lipid accumulation and cholesterol have long been implicated in AMD disease development.^35,36 Historically, a role for lipids in AMD has been established through pathohistological examinations of drusen in human donor eyes.³⁶ Immunofluorescence, mass spectrometry, and transcript studies of human donor eyes identified apolipoprotein E (ApoE) as a major drusen constituent.^37–41 ApoE is a protein component of most lipoproteins, lipid-, and protein-containing complexes responsible for packaging cholesterol and fats from the circulation for transport to tissues and removing cholesterol and lipids from tissues through reverse cholesterol transport. The function of ApoE in lipoproteins is to facilitate their cellular uptake as well as bind to extracellular matrices for efficient delivery and removal of lipids from cells.⁴² In addition to ApoE, a number of lipid species such as esterified cholesterol,^40,43,44 unesterified cholesterol,^40,44 phosphatidylcholine,⁴⁰ triglycerides,⁴⁰ sphingomyelin,⁴⁰ and fatty acids,⁴⁰ as well as other apolipoproteins^40,41 have been identified as drusen components. Many of these drusen components are oxidized,⁴⁵ and these oxidized lipids and proteins are believed to contribute to a proinflammatory environment that accelerates AMD development.⁴⁶

The origin of ApoE-containing lipoproteins in drusen is from systemic sources through the circulation and from local synthesis by the RPE, although the relative contribution of each is not known. Circulating lipoproteins must enter and pass through BrM, which is thought to allow passive diffusion of high-density lipoproteins (HDLs) and low-density lipoproteins (LDLs) based on hydraulic conductivity and LDL diffusion studies using bovine BrM explants.⁴⁷ Intriguingly, increased plasma HDLs^48–52 and LDLs⁵³ have been associated with increased risk for AMD and may directly impact drusen accumulation and growth. Evidence that locally derived ApoE-containing lipoproteins contribute to drusen biogenesis comes from studies showing that human RPE cells are capable of synthesizing and secreting ApoE.^54,55 In addition, a cell culture model of drusen biogenesis developed by Johnson et al. using primary human RPE cells exposed to human serum that mimics several aspects of early AMD showed accumulation of drusen-like deposits that contain ApoE and other drusen-associated proteins.⁵⁶

Genetic variants are strong contributors to AMD accounting for 28%–43% of AMD disease risk depending on the estimated prevalence of AMD in the population.^18,57 Variants in genes involved in lipid metabolism include APOE. ApoE exists as 3 isoforms that differ at 2 amino acid positions: ApoE2 (Cys112, Cys158), ApoE3 (Cys112, Arg158), and ApoE4 (Arg112, Arg158).⁵⁸ The APOE2 isoform is associated with increased risk for AMD while the APOE4 isoform is mildly protective against AMD.^59–62 Variants in other genes involved in lipid metabolism that are associated with AMD risk include hepatic lipase (LIPC), cholesteryl ester transfer protein (CETP), and lipoprotein lipase (LPL), which are involved in HDL cholesterol metabolism, and ATP-binding cassette subfamily A member 1 (ABCA1) (Fig. 1).^63–65 The importance of HDL cholesterol metabolism in the RPE is highlighted by a recent phenotypic study of mice with RPE-specific ablation of ABCA1 and ATP-binding cassette transporter G1 (ABCG1).⁶⁶ The RPE-specific ablation of ABCA1 but not ABCG1 was sufficient to cause lipid accumulation in the RPE, degeneration of the RPE and photoreceptors, and visual loss in mice.⁶⁶ Interestingly, the AMD-associated genetic variant of ABCA1 decreases its expression in RPE cells.⁶⁶ ABCA1 and ABCG1 are critical exporters of cholesterol from cells,⁶⁷ suggesting the molecular explanation of the risk associated with ABCA1 variants and AMD may be due to decreased export of cholesterol from RPE cells.

Environmental and lifestyle stressors modulate the effects of aging and genetic variants on AMD development. Cigarette smoking strongly influences the risk for AMD, whereas obesity, hypertension, sunlight exposure, and alcohol consumption are mildly associated with disease risk.^68–73 A high-fat (HF) diet is an established risk factor for AMD^74–76 and often used in AMD mouse model studies.^{20,21,77–84} Consuming diets with a high concentration of fish oils containing omega-3 fatty acids are related with a decreased incidence of AMD^85–88 compared with increased incidence of AMD in individuals eating diets high in omega-6, monounsaturated, polyunsaturated, and trans unsaturated fatty acids (Fig. 1).^86,87 It is largely unknown how these environmental stressors interact with advanced aging and genetics to influence AMD pathogenesis.

Models of AMD

AMD can be modeled using a variety of organisms that range from zebrafish to mice to nonhuman primates. Nonhuman primates are ideal models due to the presence of a macula, drusen in aged animals,⁸⁹ and shared common AMD susceptibility genes such as age-related maculopathy susceptibility 2 (ARMS2)/HtrA serine peptidase 1 (HTRA1),⁹⁰ but these animals are very costly to maintain and have a slow disease progression. Nonmammalian models like zebrafish are advantageous in that they produce large quantities of offspring and allow for easy assessment of eye phenotypes due to their transparent bodies in juveniles, but they differ from mammals in their retinal vasculature organization and develop photoreceptor degenerations beginning in the larval stage and not in the adult fish.^91,92

Mice are the most routinely used model organisms for studying AMD due to their short life span, genetic and pharmacologic manipulability, inexpensive housing, and retinal architecture that is similar to humans. The mouse retina is particularly susceptible to the development of age-dependent retinal pathologies, such as decreased visual function,^93,94 BLamD formation,⁹⁵ RPE multinucleation,⁹⁶ cataract formation,⁹⁷ ectopic synapse development,⁹⁸ and neuroinflammation,^98,99 validating their usage in interrogating mechanisms associated with age-dependent retinal diseases like AMD.

The most commonly used mouse models in AMD research largely represent early and intermediate dry AMD, although there are a few models that aim to model wet or late dry AMD (Fig. 2 and Table 1). One important consideration for an AMD mouse model is the incorporation of advanced aging, as aging is the strongest risk factor for human AMD. Many early and intermediate dry AMD mouse models incorporate advanced aging, thus effectively incorporating the effect of chronic processes that drive the development of early/intermediate AMD pathology.^{20,21,77,80,81,83,100–124} In contrast, models of late AMD stages are often based on acute insults to young mice that consequently develop retinal pathology in a short time frame (e.g., 1 to 2 weeks).^125–127 Therefore, these models may not faithfully reflect the complex age-dependent pathological cues and/or mechanisms that cause AMD and may confound the interpretation of drug efficacy studies.

FIG. 2.

Mouse models of AMD. AMD mouse models can be divided up into 5 categories, including mouse models of “early” and “intermediate” AMD, mouse models of neovascular AMD, mouse models of geographic atrophy, acute mouse models of AMD, and mouse models of inherited macular degeneration. Mouse models highlighted in red are the ones used to test the role of lipids in AMD-like pathology development. For more information on these models, we refer readers to Table 1.

Table 1.

Summary of Current Age-Related Macular Degeneration Mouse Models

	Mouse model	Abbreviation	Comments	References
Mouse models of “early” and “intermediate” AMD	Acid sphingomyelinase (ASMase) knockout (KO) mouse	ASMase⁻ ^/−	Deficiency of ASMase leads to age-dependent visual loss, photoreceptor degeneration, and increased RPE autofluorescence that correlates with decreased retinal sphingomyelin levels and increased eyecup autophagy.	¹³¹
	Human apolipoprotein B100 (APOB100) mouse fed a high-fat (HF) diet	APOB100∼HF	C57BL/6J mice that contain the full-length human APOB gene were fed a HF diet and aged to 12 months but no apparent phenotypic differences were observed between APOB100 mice fed a normal mouse chow and HF diet.	⁷⁷
	Apob100 mouse fed a low-fat (LF) diet	Apob100∼LF	Mice with Apob mutation that prevents the formation of the alternatively spliced Apob48 variant was used to evaluate LXR agonists.	^100,101
	Apolipoprotein E (Apoe) KO mouse fed a high cholesterol (HC) diet	Apoe^−/−∼HC	4–5-week-old Apoe^−/− mice were fed a HC diet for 25 weeks and had worsened retinal pathologies relative to controls.	^78,79
	Human apolipoprotein E isoform 4 (APOE4) mouse fed a high-fat, cholesterol-enriched (HFC) diet	APOE4∼HFC	Aged APOE4∼HFC develop more severe AMD-like pathology than aged APOE2 and APOE3 mice fed a HFC diet.	^80,81
	Aryl hydrocarbon receptor (Ahr) KO mouse	Ahr⁻ ^/−	Chronic mouse model based on AHR activity and protein concentration decreases with age in human RPE cells.¹⁰²	¹⁰²
	C57BL/6J mouse exposed to cigarette smoke (CS)	C57BL/6J∼CS	2-month-old C57BL/6J mice were exposed to cigarette smoke for 5 h a day and 5 days a week for 6 months that resulted in ultrastructural changes to BrM and RPE apoptosis.	¹³²
	C57BL/6J mouse fed a HF diet	C57BL/6J∼HF	6-week-old C57BL/6 mice fed a HF diet for 30 weeks developed decreased ERGs, increased fundus abnormalities and thickened BrM.	⁸²
	C57BL/6J mouse fed a high glycemic (HG) diet	C57BL/6J∼HG	16-month-old C57BL/6J mice were fed a HG diet until 23.5 months of age and developed more AMD-like pathologies that correlated with changes in the microbiome.¹⁰³	^103,104
	Complement membrane cofactor protein (Cd46) KO mouse	Cd46^−/−	Loss of CD46, a membrane regulator of complement activation, leads to age-dependent mice increases in RPE autofluorescence and BrM thickness as well as decreases in choroidal thickness in mice.	¹²⁴
	C57BL/6J mouse immunized with carboxyethylpyrrole (CEP)-adducted BSA	CEP immunized C57BL/6J	Chronic mouse model with increased inflammasome activation¹³³ and increased proinflammatory macrophage infiltration.¹³⁴	¹⁰⁵
	Collagen-induced arthritis (CIA) in C57BL/6J mouse	CIA Induced C57BL/6J	Mouse model of systemic inflammation that had decreased laser-induced CNV lesion size but worsened RPE pathologies after sodium iodate treatment.	¹³⁵
	Ceruloplasmin (cp) and hephaestin (Heph) double KO mouse	Cp^−/− Heph⁻ ^/−	Chronic mouse model in which pathologies result from increased oxidative stress from retinal iron overload as supported by the protection against pathologies with the iron chelator deferiprone.¹³⁶	^106,107
	Complement factor h (Cfh) KO mouse	Cfh⁻ ^/−	Absence of CFH leads to excess complement activation resulting in no reservoir of plasma complement proteins and subtle retinal damage in aged Cfh^−/− mice.	^108,109,137
	Heterozygous Cfh KO mouse fed a HFC diet	Cfh^+/−∼HFC	Aged Cfh^+/−∼HFC provides the first multifactorial AMD mouse model suited to test the role of complement components on AMD-like pathology development such as C5a.¹³⁸	²¹
	Human complement factor H (CFH) Y402 and H402 mouse	CFH,Cfh^−/−	Transgenic mice expressing the full-length human CFH gene encoding either the Tyrosine (Y) or Histidine (H) at amino acid 402 were crossed to Cfh^−/− mice to generate mice that produce only human CFH Y402 or H402 protein, respectively, which functions with mouse complement proteins.	¹⁰⁹
	Human complement factor H (CFH) Y402 and H402 mouse aged and fed a HFC diet	CFH∼HFC	Phenotypic differences in ocular phenotypes, circulating lipoproteins and ocular lipoproteins were noted between mice expressing equal concentrations of either the CFH Y402 or H402 variant. Only CFH-H/H develop AMD-like phenotype	²⁰
	Chimeric, transgenic Cfh mouse	Chimeric Cfh	Mouse Cfh was genetically modified to include short consensus repeat domains 6 through 8 of human CFH and included either the Y402 or H402 amino acid but no difference in phenotype was observed between mice expressing these variants.	¹¹⁰
	Mutant Cfh and Complement factor P (fP) KO mouse	fH^m/m, fP^−/−	Mice with a premature stop codon in the end of exon 19 of the mouse Cfh gene that results in complement activation¹³⁹ crossed to fP KO mice generated increased sub-RPE basal deposits at an early age.	¹⁴⁰
	C-X-C chemokine receptor type 5 (Cxcr5) KO mouse	Cxcr5^−/−	Cxcr5^−/− mice have increased sub-RPE deposits containing amyloid beta and C3a¹¹¹ that may result from disrupted PI3K/AKT signaling and FOXO1 upregulation.¹¹²	¹¹³
	Cxcr5 and nuclear factor-like 2 (Nrf2) double KO mouse	Cxcr5^−/−,Nrf2^−/−	Combined deficiency of Cxcr5 and Nrf2 worsens AMD-like pathologies observed in both Cxcr5 and Nrf2 KO mice.	¹⁴¹
	Lysosome-associated membrane protein-2 (Lamp2) KO mouse	Lamp2^−/−	Absence of LAMP2 in mice leads to the acceleration of sub-RPE basal laminar deposits that contain extracellular matrix proteins, lipoproteins, and cholesterol.	¹¹⁴
	Microtubule-associated protein 1 light chain 3 B (LC3B) KO mouse	LC3B^−/−	LC3B KO mice have increased phagosomes, decreased fatty acid oxidation, RPE lipid accumulation, and subretinal immune cell infiltration.	¹⁴²
	Nrf2 KO mouse fed a HF diet	Nrf2⁻^/−∼HF	12-month-old Nrf^−/− mice fed a HF diet for 16 weeks developed a more robust AMD-like phenotype that correlated with interleukin 17-producing γΔ T cells.	⁸³
	Heterozygous peroxisome proliferator-activated receptor-γ coactivator 1α (Pgc1α) KO Mouse Fed a HF Diet	Pgc1α^+/−∼HF	Combined Pgc1α heterozygosity and consumption of a HF diet leads to loss of choroidal fenestrations and increased expression of drusen-associated genes.	⁸⁴
	Pgc1α and Nrf2 double KO mouse	Pgc1α ^−/−,Nrf2^−/−	Combined deficiency of Pgc1α and Nrf2 causes RPE abnormalities and subRPE basal deposits that correlate with damaged mitochondria and increased ER stress.	¹⁴³
	Peroxisome proliferator-activated receptor-β/δ (Pparβ/δ) KO mouse	Pparβ/δ^−/−	Pparβ/δ deficiency leads to increased lipid accumulation and thickened BrM but attenuates laser-induced CNV lesion size.	¹²³
	RPE-specific ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) mouse	RPE ^{ΔAbca1;Abcg1}	Absence of ABCA1 and ABCG1 in the RPE leads to increased lipids within the RPE, causing RPE dysmorphogenesis, neuroinflammation, and photoreceptor degeneration.	⁶⁶
	RPE-specific KO of RB1-inducible coiled-coil 1 (Rb1cc1) mouse	Rb1cc1-CKO	Loss of RB1CC1 in mouse RPE leads to autophagy defects and pathologies, including RPE degeneration, subretinal immune cell infiltration, subRPE deposition of inflammatory and oxidatively damaged proteins, subretinal drusenoid deposits, and CNV that precedes neural retinal abnormalities.	¹⁴⁴
	Rod-specific tuberous sclerosis complex 1 (Tsc1) KO mouse	^rodTsc1^−/−	Mice with rod-specific Tsc1 ablation that leads to constitutive activation of mTORC1 and pathologies resembling those observed in early, late dry, and wet AMD.	¹²²
	Superoxide dismutase 1 (Sod1) KO mouse	Sod1⁻ ^/−	Chronic mouse model with a susceptibility to oxidative stress damage as shown by increased retinal damage after an intravitreal injection of paraquat.¹⁴⁵	^115,116
	Superoxide dismutase 2 (Sod2) knockdown mouse	Sod2^−/−	Chronic mouse model where Sod2 has been knocked down using a viral-delivered ribozyme and Cre-LoxP recombination that has been used to test the efficacy of RPE65-programmed bone marrow-derived cells in vivo.¹⁴⁶	^117,118
Mouse models of neovascular AMD	Htra serine peptidase 1 (HTRA1) overexpressing mouse	HTRA1	HTRA1 overexpression was achieved by RPE65 promoter-driven mouse Htra1,¹²⁹ CMV-BEST1 hybrid promoter-driven human HTRA1¹²⁸ and CAG-driven mouse Htra1¹¹⁹ expression in wild-type mice and resulted in pathologies after 1 year of age	^119,128,129
	Htra1 overexpressing mouse exposed to cigarette smoke (CS)	HTRA1∼CS	12-month-old HTRA1 overexpressing mice were exposed to cigarette smoke for 30 min per day, 5 days per week for 12 weeks, and resulted in increased CNV and sub-RPE basal deposits.	¹¹⁹
	Laser-induced CNV		Commonly used acute model that has been interrogated in multiple transgenic mice (i.e., Cfh^−/−,¹³⁷ Ahr^−/−¹⁴⁷, and Cx3cr1^−/−¹⁴⁸ and used for testing novel therapies for wet AMD.	^127,149
	Polyethylene glycol (PEG)-induced CNV		Subretinal injection of PEG, a complement activator, leads to CNV in mice 5 days postinjection and is dependent on complement activation.	¹⁵⁰
	Very-low-density lipoprotein receptor (Vldlr) KO mouse	Vldlr ^−/−	Mouse used to model retinal angiomatous proliferation.	¹⁵¹
Mouse models of geographic atrophy	Dicer knockdown mouse	Dicer ^−/−	Acute mouse model where Dicer has been knocked down by Cre-LoxP recombination and results in increased Alu mRNA and frank RPE cell death mediated by inflammasome activation.¹⁵²	^120,153
	Inducible Cre recombinase driven by the monocarboxylate transporter 3 promoter and Diphtheria toxin A (DTA) with LoxP-flanked stop codon double transgenic mouse	RPE(CreER)/DTA	RPE(CreER)/DTA mice have 60%–80% RPE cell death that results in ERG and retinal pathology and serves as a valuable model for stem cell-derived RPE transplantation studies.	¹⁵⁴
	Sodium iodate treatment		Acute insult causing RPE damage resulting in RPE atrophy and death, retinal degeneration, and immune cell recruitment by 3 days postinjection.	^125,126,155
	Laser-induced RPE atrophy		Acute mouse model with focal atrophic photoreceptors, abnormal RPE and BrM, visual loss, and neuroinflammation without signs of neovascularization.	¹⁵⁶
	Transmembrane protein 135 (Tmem135) transgenic (TG) mouse	Tmem135 TG	Overexpression of Tmem135 leads to fragmented mitochondria in RPE cells as well as progressive RPE degeneration and dysmorphogenesis without affecting visual function until 1 year of age in mice.	¹²¹
Acute models of AMD	Blue light exposure		Acute insult that results in damage to the neural retina that has been used to differentiate microglia and bone marrow-derived macrophages in the retina.¹⁵⁷	^158–160
	Intravitreal injection of paraquat		Acute insult that results in increased oxidative stress damage and subsequent damage to the neural retina that is used for antioxidant therapy studies.	¹⁶¹
	Oral hydroquinone		16-month-old C57BL/6J female mice were given oral hydroquinone in their drinking water that resulted in the development of sub-RPE basal deposits.	¹⁶²
	White light exposure		BALB/c albino mice treated with white light for 24 h leads to photoreceptor apoptosis and visual loss¹⁶³ as well as immune cell infiltration that is worsened after CEP immunization.¹³⁴	¹⁶³
Mouse models of inherited macular degeneration	ATP-binding cassette, sub-family A (ABC1), member 4 (Abca4) KO mouse	Abca4⁻ ^/−	Mouse model of recessive Stargardt's disease that is often used in studies on complement activation,¹⁶⁴ lipofuscin, and novel drug development for AMD.¹⁶⁵	^166,167
	C1q and tumor necrosis factor related protein 5 (C1QTNF5) serine to arginine at amino acid 163 (S163R) knockin mouse	C1QTNF5^S163R	Mouse model of late-onset retinal degeneration generated by an introduction of the S163R mutation into the mouse C1QTNF5 gene¹⁶⁸ as well as viral delivery of human C1QTNF5^S163R to the RPE of C57BL/6J mice.¹⁶⁹	^168,169
	EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1) arginine to tryptophan at amino acid 345 (R345W) knockin mouse	Efemp1^R345W	Mouse model of Malattia Leventinese/Doyne's Honeycomb Dystrophy revealed a vital role of complement in sub-RPE deposit formation¹⁷⁰ and replicated using cell culture.¹⁷¹	^172,173
	Elongation of very-long-chain fatty acids protein 4 (Elovl4) five base pair deletion knockin mouse	Elovl4^mut/−	Mouse model of dominant Stargardt's disease characterized by defects in very-long-chain polyunsaturated fatty acids in the retina.¹⁷⁴	^175,176
	Metalloproteinase inhibitor 3 (Timp3) serine to cysteine at amino acid 156 (S156C) knockin mouse	Timp3^S156C	A mouse model of Sorsby's dystrophy used in angiogenesis studies.¹⁷⁷	¹⁷⁸

AMD, age-related macular degeneration; BrM, Bruch's membrane; CNV, choroidal neovascularization; ERG, electroretinography; LXR, liver X receptor; mTOR, mammalian target of rapamycin; RPE, retinal pigmented epithelium.

One exception is the wet AMD model using aged HTRA1-overexpressing mice where pathologies were observed at 1 year of age.^119,128,129 An important issue to consider is that AMD-associated variants at chromosome 10q26 loci, where HTRA1 is located, have been shown to decrease HTRA1 expression, suggesting HTRA1-overexpressing mice may not represent the role of HTRA1 in AMD pathobiology.¹³⁰ This is further supported by a recent article from the Hageman group showing that HtrA1 is specifically reduced as much as 50% in the RPE of humans with the ARMS2 risk allele.¹³⁰ This appears to be due to disruption of a cis-acting regulatory element within the ARMS2 locus further supporting that augmentation, not inhibition, of HTRA1 is a rational therapeutic approach for AMD patients with the 10q26 risk allele.¹³⁰

A popular strategy for generating AMD mouse models has been to subject them to acute insults aimed at reproducing AMD-relevant environmental stressors and assessing retinal damage after a few days and/or weeks. Because oxidative stress has been implicated in AMD, many of these acute insults increase the oxidative stress burden in the eye. These include systemic sodium iodate treatment,^125,126 intravitreal paraquat,¹⁶¹ oral hydroquinone,¹⁶² and blue light exposure^158–160 (Fig. 2 and Table 1).

The most commonly used acute AMD mouse model is laser-induced CNV, which is meant to model wet or neovascular AMD¹²⁷ (Table 1). Importantly, it has been a reliable animal surrogate in the development of therapeutics to treat wet AMD, including predicting the clinical efficacy of anti-VEGF therapy for wet AMD.¹⁴⁹ Laser photocoagulation is used to disrupt BrM in laser-induced CNV, stimulating growth of new choroidal blood vessels toward the retina.¹²⁷ The laser-induced CNV mouse model produces quick results within a few weeks, but it is more of a wound-healing model.¹⁷⁹ Unlike in human CNV, the laser-induced CNV in rodents spontaneously regresses after a few weeks and there is considerable variation in outcome between mouse strains, genotypes, and age.¹⁸⁰ Still, the laser-induced CNV mouse model has been widely used in studies evaluating therapies targeting components of lipid metabolism for exudative AMD, such as omega-3 long-chain polyunsaturated fatty acids (LCPUFAs),^181–184 apolipoprotein A-I (ApoA1) and ApoAI-binding protein (AIBP),¹⁸⁵ apolipoprotein M (ApoM),¹⁸⁶ HDL eye drops,¹⁸⁷ AREDS2 supplementation,¹⁸⁸ atorvastatin,¹⁸⁹ pitavastatin,¹⁹⁰ and cytochrome P450 oxidase 2C (CYP2C) inhibition.¹⁹¹

The use of mouse models of inherited macular degenerations may be advantageous to test preclinical AMD therapeutics due to their quicker onset, robust penetrance, and severe pathology development. A subset of mouse models were developed based on mutations in genes that cause inherited macular degenerations in humans, including C1q and tumor necrosis factor-related protein 5 (C1QTNF5) in late-onset retinal macular degeneration,^168,169 EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) in Malattia Leventinese/Doyne's honeycomb retinal dystrophy,^172,173 metalloproteinase inhibitor 3 (TIMP3) in Sorsby's fundus dystrophy,¹⁷⁸ and ATP-binding cassette subfamily A member 4 (ABCA4) in autosomal recessive Stargardt disease (STGD1) or elongation of very-long-chain fatty acid protein 4 (ELOVL4) in autosomal dominant Stargardt macular dystrophy (STGD3)^{166,167,175,176} (Fig. 2 and Table 1). Inherited macular degenerations differ from AMD since they are caused by a genetic mutation and generally present earlier in life.^192–196 Still, regardless of the different etiologies between these diseases, the mouse models of these macular degenerations recapitulate many of the cardinal features of their respective inherited macular degeneration and some features of AMD.^{166,168,172,173,175,176,178,197,198}

Limitations of Mouse Models in AMD and Lipid Metabolic Research

Although there are many advantages to using preclinical AMD mouse models, it is critical that AMD researchers be well informed of the potential confounders that might influence the interpretation of AMD mouse model studies. For example, there are genetic mutations within some mouse strains that can cause retinal degenerations such as the rd8 mutation in Crumbs homolog 1 (Crb1)¹⁹⁹ and rd1 mutation in phosphodiesterase 6B (Pde6b).^200,201 Before beginning any study, researchers should screen their mice to confirm the absence of these mutations in their colonies. Another important consideration of AMD mouse models is the genetic background of the mouse. It is becoming apparent that the phenotypes of murine disease models are heavily dictated by their mouse genetic background as observed with mouse models of Alzheimer's disease.²⁰² Lastly, environmental variation can also exert unwanted effects on AMD mouse model studies. Differences in normal mouse chow, microbiota, and stress levels can affect phenotypic outcome measures.²⁰³ Consideration, careful planning, and appropriate controls are crucial determinants for the success of any study using AMD mouse models. Here are some of the disadvantages of using preclinical AMD mouse models based on differences in the anatomy of their visual system and lipid metabolism.

Differences between mouse and human retinas

Notable distinctions exist between the mouse and human retina. For the most part, mouse and human eyes undergo similar early developmental programs, but these programs diverge to allow for the complex structure of the human retina.²⁰⁴ Importantly, mice do not possess a cone-dominant macular region but rather a rod-rich retina that is similar to the rod-rich parafovea in humans.²⁰⁵ This similarity is an advantage of using the mouse retina for modeling early to intermediate AMD since this rod-rich region of the human macula is where macular degeneration is first detected.²⁰⁶ The cone-dominant macular region in the human retina allows for high visual acuity, but mice lacking this type of retinal structure have extremely low visual acuity that is equivalent to 20/2,000.²⁰⁷ Rods and cones have different energy demands²⁰⁸ and transcriptional profiles,²⁰⁹ thus, there could be difficulty in translating findings of ophthalmic drug studies in mice to humans.

A potent physiological difference between a mouse and human retina is the interaction between photoreceptors and RPE cells. RPE cells are tasked with delivering nutrients to photoreceptors and ingesting photoreceptor outer segment wastes.²¹⁰ The central part of the mouse retina contains a higher density of photoreceptors than the central part of the human retina, equating to a higher proportion of photoreceptors to RPE cells in the central murine retina.²⁰⁵ In addition, there are more smaller and denser RPE cells in the center of the mouse retina than the central human retina.²⁰⁵ These differences suggest that murine RPE may have increased phagocytosis of photoreceptor outer segments and may explain transcriptional differences between mouse and human RPE, including increased expression of oxidative stress and outer retinal barrier genes.²¹¹ It has been shown that mice have higher basal metabolic rates than humans²¹² and could suggest higher metabolic rates in mouse RPE cells than human RPE cells. Furthermore, since RPE cells have coordinated metabolic relationships with both rod and cone photoreceptors,²¹³ it is plausible that there are variations in the metabolic ecosystem within the murine retina versus human retina and may confound therapeutic studies, especially those focusing on metabolism.

Differences between mouse and human lipid metabolism

Mice and humans have similar expression of genes involved in lipid metabolism within the retina,^214,215 but it is unknown if there are differences between lipid metabolic functions between mouse and human RPE. One important expression difference between these species is the absence of CETP in the mouse.²¹⁶ CETP facilitates the transfer of cholesterol between HDL to very-low-density lipoprotein (VLDL) and LDL.²¹⁷ Because of the CETP deficiency in mice, most plasma cholesterol is confined to HDL particles.²¹⁸ This is in stark contrast to humans where most plasma cholesterol is found in LDL particles.²¹⁸ Recapitulating the plasma lipid profiles of humans in mice can be achieved by diet intervention but often these diets do not represent typical diets consumed by humans. Strikingly, regardless of the absence of CETP and diet intervention, both mice and humans have comparable lipoprotein proteomes that may indicate similar functions of lipoproteins between mice and humans.²¹⁸ Lastly, differences in transcription factors involved in lipid metabolism between mice and humans may influence lipid-targeting drug studies in AMD mouse models.²¹⁹ Thus, caution is warranted when extrapolating conclusions from lipid metabolism, notably with regard to cholesterol metabolism, studies in mice to humans.

Translatability of mouse studies to the clinic

The anatomical differences between the mouse and human retina have called into question the usefulness of mice in evaluating therapies for AMD since these studies may not translate well to the clinic. However, it is important to remember that AMD mouse models are only 1 step in the bench-to-bedside pipeline for translating basic research to clinical treatments (Fig. 3). We strongly advocate for the validation of preclinical AMD mouse model studies with other models of AMD. In parallel with the mouse models presented in this review, advances have been made in cell-culture systems such as fetal human RPE,²²⁰ induced-pluripotent stem cell-derived RPE,²²¹ primary porcine RPE,²²² and retinal organoids²²³ that allow for confirmation of mouse study findings in human cells (Fig. 3). Ultimately, any therapeutic showing promising effects should be tested in higher order animal models of AMD, including rats, rabbits, pigs, or nonhuman primates (Fig. 3). No model of AMD has represented the full complex spectrum of AMD but combining multiple models together is essential for the translating results from mouse studies to humans.

FIG. 3.

Bench-to-bedside pipeline of AMD therapeutics. Preclinical AMD mouse models can be used to evaluate therapeutics for AMD but these therapeutics need to be tested in cell cultures modeling AMD and/or higher order animal models of AMD. Then, promising therapies can advance to clinical trials to determine if they are viable treatments for AMD. Multiple models should be utilized to translate preclinical AMD mouse model studies to clinically relevant applications.

Devising and Testing Lipid Metabolism-Targeting Therapies in Preclinical AMD Mouse Models

Numerous AMD mouse models are available to researchers, but it can be a daunting task to best leverage these models to design and determine drug efficacy of therapies targeting lipid metabolism. We propose 3 major therapeutic goals based on previously published AMD mouse model studies: (1) clearance of pathogenic lipid or protein components in sub-RPE deposits, (2) restoration of lipid processing in the RPE and BrM, and (3) preservation of lipid oxidative pathways (Fig. 4). In this study, we will discuss support for these strategies.

FIG. 4.

Possible therapeutic strategies involving lipids to prevent AMD development and progression. Based on previous AMD mouse model studies, we propose 3 therapeutic strategies involving lipids. Left: Targeting pathogenic lipid species in sub-RPE deposits by preventing their accumulation or removing them may reduce their ability to induce an inflammatory response in the sub-RPE milieu, to cause RPE damage, and may impede the development of AMD. Middle: Lipid homeostasis in the RPE is modulated by the actions of various transcription factors such as SREBPs, LXRs, and RXRs as well as autophagy. It is known that aging disrupts the signaling associated with these transcription factors and autophagic processes. Restoring these pathways may serve as valuable therapeutics in preventing AMD. Right: Mitochondria are important regulators of lipid oxidation. With age, mitochondria number and function decline in the RPE that may predispose the eye to AMD. By preserving or augmenting mitochondrial function, the RPE may remain healthy and avoid the dysfunction seen in AMD. LXRs, liver x receptors; RXRs, retinoid X receptors; SREBPs, sterol regulatory element-binding proteins.

Clearance of pathogenic lipid or protein components in Sub-RPE deposits

Testing therapies directed at preventing the formation and/or accumulation of BLamDs in AMD mouse models is a good use of these models with the caveat that these deposits are not completely analogous to pathogenic drusen in AMD,^{21,77,80,102,104,105,109,110,117–119,128,132,224} such that therapies that work in these preclinical models may not necessarily translate well to human clinical trials. In support of this approach, it is important to note that many drusen constituents are found in BLamDs, including complement components,^{21,81,83,105,106,108–110,118,148,224} apolipoproteins,⁸¹ amyloid beta,⁸¹ extracellular membranous debris,^104,109,224 and long-spaced collagen.^{77,102,107,109} Targeting specific components within BLamDs has led to amelioration of AMD-like pathologies in AMD mouse models. For example, aged transgenic APOE mice with targeted replacement of mouse ApoE with human APOE4 develop an AMD-like ocular phenotype, including decreased visual function, CNV, RPE damage, and BLamD accumulation after an 8-week high-fat, cholesterol-enriched (HFC) diet.⁸⁰ APOE4 is associated with decreased risk for AMD in humans but the pathogenicity caused by the E4 allele in aged mice fed a HFC diet is unknown.^59–62

The BLamDs of APOE4∼HFC mice contain complement activated products and amyloid-beta.⁸¹ Targeting amyloid beta with a systemic anti-amyloid-beta immunotherapy prevented amyloid-beta accumulation, sub-RPE complement activation, and AMD-like pathologies, although there was no change in BLamD load.⁸¹ Preventing the accumulation of toxic inflammatory components within drusen-like amyloid beta may be a viable therapeutic approach for AMD. However, a humanized monoclonal antibody against amyloid beta was tested in a clinical trial for the treatment of geographic atrophy but it failed to slow the geography atrophy enlargement in patients,²²⁵ indicating not all therapies assessed in mice are translatable to human diseases. Still, it should be noted that the anti-amyloid antibody, RN6G, which showed efficacy in the mouse and targets the c-terminus of both Ab40 and Ab42 was not the one tested in clinical trials.⁸¹

Similar to amyloid beta, oxidized lipids can be pathogenic to RPE cells.²⁶ A therapeutic strategy for targeting inflammatory lipids in AMD has emerged from studies of AMD mouse models with varying complement factor H (CFH) activity.^20,21,138 CFH is a major AMD susceptibility gene^226–229 that functions as the main soluble regulator of the alternative complement pathway by serving as a cofactor for factor I-mediated proteolytic inactivation of C3b²³⁰ and accelerating the decay of the C3 convertase that is responsible for the intial activation and propagation of the complement cascade.²³¹ The importance of CFH in regulating the formation of the C3 convertase is exemplified by the absence of intact plasma C3 in Cfh knockout (Cfh^−/−) mice due to uncontrolled C3 cleavage.^21,232

In addition to its canonical functions, we have found that CFH can bind and decrease adherence of ApoE- and ApoB-containing lipoproteins to BrM.²¹ To examine the significance of impaired CFH binding to lipoproteins in vivo, C57BL/6J, Cfh heterozygous (Cfh^+/−), and Cfh^−/− mice were aged to ninety weeks and then fed a HFC diet for 8 weeks to exacerbate the subtle AMD-like phenotype seen in aged Cfh^−/− mice.^21,108,109 Consumption of a HFC diet leads to increased circulating lipoproteins in mice.²³³ Both aged Cfh^+/− and Cfh^−/− mice accumulate sub-RPE basal deposits in response to the HFC diet.²¹ Notably, only the aged Cfh^+/− and not the Cfh^−/− null mice fed a HFC diet, developed sustained vision loss and RPE damage.²¹ Aged Cfh^−/− mice may be protected against HFC-induced ocular damage from pathogenic sub-RPE basal deposits because they lack a reservoir of complement components and they display increased expression of membrane-bound complement regulators in the posterior eye, whereas Cfh^+/− mice possess an intact complement system, but express only half the levels of Cfh as wild-type mice.^21,234,235

CFH also acts to regulate RPE-derived lipoprotein accumulations in BrM. Evidence for a unique RPE-derived lipoprotein comes from biochemical assessments of human donor eyes.^23,236 RPE cells possess the machinery required to generate lipoproteins²³⁷ and have been validated by detecting secreted lipoproteins in the media of RPE cell cultures.^236–238 Our recent study of transgenic mice expressing equal amounts of the full-length normal human CFH Y402 versus the AMD-risk-associated CFH H402 variant on a Cfh null background (CFH-Y/0 and CFH-H/H, respectively) revealed a correlation between RPE-derived lipoproteins and pathologies.²⁰ The CFH Y402H polymorphism is one of the most replicated genetic variants associated with AMD risk,^226–229 so these mice were developed to test the in vivo effect of the Y402H CFH risk variant on AMD pathobiology.^20,109 CFH-Y/0 and CFH-H/H mice were aged to 90 weeks on a normal mouse chow diet (ND) and then switched to a HFC diet for 8 weeks to test for an AMD phenotype as aging and the consumption of a HFC diet are sufficient to elicit AMD-like pathology development in Cfh heterozygous mice and APOE4-targeted replacement mice.^21,80 Only the old CFH-H/H mice fed HFC (CFH-H/H∼HFC) developed AMD-like pathologies.²⁰

Quantitation of plasma lipoproteins in these mice revealed decreases in plasma LDLs and its markers, apolipoprotein B100 (ApoB100), and ApoE in aged CFH-H/H∼HFC mice compared with aged CFH-Y/0 mice fed HFC (CFH-Y/0∼HFC), but no change in any other lipoprotein class.²⁰ Strikingly, however, biochemical analyses revealed that changes in eyecup apolipoproteins correlated with the AMD-like phenotype seen in the CFH-H/H∼HFC, where apolipoproteins B48 (ApoB48) and A1 (ApoA-1) are elevated in the RPE/choroid of the aged CFH-H/H∼HFC mice compared with age-matched control CFH-Y/0 after an 8-week HFC diet.²⁰ Thus, we are the first to establish a functional consequence of the Y402H polymorphism in vivo, promoting AMD-like pathology and affecting lipoprotein levels in aged mice.²⁰

The identity of molecular pathways involved in RPE lipoprotein synthesis and secretion are largely unknown, but insight may be gleaned from a dietary intervention study using aged CFH-H/H mice performed in our laboratory. Diets with similar macronutrient composition as the HFC diet previously used, which either had the dietary cholesterol removed (HF) or the fat removed [high cholesterol (HC)], were used to test the role of dietary cholesterol and fat on circulating lipoprotein and visual function in aged CFH-H/H mice. Aged CFH-H/H mice consuming a HFC or HC diet have increased chylomicron (CM)-, VLDL-, and LDL-containing cholesterol compared with aged CFH-H/H mice after a ND or HF diet (Fig. 5A). The area under each lipoprotein curve was calculated and the confirmed levels of these lipoproteins were statistically different between these groups (Fig. 5B). Visual function of aged CFH-H/H mice was assessed by scotopic electroretinography (ERG) after the consumption of an 8-week HF or HC diet.⁸¹ Using another cohort of aged CFH-H/H mice, these mice developed a similar decrease of scotopic ERG b-wave responses after the consumption of an 8-week HFC diet (Fig. 5C), as previously described.²⁰ No change was observed in aged CFH-H/H∼HF mice relative to aged CFH-H/H∼ND mice (Fig. 5D). Notably, aged CFH-H/H∼HC mice developed significantly attenuated ERG b-wave responses compared with aged CFH-H/H∼ND mice (Fig. 5E).

FIG. 5.

Dietary intervention effects on plasma lipoprotein levels and visual function of aged CFH-H/H mice. Male CFH-H/H mice over 90 weeks of age, housed conventionally and maintained on ND (Isopurina 5001; Prolab) were either continued on ND or switched to a HFC (Envigo #88051), HF (Envigo #98232) or HC (Envigo #91342) diet for 8 weeks. All mice were negative for the rd8 mutation. Protocols for FPLC fractionation, cholesterol quantification, ERG, and statistical analysis are described in Landowski et al.²⁰ (A) FPLC fractions of male aged CFH-H/H mice after an 8-week ND, HFC, HF, and HC diet. (B) Averages of the area under the FPLC curve for aged male CFH-H/H mice after an 8-week ND, HFC, HF, and HC diet. Consumption of dietary cholesterol increases CM/VLDL and LDL cholesterol fractions in aged male CFH-H/H mice relative to the ND- and HF-fed groups. (C–E) Visual function in aged male CFH-H/H mice after an 8-week ND, HFC, HF, and HC diet. Analysis of scotopic ERG b-wave responses reveals statistically lower b-wave amplitudes in aged CFH-H/H mice fed a HFC and HC diet compared with ND-fed controls. Data are presented as fitted lines of the average. Mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001. CFH, complement factor H; CM, chylomicron; ERG, electroretinography; FPLC, fast protein liquid chromatography; HC, high cholesterol with no added cocoa butter fat; HDL, high-density lipoprotein; HF, high fat with no added cholesterol; HFC, high-fat, cholesterol-enriched; LDL, low-density lipoprotein; ND, normal mouse chow diet; nd, not detected; VLDL, very-low-density lipoprotein.

The correlation of decreased visual function with consumption of dietary cholesterol in aged CFH-H/H mice fed a HC diet but not when fed a HF diet, supports the notion that therapeutic strategies targeting cholesterol intake through the small intestine (such as ezetimibe) may prevent visual loss in aged CFH-H/H∼HFC mice. Ezetimibe interacts with the Neimann Pick C1-like intracellular cholesterol transporter 1 (NPC1) to prevent cholesterol uptake by intestinal enterocytes and thereby lowering the level of plasma LDLs containing cholesterol.²³⁹ Ongoing studies are examining whether ezetimibe could mimic the effects of dietary modulation in aged CFH-H/H mice and if HFC diet-induced increases in eyecup ApoB48 and ApoA-1 in aged CFH-H/H mice is influenced by dietary cholesterol.

ApoA-1 is a major protein constituent of HDL, a lipoprotein associated with AMD risk.^48–51 In the posterior eye, the major site of interaction between CFH and HDL occurs at heparan sulfation within BrM.^22,240 We postulate that augmenting CFH concentrations or soluble heparan sulfate in the posterior eye may prevent toxic accumulations of lipoproteins such as HDL in BrM and protect against RPE damage and death. In support of this, treatment of BrM explants from human donor eyes with short heparan sulfate oligosaccharides or an ApoA-1 mimetic was sufficient to remove lipoproteins from BrM.²³ In addition, aged nonhuman primates that got an intravitreal injection of an ApoA-1 mimetic had less neutral lipid, esterified cholesterol, and activated complement components in BrM than placebo-treated controls.²⁴¹ Pharmaceutical interventions aimed at lipoprotein binding in BrM may be effective in the treatment of AMD and should be considered in future studies involving AMD mouse models.

Restoration of lipid processing in the RPE and BrM

Restoration of pathways that the RPE cells use to process lipids could be a means to therapeutically target lipid metabolism in AMD. For example, multiple transcription factors, including sterol regulatory element-binding proteins (SREBPs),²⁴² liver X receptors (LXRs),²⁴³ retinoid X receptors (RXRs),²⁴⁴ and peroxisome proliferator-activated receptors (PPARs)²⁴⁵ participate in intracellular lipid homeostasis. The SREBP, LXR, RXR, and PPAR pathways are present in RPE cells^100,123,215 and their importance in lipid homeostasis of the RPE is supported by reports of visual loss and lipid deposition in mice lacking LXR and PPAR β/δ signaling.^100,123

In a study aimed at determining if activating LXRs can prevent AMD-like pathology development, 3-month-old mice with a mutation in apolipoprotein B100 (apob100) that prevents the formation of its alternative splice variant apolipoprotein B48 (apob48) were fed a low-fat diet for 5 months and given GW3965, an LXR agonist.¹⁰⁰ Limiting expression to only ApoB100 in mice increased LDL triglyceride and cholesterol levels, resembling lipoprotein profiles in humans.²⁴⁶ As a consequence of this metabolic change, age-related progression of lipid deposition occurs in the BrM of apob100 mice but no other pathologies were noted in these animals.¹⁰¹ GW3965 treatment improved hypopigmented regions in fundus images, dampened neuroinflammation, and decreased lipid deposition in the apob100 mice after the 5-month low-fat diet.¹⁰⁰ Differences in the ocular phenotypes of the apob100 mice may be explained by the standard mouse chow and low-fat diet consumed by mice in these 2 studies.^100,101 More investigations of LXR agonists and other agents modulating transcription factors in other AMD mouse models are needed to confirm the promising results after treating apob100 mice with GW3965.

Another pathway critical for lipid metabolism within the RPE is autophagy, a conserved catabolic pathway induced under cellular stresses.²⁴⁷ RPE cells depend on autophagy for the daily breakdown of photoreceptor outer segments.¹⁴⁴ Autophagy is increased in RPE cells of aged non-AMD eyes, but decreased in RPE cells of human donor eyes diagnosed with AMD.²⁴⁸ Mice with decreased autophagy such as microtubule-associated protein 1 light chain 3 B (LC3B) knockout, lysosomal-associated membrane protein 2 (Lamp2) knockout,¹¹⁴ and RPE-specific RB1-inducible coiled-coil 1 (Rb1cc1) knockout mice have lipid accumulations within the RPE.^142,144 As a consequence, these mouse lines develop visual loss, migration of immune cells into the subretinal space, and subRPE deposits.^114,142,144 Studies utilizing RPE cell cultures identified a synthetic lignan secoisolariciresinol diglucoside, LGM2605,²⁴⁹ and flubendazole²⁵⁰ as inducers of autophagy that were able to reduce intracellular RPE lipid levels. Testing these therapies in mice with increased RPE lipid accumulation like RPE-specific ABCA1 and ABCG1 knockout mice⁶⁶ could be an effective means testing if targeting autophagy may be a therapy for AMD.

Preservation of lipid oxidative pathways

Another possible intervention is to sustain the lipid oxidative function of RPE cells through preservation of mitochondrial health. Mitochondria are critical organelles required for the breakdown of fatty acids for ATP production through beta-oxidation²⁵¹ and regulation of reactive oxygen species that, when in excess, leads to lipid peroxidation.²⁵² With age, mitochondrial function declines and impacts retinal homeostasis.³³ Multiple AMD mouse models are based on mitochondrial dysfunction, including the Tmem135 transgenic (Tmem135 TG)¹²¹ and superoxide dismutase 2 knockout (Sod2^−/−).^117,118,253 It is possible that other mouse models may have mitochondrial impairment such as the DICER1-deficient mice.^120,152,153 Not only is there a consequence of an accumulation of Alu elements in DICER1-deficient mice, they also have decreased mitochondrial genome-encoded small RNAs and consequent reduced mitochondrial gene expression.²⁵⁴ RPE degeneration is a common feature in these AMD mouse models.^{117,118,120,121,153,253,254} The RPE degeneration in these models phenocopies mice with RPE-specific ablation of mitochondrial transcription factor A (Tfam)²⁵⁵ and global knockout of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (Pgc1α),²⁵⁶ proteins required for mitochondrial biogenesis.

The RPE-specific Tfam knockout mice have increased activation of the mammalian target of rapamycin (mTOR) pathway,²⁵⁵ a pathway crucial for nutrient sensing and homeostasis.²⁵⁷ Abnormal mTOR activation can cause RPE degeneration²⁵⁸ but inhibiting mTOR in mice with RPE-specific ablation of TFAM or in mice treated with sodium iodate, alleviates RPE pathologies.²⁵⁵ Furthermore, activation of mTOR signaling through ablation of tuberous sclerosis complex 1 (Tsc1) in rod photoreceptors leads to RPE abnormalities.¹²² Conversely, activating mTOR in mouse models of retinitis pigmentosa is sufficient to reduce cone photoreceptor cell death,^259–262 whereas inhibiting mTOR through rapamycin can decrease retinal vascularization and lead to hypoxia.²⁶³

One important aspect of the mTOR pathway is its ability to coordinate protein synthesis.²⁶⁴ One study mimicked inhibition of the mTOR pathway by administering anisomycin, which curbs eukaryotic protein synthesis, to rd16 and wild-type C57BL/6J mice.²⁶⁵ The rd16 mice had accelerated retinal degeneration, whereas wild-type mice developed retinal pathologies after anisomycin treatment.²⁶⁵ Restoring mitochondrial function, rather than targeting the mTOR pathway, may be a safer avenue for an AMD therapy, as it is less likely to damage other nearby ocular tissues.

Lipid Metabolism-Targeting Therapies Tested in Preclinical AMD Mouse Models

A fraction of the preclinical AMD mouse models (Fig. 2 and Table 1) has been utilized to evaluate lipid metabolism-targeting therapies. These therapies include desipramine, TO901316, docosahexaenoic acid (DHA), apolipoprotein mimetics, and statins (Table 2). In this study, we present published studies on these therapies as they offer perspectives on potential treatments for AMD.

Table 2.

Lipid Therapies Tested in Age-Related Macular Degeneration Mouse Models

AMD mouse model	Retinal pathologies observed in model	Pharmacological treatment	Mode of action	Observed effects due to pharmacological treatment	References
5-month-oldAbca4⁻^/−	• Delayed dark adaption• Elevated PE in outer segments• Increased A2E in RPE• RPE vacuolization• Increased retinal all-trans-RAL after light exposure• Increased A2E and lipofuscin granules in RPE• Cholesterol and ceramide accumulation in RPE• Increased early endosome number and size• Augmented complement activation	3 Intraperitoneal injections of desipramine for 4 weeks	Inhibitor of ASMase that prevents ceramide production	• Decreased early endosome volume and number in RPE• Prevented C3a activation and signaling in RPE	²⁷⁴
5-month-oldAbca4⁻^/−		3 Intraperitoneal injections of TO901316 for 4 weeks	LXR agonist	• Decreased early endosome volume and number in RPE	²⁷⁴
Various aged Elovl4^mut/−	• Presence of fundus abnormalities• Photoreceptor degeneration• Decreased scotopic ERG a-wave and b-wave• Increased lipofuscin and A2E in RPE• Loss of synapses• Neuroinflammation• Extensive inner retinal remodeling	Dietary DHA supplementation for various lengths of time	C22:6n-3 fatty acid that has anti-inflammatory, anti-angiogenic and antiapoptotic effects	• Preservation of ERG c-wave in 6-month-treated mutant mice• Preservation of cone function in 12-month-treated mutant mice• Decreased A2E levels in 18-month-treated mutant mice	^280a
10-month-old Apoe^−/−	• Increased implicit time for scotopic b-wave• Decreased ERG oscillatory potential amplitudes• Decreased ONL nuclei number• Thicker BrM• Increased retinal cholesterol	Single intravitreal injection of 4F	APOA1 mimetic with anti-inflammatory and antiatherogenic properties	• Decreased BrM thickness• Decreased esterfied cholesterol deposition in BrM	²⁹⁰
Intravenous injection of sodium iodate in 6-week-old C57BL/6J	• RPE degeneration• Photoreceptor degeneration• Neuroinflammation	Dietary HM-10/10 treatment for 3 weeks	APOE/APOJ mimetic possessing antioxidant and anti-inflammatory properties	• Decreased retinal thinning• Prevented photoreceptor degeneration• Reduced cleaved caspase-3 in retina	²⁹¹
6-week-old C57Bl/6J∼30-week HF diet	• Presence of fundus abnormalities• Decrease of scotopic ERG a-wave and b-wave• Accumulation of lipids• RPE vacuolization• Deposition of heterogenous debris below the RPE• Thickened BrM thickness	Dietary simvastatin treatment for 30 weeks	Inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase that lowers endogenous cholesterol synthesis	• Decreased prevalance of fundus abnormalities• No accumulation of lipids• Decreased RPE vacuolization• No deposition of heterogenous Debris below the RPE• Decreased BrM thickness	⁸²

No beneficial effect was seen in a different study using a different Elovl^mut/+ mouse after a dietary DHA supplementation.²⁷⁹

ApoA1, apolipoprotein A-I; APOJ, apolipoprotein J; ASMase, acid sphingomyelinase; DHA, docosahexaenoic acid; PE, phosphatidylethanolamine.

Despiramine and TO901316

ABCA4 is a member of the ABC transporter family found on rod and cone photoreceptor outer segments.^266–268 It is responsible for the clearance of all-trans-retinal from the disc membranes after phototransduction through the transport of N-retinylidene-phosphatidylethanolamine (PE), a product of the reaction of all-trans-retinal with PE.²⁶⁹ If N-retinylidene-PE is not removed from the disc membranes, N-retinylidene-PE can react with all-trans-retinal to form toxic accumulations of N-retinylidene-N-retinylethanolamine (A2E) and other bisretinoids in the RPE.²⁶⁹ Ablation of the murine Abca4 gene recapitulates STGD1-like phenotypes in mice, including age-dependent vision loss, delayed dark adaptation, increased retinal PE, deposition of A2E in the RPE, and additional toxic phototransduction byproducts.^166,270,271

The spatial distribution of lipids in Abca4^−/− RPE detected through a combination of matrix-assisted laser desorption ionization and Fourier transform ion cyclotron resonance imaging mass spectrometry reveals increased bis(monoacylglycerol)phosphate (BMP) lipid species.²⁷² BMP lipid species are commonly observed in endosomal/lysosomal storage diseases and regulate cholesterol levels in endosomes.²⁷³ Kaur et al. reported enlarged early endosomes in the RPE of Abca4^−/− mice that allow more extracellular complement component 3 (C3) intake and activation of the C3-proteolytic cleavage product, C3a.²⁷⁴ Inhibiting acid sphingomyelinase with desipramine can decrease the size of early endosomes and prevent C3a activation in Abca4^−/− RPE.²⁷⁴ Promoting cholesterol efflux with a LXR agonist, TO901316, can mimic the effects of desipramine on early endosome size in the Abca4^−/− RPE.²⁷⁴ Decreasing the size of early endosomes in the RPE may be a viable therapeutic strategy for AMD, although more phenotypic work is needed to determine the functional effects of desipramine and TO901316 on the RPE in Abca4^−/− mice and other models with RPE pathologies.

DHA treatment

Mutations in ELOVL4 are known to cause mislocalization and aggregation of ELOVL4 from the endoplasmic reticulum to other organelles in photoreceptors leading to STGD3.^275,276 ELOVL4 is a fatty acid elongase that preferentially uses eicosapentaenoic acid (EPA) as a substrate to generate very-long-chain polyunsaturated fatty acids (VLC-PUFAs).²⁷⁷ To model STGD3, researchers identified a 5 base pair deletion in ELOVL4 of a STGD3 patient¹⁹⁵ and generated transgenic mice expressing ELOVL4 with either the same 5 base pair deletion in human ELOVL4¹⁷⁵ or mouse Elovl4.¹⁷⁶ Regarding the efficacy of omega-3 VLC-PUFAs as a treatment for STGD3, a recent long-term clinical study testing diets supplemented with omega-3 VLC-PUFAs, such as EPA and DHA, in STGD3 patients found no changes in the disease progression.²⁷⁸ Dietary DHA supplementation was also tested in mice with a mutant ELOVL4 allele but these studies yielded conflicting results.^279,280 This could be due to the differential effect of the mutant ELOVL4 and Elovl4 allele on VLC-PUFA synthesis in the mouse retina. Only the mice with a mutant Elovl4 allele displayed a 50% reduction in retinal VLC-PUFAs.¹⁷⁴ Further studies have confirmed the essential role of ELOVL4 in retinal VLC-PUFA synthesis by ablating Elovl4 in cone and rod photoreceptors.²⁸¹ Therefore, it would be important to test if DHA supplementation could be a viable therapeutic strategy in other mouse models of disrupted ELOVL4 function. This is especially important since DHA was added to the AREDS2 formulation and could explain why there was no reduction in AMD risk with the AREDS2 formulation.²⁸²

Apolipoprotein mimetics

Repurposing lipid-lowering pharmacologic agents that have been effective in other diseases may be beneficial to treat AMD. For example, a major risk factor for cardiovascular disease is increased LDL and decreased HDL.²⁸³ The opposite has been shown for AMD where increased HDL has emerged as a major risk factor.^48–51 One possible strategy to treat cardiovascular disease is to promote HDL function through ApoA1 mimetics. HDL particles that harbor ApoA1 are critical for reverse cholesterol transport, which removes lipids from cells and provides anti-inflammatory protection.²⁸⁴ ApoA1 mimetics vary in peptide length and promote reverse cholesterol transport and are anti-inflammatory.²⁸⁵ However, the effectiveness of ApoA1 mimetics as a cardiovascular disease treatment has been variable with some reports indicating no beneficial changes in patients.^286–288 and others describing increased HDL function in patients.²⁸⁹

To determine if ApoA1 mimetics could be effective against AMD, 10-month-old Apoe^−/− mice were treated with a single intravitreal injection of the ApoA1 mimetic, 4F, and assessed 30 days postinjection for changes in BrM.²⁹⁰ 4F mimetic treatment sufficiently lowered esterified cholesterol and prevented ultrastructural changes in Apoe^−/− mice.²⁹⁰ In addition, based on our findings of increased ApoA1 in CFH-H/H∼HFC mice, we tested the ApoA1 mimetic 5A for 8 weeks in CFH-H/H∼HFC and were able to block HFC diet-induced changes in their plasma HDL proteome.²³ We are currently testing whether the ApoA1 5A mimetic treatment correlate with changes in visual function that occur in both Apoe^−/− and CFH-H/H after a HF, cholesterol-enriched diet in these genotypes.^20,78

Other apolipoprotein mimetics have been tested in preclinical AMD mouse models such as the hybrid ApoE and apolipoprotein J (ApoJ) mimetic, HM-10/10 in the intravenous sodium iodate treatment model of geographic atrophy.²⁹¹ Treatment of C57BL/6J mice with a diet chow containing HM-10/10 after induction of sodium iodate-induced RPE injury was sufficient to partially protect the retina and prevent caspase-3 cleavage in RPE cells.²⁹¹ Since recruitment of immune cells is a major consequence of sodium iodate injury in the retina,²⁹² it would be interesting to investigate whether HM-10/10 can reduce sodium iodate-induced immune cell recruitment to the retina as this frequently occurs in AMD mouse models and AMD-afflicted eyes.²⁹³

Statins

Pharmaceuticals that could work for AMD and revolutionized the treatment of cardiovascular disease are statins, a class of lipid-lowering agents inhibiting the activity of 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase to increase the uptake of LDLs and decrease plasma cholesterol.²⁹⁴ A recent pilot study, which was randomized and placebo controlled, showed that taking simvastatin (40 mg/day) slowed progression of nonadvanced AMD especially for those with the CFH Y402H genotype.²⁹⁵ Additionally, an open-label prospective pilot multicenter clinical trial found that a high dose of statins given to 26 patients resolved drusenoid pigment epithelial detachments and improved visual acuity without any progression to RPE atrophy or CNV formation.²⁹⁶ However, the potency of statins as a treatment for AMD is controversial as evident by varying conclusions of case/control cross-sectional studies where statin usage had a beneficial,^297–299 worsened,^300,301 or no effect in AMD.^302–314

To determine efficacy in an animal model, female C57BL/6 mice were orally treated with simvastatin, atorvastatin, rosuvastatin, and pravastatin at similar concentrations where simvastatin had the highest accumulation in the retina.³¹⁵ Oral gavage of simvastatin led to a 24% reduction in retinal cholesterol content,³¹⁵ suggesting simvastatin can inhibit cholesterol synthesis in the retina. C57BL/6 mice fed a HF diet for 30 weeks were treated concurrently with simvastatin to evaluate the effect of simvastatin on retinal pathology development.⁸² Simvastatin treatment decreased fundus abnormalities and BrM thickness, but did not significantly ameliorate visual function induced by the 30-week HF diet regime.⁸² The absence of a visual function change could be due to an upregulation of cluster of differentiation 36 (CD36), an oxidized LDL receptor critical for ingestion of photoreceptor outer segments,³¹⁶ in the mouse retina.³¹⁵ Intake of oxidized LDL in the posterior eye can lead to increased expression of genes regulating oxidative stress, inflammation, and angiogenesis,^317–320 as well as an increase in the number of apoptotic RPE cells.^317,321,322 Further analysis of the C57BL/6∼HF mouse retina may answer whether statins could work as a treatment for AMD.

Conclusion

AMD is a debilitating blindness with limited therapeutic options but there is hope of new treatments for this age-dependent retinal disease in the future. Over the years, implications of lipid metabolic dysregulation as a key pathobiological mechanism in AMD have emerged. We presented an overview of preclinical AMD mouse models that allow for the establishment of causative relationships with lipids and AMD-like pathology development. The use of these AMD mouse models may lead to worthy lipid-targeting candidate therapies for clinical trials to prevent the blindness caused by AMD.

Footnotes

Acknowledgments

The authors thank Dan Stamer, Mikael Klingeborn, and Daniel Grigsby for their intellectual feedback and advice.

Author Disclosure Statement

No competing financial interests exist.

Funding Information

This work was supported by NEI, the National Institutes of Health, Grants R01 EY026161, EY031748 (to C.B.R.), and P30 EY005722 (to Duke Eye Center); an Edward N. and Della L. Thome Memorial Foundation Award in Age-Related Macular Degeneration Research (to C.B.R.); a Research to Prevent Blindness/International Retinal Research Foundation Catalyst award for Innovative Research Approaches for AMD (to C.B.R.); and an unrestricted grant from Research to Prevent Blindness (to the Duke Eye Center). M.L. is supported by a NIH T32EY027721 and F32EY032766 grant.

References

Wong

W.L.

, Su

, Li

, Cheung

C.M.

, Klein

, Cheng

C.Y.

, and Wong

T.Y.

Global prevalence of age-related macular degeneration and disease burden projection for 2020 and 2040: a systematic review and meta-analysis. Lancet Glob. Health. 2:e106–e116, 2014.

Jager

R.D.

, Mieler

W.F.

, and Miller

J.W.

Age-related macular degeneration. N. Engl. J. Med. 358:2606–2617, 2008.

Klein

, Klein

B.E.

, and Linton

K.L.

Prevalence of age-related maculopathy. The Beaver Dam Eye Study. Ophthalmology. 99:933–943, 1992.

Vingerling

J.R.

, Dielemans

, Hofman

, Grobbee

D.E.

, Hijmering

, Kramer

C.F.

, and de Jong

P.T.

The prevalence of age-related maculopathy in the Rotterdam Study. Ophthalmology. 102:205–210, 1995.

Miller

J.W.

Age-related macular degeneration revisited—piecing the puzzle: the LXIX Edward Jackson memorial lecture. Am. J. Ophthalmol. 155:1–35, 2013.

Bowes Rickman

, Farsiu

, Toth

C.A.

, and Klingeborn

Dry age-related macular degeneration: mechanisms, therapeutic targets, and imaging. Invest. Ophthalmol. Vis. Sci. 54:ORSF68–ORSF80, 2013.

Owsley

, McGwin

, Clark

M.E.

, Jackson

G.R.

, Callahan

M.A.

, Kline

L.B.

, Witherspoon

C.D.

, and Curcio

C.A.

Delayed rod-mediated dark adaptation is a functional biomarker for incident early age-related macular degeneration. Ophthalmology. 123:344–351, 2016.

Ferris

F.L.

, Davis

M.D.

, Clemons

T.E.

, Lee

L.Y.

, Chew

E.Y.

, Lindblad

A.S.

, Milton

R.C.

, Bressler

S.B.

, Klein

, and Age-Related Eye Disease Study (AREDS) Research

Group.

A simplified severity scale for age-related macular degeneration: AREDS Report No. 18. Arch. Ophthalmol. 123:1570–1574, 2005.

Farazdaghi

M.K.

, and Ebrahimi

K.B.

Role of the choroid in age-related macular degeneration: a current review. J. Ophthalmic Vis. Res. 14:78–87, 2019.

10.

Bonilha

V.L.

Age and disease-related structural changes in the retinal pigment epithelium. Clin. Ophthalmol. 2:413–424, 2008.

11.

Miller

J.W.

Age-related macular degeneration revisited—piecing the puzzle: the LXIX Edward Jackson Memorial Lecture. Am. J. Ophthalmol. 155:48, 2013.

12.

Yazdi

M.H.

, Faramarzi

M.A.

, Nikfar

, Falavarjani

K.G.

, and Abdollahi

Ranibizumab and aflibercept for the treatment of wet age-related macular degeneration. Expert Opin. Biol. Ther. 15:1349–1358, 2015.

13.

, Peng

R.S.

, Xu

, Li

Y.H.

, Shi

, Wang

, and Yu

Intravitreal anti-VEGF injections for treating wet age-related macular degeneration: a systematic review and meta-analysis. Drug Des. Devel. Ther. 9:5397–5405, 2015.

14.

Yang

, Zhao

, and Sun

Resistance to anti-VEGF therapy in neovascular age-related macular degeneration: a comprehensive review. Drug Des. Devel. Ther. 10:1857–1867, 2016.

15.

Gemenetzi

, Lotery

A.J.

, and Patel

P.J.

Risk of geographic atrophy in age-related macular degeneration patients treated with intravitreal anti-VEGF agents. Eye (Lond). 31:1–9, 2017.

16.

Age-Related Eye Disease Study Research Group. A randomized, placebo-controlled, clinical trial of high-dose supplementation with vitamins C and E, beta carotene, and zinc for age-related macular degeneration and vision loss: AREDS report no. 8. Arch. Ophthalmol. 119:1417–1436, 2001.

17.

Aronow

M.E.

, and Chew

E.Y.

Age-related eye disease study 2: perspectives, recommendations, and unanswered questions. Curr. Opin. Ophthalmol. 25:186–190, 2014.

18.

Fritsche

L.G.

, Fariss

R.N.

, Stambolian

, Abecasis

G.R.

, Curcio

C.A.

, and Swaroop

Age-related macular degeneration: genetics and biology coming together. Annu. Rev. Genomics Hum. Genet. 15:151–171, 2014.

19.

Anderson

D.H.

, Radeke

M.J.

, Gallo

N.B.

, Chapin

E.A.

, Johnson

P.T.

, Curletti

C.R.

, Hancox

L.S.

, Hu

, Ebright

J.N.

, Malek

, Hauser

M.A.

, Rickman

C.B.

, Bok

, Hageman

G.S.

, and Johnson

L.V.

The pivotal role of the complement system in aging and age-related macular degeneration: hypothesis re-visited. Prog. Retin. Eye Res. 29:95–112, 2010.

20.

Landowski

, Kelly

, Klingeborn

, Groelle

, Ding

J.D.

, Grigsby

, and Bowes Rickman

Human complement factor H Y402H polymorphism causes an age-related macular degeneration phenotype and lipoprotein dysregulation in mice. Proc. Natl. Acad. Sci. U. S. A. 116:3703–3711, 2019.

21.

Toomey

C.B.

, Kelly

, Saban

D.R.

, and Bowes Rickman

Regulation of age-related macular degeneration-like pathology by complement factor H. Proc. Natl. Acad. Sci. U. S. A. 112:E3040–E3049, 2015.

22.

Toomey

C.B.

, Johnson

L.V.

, and Bowes Rickman

Complement factor H in AMD: bridging genetic associations and pathobiology. Prog. Retin. Eye Res. 62:38–57, 2018.

23.

Kelly

U.L.

, Grigsby

, Cady

M.A.

, Landowski

, Skiba

N.P.

, Liu

, Remaley

A.T.

, Klingeborn

, and Bowes Rickman

High density lipoproteins are a potential therapeutic target for age-related macular degeneration. J. Biol. Chem. 295: 13601–13616, 2020.

24.

Pikuleva

I.A.

, and Curcio

C.A.

Cholesterol in the retina: the best is yet to come. Prog. Retin. Eye Res. 41:64–89, 2014.

25.

van Leeuwen

E.M.

, Emri

, Merle

B.M.J.

, Colijn

J.M.

, Kersten

, Cougnard-Gregoire

, Dammeier

, Meester-Smoor

, Pool

F.M.

, de Jong

E.K.

, Delcourt

, Rodrigez-Bocanegra

, Biarnés

, Luthert

P.J.

, Ueffing

, Klaver

C.C.W.

, Nogoceke

, den Hollander

A.I.

, and Lengyel

A new perspective on lipid research in age-related macular degeneration. Prog. Retin. Eye Res. 67:56–86, 2018.

26.

Jun

, Datta

, Wang

, Pegany

, Cano

, and Handa

J.T.

The impact of lipids, lipid oxidation, and inflammation on AMD, and the potential role of miRNAs on lipid metabolism in the RPE. Exp. Eye Res. 181:346–355, 2019.

27.

Ramachandra Rao

, and Fliesler

S.J.

Cholesterol homeostasis in the vertebrate retina: biology and pathobiology. J. Lipid Res. 62:100057, 2021.

28.

Pennesi

M.E.

, Neuringer

, and Courtney

R.J.

Animal models of age related macular degeneration. Mol. Aspects Med. 33:487–509, 2012.

29.

Chew

E.Y.

, Clemons

T.E.

, Agron

, Sperduto

R.D.

, Sangiovanni

J.P.

, Davis

M.D.

, Ferris

F.L.

, 3rd, and Age-Related Eye Disease Study Research

Group.

Ten-year follow-up of age-related macular degeneration in the age-related eye disease study: AREDS report no. 36. JAMA Ophthalmol. 132:272–277, 2014.

30.

Jonasson

, Fisher

D.E.

, Eiriksdottir

, Sigurdsson

, Klein

, Launer

L.J.

, Harris

, Gudnason

, and Cotch

M.F.

Five-year incidence, progression, and risk factors for age-related macular degeneration: the age, gene/environment susceptibility study. Ophthalmology. 121:1766–1772, 2014.

31.

Luu

, and Palczewski

Human aging and disease: lessons from age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 115:2866–2872, 2018.

32.

van Leeuwen

, Klaver

C.C.

, Vingerling

J.R.

, Hofman

, and de Jong

P.T.

The risk and natural course of age-related maculopathy: follow-up at 6 1/2 years in the Rotterdam study. Arch. Ophthalmol. 121:519–526, 2003.

33.

Eells

J.T.

Mitochondrial dysfunction in the aging retina. Biology (Basel). 8: 31, 2019.

34.

Curcio

C.A.

, Johnson

, Rudolf

, and Huang

J.D.

The oil spill in ageing Bruch membrane. Br. J. Ophthalmol. 95:1638–1645, 2011.

35.

Holz

F.G.

, Sheraidah

, Pauleikhoff

, and Bird

A.C.

Analysis of lipid deposits extracted from human macular and peripheral Bruch's membrane. Arch. Ophthalmol. 112:402–406, 1994.

36.

Pauleikhoff

, Harper

C.A.

, Marshall

, and Bird

A.C.

Aging changes in Bruch's membrane. A histochemical and morphologic study. Ophthalmology. 97:171–178, 1990.

37.

Anderson

D.H.

, Ozaki

, Nealon

, Neitz

, Mullins

R.F.

, Hageman

G.S.

, and Johnson

L.V.

Local cellular sources of apolipoprotein E in the human retina and retinal pigmented epithelium: implications for the process of drusen formation. Am. J. Ophthalmol. 131:767–781, 2001.

38.

Mullins

R.F.

, Russell

S.R.

, Anderson

D.H.

, and Hageman

G.S.

Drusen associated with aging and age-related macular degeneration contain proteins common to extracellular deposits associated with atherosclerosis, elastosis, amyloidosis, and dense deposit disease. FASEB J. 14:835–846, 2000.

39.

Wang

, Clark

M.E.

, Crossman

D.K.

, Kojima

, Messinger

J.D.

, Mobley

J.A.

, and Curcio

C.A.

Abundant lipid and protein components of drusen. PLoS One. 5:e10329, 2010.

40.

C.M.

, Clark

M.E.

, Chimento

M.F.

, and Curcio

C.A.

Apolipoprotein localization in isolated drusen and retinal apolipoprotein gene expression. Invest. Ophthalmol. Vis. Sci. 47:3119–3128, 2006.

41.

Malek

, Li

C.M.

, Guidry

, Medeiros

N.E.

, and Curcio

C.A.

Apolipoprotein B in cholesterol-containing drusen and basal deposits of human eyes with age-related maculopathy. Am. J. Pathol. 162:413–425, 2003.

42.

Huang

, and Mahley

R.W.

Apolipoprotein E: structure and function in lipid metabolism, neurobiology, and Alzheimer's diseases. Neurobiol Dis. 72 Pt A:3–12, 2014.

43.

Rudolf

, Seckerdieck

, Grisanti

, and Curcio

C.A.

Internal structure consistent with remodelling in very small drusen, revealed by filipin histochemistry for esterified cholesterol. Br. J. Ophthalmol. 98:698–702, 2014.

44.

C.M.

, Clark

M.E.

, Rudolf

, and Curcio

C.A.

Distribution and composition of esterified and unesterified cholesterol in extra-macular drusen. Exp. Eye Res. 85:192–201, 2007.

45.

Crabb

J.W.

, Miyagi

, Gu

, Shadrach

, West

K.A.

, Sakaguchi

, Kamei

, Hasan

, Yan

, Rayborn

M.E.

, Salomon

R.G.

, and Hollyfield

J.G.

Drusen proteome analysis: an approach to the etiology of age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 99:14682–14687, 2002.

46.

Rozing

M.P.

, Durhuus

J.A.

, Krogh Nielsen

, Subhi

, Kirkwood

T.B.

, Westendorp

R.G.

, and Sørensen

T.L.

Age-related macular degeneration: a two-level model hypothesis. Prog. Retin. Eye Res. 76:100825, 2020.

47.

Cankova

, Huang

J.D.

, Kruth

H.S.

, and Johnson

Passage of low-density lipoproteins through Bruch's membrane and choroid. Exp. Eye Res. 93:947–955, 2011.

48.

Saunier

, Merle

B.M.J.

, Delyfer

M.N.

, Cougnard-Grégoire

, Rougier

M.B.

, Amouyel

, Lambert

J.C.

, Dartigues

J.F.

, Korobelnik

J.F.

, and Delcourt

Incidence of and risk factors associated with age-related macular degeneration: four-year follow-up from the ALIENOR Study. JAMA Ophthalmol. 136:473–481, 2018.

49.

Fan

, Maranville

J.C.

, Fritsche

, Sim

, Cheung

C.M.G.

, Chen

L.J.

, Gorski

, Yamashiro

, Ahn

, Laude

, Dorajoo

, Lim

T.H.

, Teo

Y.Y.

, Blaustein

R.O.

, Yoshimura

, Park

K.H.

, Pang

C.P.

, Tai

E.S.

, Khor

C.C.

, Wong

T.Y.

, Runz

, and Cheng

C.Y.

HDL-cholesterol levels and risk of age-related macular degeneration: a multiethnic genetic study using Mendelian randomization. Int. J. Epidemiol. 46:1891–1902, 2017.

50.

Cheung

C.M.G.

, Gan

, Fan

, Chee

M.L.

, Apte

R.S.

, Khor

C.C.

, Yeo

, Mathur

, Cheng

C.Y.

, Wong

T.Y.

, and Tai

E.S.

Plasma lipoprotein subfraction concentrations are associated with lipid metabolism and age-related macular degeneration. J. Lipid Res. 58:1785–1796, 2017.

51.

Colijn

J.M.

, den Hollander

A.I.

, Demirkan

, Cougnard-Gregoire

, Verzijden

, Kersten

, Meester-Smoor

M.A.

, Merle

B.M.J.

, Papageorgiou

, Ahmad

, Mulder

M.T.

, Costa

M.A.

, Benlian

, Bertelsen

, Bron

A.M.

, Claes

, Creuzot-Garcher

, Erke

M.G.

, Fauser

, Foster

P.J.

, Hammond

C.J.

, Hense

H.W.

, Hoyng

C.B.

, Khawaja

A.P.

, Korobelnik

J.F.

, Piermarocchi

, Segato

, Silva

, Souied

E.H.

, Williams

K.M.

, van Duijn

C.M.

, Delcourt

, Klaver

C.C.W.

, European Eye Epidemiology

, and EYE-RISK

Consortium.

Increased high-density lipoprotein levels associated with age-related macular degeneration: evidence from the EYE-RISK and European Eye Epidemiology Consortia. Ophthalmology. 126:393–406, 2019.

52.

Acar, İ., Lores-Motta

, Colijn

J.M.

, Meester-Smoor

M.A.

, Verzijden

, Cougnard-Gregoire

, Ajana

, Merle

B.M.J.

, de Breuk

, Heesterbeek

T.J.

, van den Akker

, Daha

M.R.

, Claes

, Pauleikhoff

, Hense

H.W.

, van Duijn

C.M.

, Fauser

, Hoyng

C.B.

, Delcourt

, Klaver

C.C.W.

, Galesloot

T.E.

, den Hollander

A.I.

, and EYE-RISK

Consortium.

integrating metabolomics, genomics, and disease pathways in age-related macular degeneration: the EYE-RISK Consortium. Ophthalmology. 127:1693–1709, 2020.

53.

Colak

, Kosanović-Jaković

, Zorić

, Radosavljević

, Stanković

, and Majkić-Singh

The association of lipoprotein parameters and C-reactive protein in patients with age-related macular degeneration. Ophthalmic Res. 46:125–132, 2011.

54.

Yang

, Skiba

N.P.

, Tewkesbury

G.M.

, Treboschi

V.M.

, Baciu

, and Jaffe

G.J.

Complement-mediated regulation of apolipoprotein e in cultured human RPE cells. Invest. Ophthalmol. Vis. Sci. 58:3073–3085, 2017.

55.

Ishida

B.Y.

, Bailey

K.R.

, Duncan

K.G.

, Chalkley

R.J.

, Burlingame

A.L.

, Kane

J.P.

, and Schwartz

D.M.

Regulated expression of apolipoprotein E by human retinal pigment epithelial cells. J. Lipid Res. 45:263–271, 2004.

56.

Johnson

L.V.

, Forest

D.L.

, Banna

C.D.

, Radeke

C.M.

, Maloney

M.A.

, Hu

, Spencer

C.N.

, Walker

A.M.

, Tsie

M.S.

, Bok

, Radeke

M.J.

, and Anderson

D.H.

Cell culture model that mimics drusen formation and triggers complement activation associated with age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 108:18277–18282, 2011.

57.

Fritsche

L.G.

, Igl

, Bailey

J.N.

, Grassmann

, Sengupta

, Bragg-Gresham

J.L.

, Burdon

K.P.

, Hebbring

S.J.

, Wen

, Gorski

, Kim

I.K.

, Cho

, Zack

, Souied

, Scholl

H.P.

, Bala

, Lee

K.E.

, Hunter

D.J.

, Sardell

R.J.

, Mitchell

, Merriam

J.E.

, Cipriani

, Hoffman

J.D.

, Schick

, Lechanteur

Y.T.

, Guymer

R.H.

, Johnson

M.P.

, Jiang

, Stanton

C.M.

, Buitendijk

G.H.

, Zhan

, Kwong

A.M.

, Boleda

, Brooks

, Gieser

, Ratnapriya

, Branham

K.E.

, Foerster

J.R.

, Heckenlively

J.R.

, Othman

M.I.

, Vote

B.J.

, Liang

H.H.

, Souzeau

, McAllister

I.L.

, Isaacs

, Hall

, Lake

, Mackey

D.A.

, Constable

I.J.

, Craig

J.E.

, Kitchner

T.E.

, Yang

, Su

, Luo

, Chen

, Ouyang

, Flagg

, Lin

, Mao

, Ferreyra

, Stark

, von Strachwitz

C.N.

, Wolf

, Brandl

, Rudolph

, Olden

, Morrison

M.A.

, Morgan

D.J.

, Schu

, Ahn

, Silvestri

, Tsironi

E.E.

, Park

K.H.

, Farrer

L.A.

, Orlin

, Brucker

, Li

, Curcio

C.A.

, Mohand-Said

, Sahel

J.A.

, Audo

, Benchaboune

, Cree

A.J.

, Rennie

C.A.

, Goverdhan

S.V.

, Grunin

, Hagbi-Levi

, Campochiaro

, Katsanis

, Holz

F.G.

, Blond

, Blanche

, Deleuze

J.F.

, Igo

R.P.

Jr ., Truitt

, Peachey

N.S.

, Meuer

S.M.

, Myers

C.E.

, Moore

E.L.

, Klein

, Hauser

M.A.

, Postel

E.A.

, Courtenay

M.D.

, Schwartz

S.G.

, Kovach

J.L.

, Scott

W.K.

, Liew

, Tan

A.G.

, Gopinath

, Merriam

J.C.

, Smith

R.T.

, Khan

J.C.

, Shahid

, Moore

A.T.

, McGrath

J.A.

, Laux

, Brantley

M.A.

Jr ., Agarwal

, Ersoy

, Caramoy

, Langmann

, Saksens

N.T.

, de Jong

E.K.

, Hoyng

C.B.

, Cain

M.S.

, Richardson

A.J.

, Martin

T.M.

, Blangero

, Weeks

D.E.

, Dhillon

, van Duijn

C.M.

, Doheny

K.F.

, Romm

, Klaver

C.C.

, Hayward

, Gorin

M.B.

, Klein

M.L.

, Baird

P.N.

, den Hollander

A.I.

, Fauser

, Yates

J.R.

, Allikmets

, Wang

J.J.

, Schaumberg

D.A.

, Klein

B.E.

, Hagstrom

S.A.

, Chowers

, Lotery

A.J.

, Leveillard

, Zhang

, Brilliant

M.H.

, Hewitt

A.W.

, Swaroop

, Chew

E.Y.

, Pericak-Vance

M.A.

, DeAngelis

, Stambolian

, Haines

J.L.

, Iyengar

S.K.

, Weber

B.H.

, Abecasis

G.R.

, and Heid

I.M.

A large genome-wide association study of age-related macular degeneration highlights contributions of rare and common variants. Nat. Genet. 48:134–143, 2016.

58.

Huebbe

, and Rimbach

Evolution of human apolipoprotein E (APOE) isoforms: gene structure, protein function and interaction with dietary factors. Ageing Res. Rev. 37:146–161, 2017.

59.

Klaver

C.C.

, Kliffen

, van Duijn

C.M.

, Hofman

, Cruts

, Grobbee

D.E.

, van Broeckhoven

, and de Jong

P.T.

Genetic association of apolipoprotein E with age-related macular degeneration. Am. J. Hum. Genet. 63:200–206, 1998.

60.

Schmidt

, Saunders

A.M.

, De La Paz

M.A.

, Postel

E.A.

, Heinis

R.M.

, Agarwal

, Scott

W.K.

, Gilbert

J.R.

, McDowell

J.G.

, Bazyk

, Gass

J.D.

, Haines

J.L.

, and Pericak-Vance

M.A.

Association of the apolipoprotein E gene with age-related macular degeneration: possible effect modification by family history, age, and gender. Mol. Vis. 6:287–293, 2000.

61.

Zareparsi

, Reddick

A.C.

, Branham

K.E.

, Moore

K.B.

, Jessup

, Thoms

, Smith-Wheelock

, Yashar

B.M.

, and Swaroop

Association of apolipoprotein E alleles with susceptibility to age-related macular degeneration in a large cohort from a single center. Invest. Ophthalmol. Vis. Sci. 45:1306–1310, 2004.

62.

McKay

G.J.

, Patterson

C.C.

, Chakravarthy

, Dasari

, Klaver

C.C.

, Vingerling

J.R.

, Ho

, de Jong

P.T.

, Fletcher

A.E.

, Young

I.S.

, Seland

J.H.

, Rahu

, Soubrane

, Tomazzoli

, Topouzis

, Vioque

, Hingorani

A.D.

, Sofat

, Dean

, Sawitzke

, Seddon

J.M.

, Peter

, Webster

A.R.

, Moore

A.T.

, Yates

J.R.

, Cipriani

, Fritsche

L.G.

, Weber

B.H.

, Keilhauer

C.N.

, Lotery

A.J.

, Ennis

, Klein

M.L.

, Francis

P.J.

, Stambolian

, Orlin

, Gorin

M.B.

, Weeks

D.E.

, Kuo

C.L.

, Swaroop

, Othman

, Kanda

, Chen

, Abecasis

G.R.

, Wright

A.F.

, Hayward

, Baird

P.N.

, Guymer

R.H.

, Attia

, Thakkinstian

, and Silvestri

Evidence of association of APOE with age-related macular degeneration: a pooled analysis of 15 studies. Hum. Mutat. 32:1407–1416, 2011.

63.

Chen

, Stambolian

, Edwards

A.O.

, Branham

K.E.

, Othman

, Jakobsdottir

, Tosakulwong

, Pericak-Vance

M.A.

, Campochiaro

P.A.

, Klein

M.L.

, Tan

P.L.

, Conley

Y.P.

, Kanda

, Kopplin

, Li

, Augustaitis

K.J.

, Karoukis

A.J.

, Scott

W.K.

, Agarwal

, Kovach

J.L.

, Schwartz

S.G.

, Postel

E.A.

, Brooks

, Baratz

K.H.

, Brown

W.L.

, Brucker

A.J.

, Orlin

, Brown

, Ho

, Regillo

, Donoso

, Tian

, Kaderli

, Hadley

, Hagstrom

S.A.

, Peachey

N.S.

, Klein

B.E.

, Gotoh

, Yamashiro

, Ferris Iii

, Fagerness

J.A.

, Reynolds

, Farrer

L.A.

, Kim

I.K.

, Miller

J.W.

, Cortón

, Carracedo

, Sanchez-Salorio

, Pugh

E.W.

, Doheny

K.F.

, Brion

, Deangelis

M.M.

, Weeks

D.E.

, Zack

D.J.

, Chew

E.Y.

, Heckenlively

J.R.

, Yoshimura

, Iyengar

S.K.

, Francis

P.J.

, Katsanis

, Seddon

J.M.

, Haines

J.L.

, Gorin

M.B.

, Abecasis

G.R.

, Swaroop

, and Complications of Age-Related Macular Degeneration Prevention Trial Research

Group.

Genetic variants near TIMP3 and high-density lipoprotein-associated loci influence susceptibility to age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 107:7401–7406, 2010.

64.

Neale

B.M.

, Fagerness

, Reynolds

, Sobrin

, Parker

, Raychaudhuri

, Tan

P.L.

, Oh

E.C.

, Merriam

J.E.

, Souied

, Bernstein

P.S.

, Li

, Frederick

J.M.

, Zhang

, Brantley

M.A.

, Lee

A.Y.

, Zack

D.J.

, Campochiaro

, Ripke

, Smith

R.T.

, Barile

G.R.

, Katsanis

, Allikmets

, Daly

M.J.

, and Seddon

J.M.

Genome-wide association study of advanced age-related macular degeneration identifies a role of the hepatic lipase gene (LIPC). Proc. Natl. Acad. Sci. U. S. A. 107:7395–7400, 2010.

65.

Wang

Y.F.

, Han

, Zhang

, Qin

, Wang

M.X.

, and Ma

CETP/LPL/LIPC gene polymorphisms and susceptibility to age-related macular degeneration. Sci. Rep. 5:15711, 2015.

66.

Storti

, Klee

, Todorova

, Steiner

, Othman

, van der Velde-Visser

, Samardzija

, Meneau

, Barben

, Karademir

, Pauzuolyte

, Boye

S.L.

, Blaser

, Ullmer

, Dunaief

J.L.

, Hornemann

, Rohrer

, den Hollander

, von Eckardstein

, Fingerle

, Maugeais

, and Grimm

Impaired ABCA1/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration. Elife. 8: e45100, 2019.

67.

Yvan-Charvet

, Wang

, and Tall

A.R.

Role of HDL, ABCA1, and ABCG1 transporters in cholesterol efflux and immune responses. Arterioscler. Thromb. Vasc. Biol. 30:139–143, 2010.

68.

Ersoy

, Ristau

, Lechanteur

Y.T.

, Hahn

, Hoyng

C.B.

, Kirchhof

, den Hollander

A.I.

, and Fauser

Nutritional risk factors for age-related macular degeneration. Biomed. Res. Int. 2014:413150, 2014.

69.

Howard

K.P.

, Klein

B.E.

, Lee

K.E.

, and Klein

Measures of body shape and adiposity as related to incidence of age-related eye diseases: observations from the Beaver Dam Eye Study. Invest. Ophthalmol. Vis. Sci. 55:2592–2598, 2014.

70.

Ersoy

, Ristau

, Hahn

, Karlstetter

, Langmann

, Droge

, Caramoy

, den Hollander

A.I.

, and Fauser

Genetic and environmental risk factors for age-related macular degeneration in persons 90 years and older. Invest. Ophthalmol. Vis. Sci. 55:1842–1847, 2014.

71.

Smith

, Mitchell

, and Leeder

S.R.

Smoking and age-related maculopathy. The Blue Mountains Eye Study. Arch Ophthalmol. 114:1518–1523, 1996.

72.

Vingerling

J.R.

, Hofman

, Grobbee

D.E.

, and de Jong

P.T.

Age-related macular degeneration and smoking. The Rotterdam Study. Arch. Ophthalmol. 114:1193–1196, 1996.

73.

Armstrong

R.A.

, and Mousavi

Overview of risk factors for age-related macular degeneration (AMD). J. Stem Cells. 10:171–191, 2015.

74.

Clemons

T.E.

, Milton

R.C.

, Klein

, Seddon

J.M.

, and Ferris

F.L.

, 3rd. Risk factors for the incidence of Advanced Age-Related Macular Degeneration in the Age-Related Eye Disease Study (AREDS) AREDS report no. 19. Ophthalmology. 112:533–539, 2005.

75.

Heiba

I.M.

, Elston

R.C.

, Klein

B.E.

, and Klein

Sibling correlations and segregation analysis of age-related maculopathy: the Beaver Dam Eye Study. Genet. Epidemiol. 11:51–67, 1994.

76.

Vingerling

J.R.

, Klaver

C.C.

, Hofman

, and de Jong

P.T.

Epidemiology of age-related maculopathy. Epidemiol. Rev. 17:347–360, 1995.

77.

Fujihara

, Bartels

, Nielsen

L.B.

, and Handa

J.T.

A human apoB100 transgenic mouse expresses human apoB100 in the RPE and develops features of early AMD. Exp. Eye Res. 88:1115–1123, 2009.

78.

Ong

J.M.

, Zorapapel

N.C.

, Rich

K.A.

, Wagstaff

R.E.

, Lambert

R.W.

, Rosenberg

S.E.

, Moghaddas

, Pirouzmanesh

, Aoki

A.M.

, and Kenney

M.C.

Effects of cholesterol and apolipoprotein E on retinal abnormalities in ApoE-deficient mice. Invest. Ophthalmol. Vis. Sci. 42:1891–1900, 2001.

79.

Saadane

, Petrov

, Mast

, El-Darzi

, Dao

, Alnemri

, Song

, Dunaief

J.L.

, and Pikuleva

I.A.

Mechanisms that minimize retinal impact of apolipoprotein E absence. J. Lipid Res. 59:2368–2382, 2018.

80.

Malek

, Johnson

L.V.

, Mace

B.E.

, Saloupis

, Schmechel

D.E.

, Rickman

D.W.

, Toth

C.A.

, Sullivan

P.M.

, and Bowes Rickman

Apolipoprotein E allele-dependent pathogenesis: a model for age-related retinal degeneration. Proc. Natl. Acad. Sci. U. S. A. 102:11900–11905, 2005.

81.

Ding

J.D.

, Johnson

L.V.

, Herrmann

, Farsiu

, Smith

S.G.

, Groelle

, Mace

B.E.

, Sullivan

, Jamison

J.A.

, Kelly

, Harrabi

, Bollini

S.S.

, Dilley

, Kobayashi

, Kuang

, Li

, Pons

, Lin

J.C.

, and Bowes Rickman

Anti-amyloid therapy protects against retinal pigmented epithelium damage and vision loss in a model of age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 108:E279–E287, 2011.

82.

Barathi

V.A.

, Yeo

S.W.

, Guymer

R.H.

, Wong

T.Y.

, and Luu

C.D.

Effects of simvastatin on retinal structure and function of a high-fat atherogenic mouse model of thickened Bruch's membrane. Invest. Ophthalmol. Vis. Sci. 55:460–468, 2014.

83.

Zhao

, Xu

, Jie

, Zuo

, Yu

, Soong

, Sun

, Chen

, and Cai

gammadelta T cells as a major source of IL-17 production during age-dependent RPE degeneration. Invest. Ophthalmol. Vis. Sci. 55:6580–6589, 2014.

84.

Zhang

, Chu

, Mowery

, Konkel

, Galli

, Theos

A.C.

, and Golestaneh

Pgc-1α repression and high-fat diet induce age-related macular degeneration-like phenotypes in mice. Dis. Model. Mech. 11: dmm032698, 2018.

85.

Cho

, Hung

, Willett

W.C.

, Spiegelman

, Rimm

E.B.

, Seddon

J.M.

, Colditz

G.A.

, and Hankinson

S.E.

Prospective study of dietary fat and the risk of age-related macular degeneration. Am. J. Clin. Nutr. 73:209–218, 2001.

86.

Seddon

J.M.

, Rosner

, Sperduto

R.D.

, Yannuzzi

, Haller

J.A.

, Blair

N.P.

, and Willett

Dietary fat and risk for advanced age-related macular degeneration. Arch. Ophthalmol. 119:1191–1199, 2001.

87.

Seddon

J.M.

, Cote

, and Rosner

Progression of age-related macular degeneration: association with dietary fat, transunsaturated fat, nuts, and fish intake. Arch. Ophthalmol. 121:1728–1737, 2003.

88.

Seddon

J.M.

, George

, and Rosner

Cigarette smoking, fish consumption, omega-3 fatty acid intake, and associations with age-related macular degeneration: the US Twin Study of Age-Related Macular Degeneration. Arch. Ophthalmol. 124:995–1001, 2006.

89.

Yiu

, Tieu

, Munevar

, Wong

, Cunefare

, Farsiu

, Garzel

, Roberts

, and Thomasy

S.M.

In vivo multimodal imaging of drusenoid lesions in rhesus macaques. Sci. Rep. 7:15013, 2017.

90.

Francis

P.J.

, Appukuttan

, Simmons

, Landauer

, Stoddard

, Hamon

, Ott

, Ferguson

, Klein

, Stout

J.T.

, and Neuringer

Rhesus monkeys and humans share common susceptibility genes for age-related macular disease. Hum. Mol. Genet. 17:2673–2680, 2008.

91.

Gestri

, Link

B.A.

, and Neuhauss

S.C.

The visual system of zebrafish and its use to model human ocular diseases. Dev. Neurobiol. 72:302–327, 2012.

92.

Chhetri

, Jacobson

, and Gueven

Zebrafish—on the move towards ophthalmological research. Eye (Lond). 28:367–380, 2014.

93.

Kolesnikov

A.V.

, Fan

, Crouch

R.K.

, and Kefalov

V.J.

Age-related deterioration of rod vision in mice. J. Neurosci. 30:11222–11231, 2010.

94.

Williams

G.A.

, and Jacobs

G.H.

Cone-based vision in the aging mouse. Vision Res. 47:2037–2046, 2007.

95.

Mori

, Yamada

, Toyama

, Takahashi

, Akama

, Inoue

, and Nakamura

Developmental and age-related changes to the elastic lamina of Bruch's membrane in mice. Graefes Arch. Clin. Exp. Ophthalmol. 257:289–301, 2019.

96.

Chen

, Rajapakse

, Fraczek

, Luo

, Forrester

J.V.

, and Xu

Retinal pigment epithelial cell multinucleation in the aging eye—a mechanism to repair damage and maintain homoeostasis. Aging Cell. 15:436–445, 2016.

97.

Wolf

N.S.

, Li

, Pendergrass

, Schmeider

, and Turturro

Normal mouse and rat strains as models for age-related cataract and the effect of caloric restriction on its development. Exp. Eye Res. 70:683–692, 2000.

98.

Higuchi

, Macke

E.L.

, Lee

W.H.

, Miller

S.A.

, Xu

J.C.

, Ikeda

, and Ikeda

Genetic basis of age-dependent synaptic abnormalities in the retina. Mamm. Genome. 26:21–32, 2015.

99.

, Chen

, Manivannan

, Lois

, and Forrester

J.V.

Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice. Aging Cell. 7:58–68, 2008.

100.

Choudhary

, Ismail

E.N.

, Yao

P.L.

, Tayyari

, Radu

R.A.

, Nusinowitz

, Boulton

M.E.

, Apte

R.S.

, Ruberti

J.W.

, Handa

J.T.

, Tontonoz

, and Malek

LXRs regulate features of age-related macular degeneration and may be a potential therapeutic target. JCI Insight. 5: e131928, 2020.

101.

Fujihara

, Cano

, and Handa

J.T.

Mice that produce ApoB100 lipoproteins in the RPE do not develop drusen yet are still a valuable experimental system. Invest. Ophthalmol. Vis. Sci. 55:7285–7295, 2014.

102.

, Herrmann

, Bednar

, Saloupis

, Dwyer

M.A.

, Yang

, Qi

, Thomas

R.S.

, Jaffe

G.J.

, Boulton

M.E.

, McDonnell

D.P.

, and Malek

Aryl hydrocarbon receptor deficiency causes dysregulated cellular matrix metabolism and age-related macular degeneration-like pathology. Proc. Natl. Acad. Sci. U. S. A. 110:E4069–E4078, 2013.

103.

Rowan

, Jiang

, Korem

, Szymanski

, Chang

M.L.

, Szelog

, Cassalman

, Dasuri

, McGuire

, Nagai

, Du

X.L.

, Brownlee

, Rabbani

, Thornalley

P.J.

, Baleja

J.D.

, Deik

A.A.

, Pierce

K.A.

, Scott

J.M.

, Clish

C.B.

, Smith

D.E.

, Weinberger

, Avnit-Sagi

, Lotan-Pompan

, Segal

, and Taylor

Involvement of a gut-retina axis in protection against dietary glycemia-induced age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 114:E4472–E4481, 2017.

104.

Weikel

K.A.

, Fitzgerald

, Shang

, Caceres

M.A.

, Bian

, Handa

J.T.

, Stitt

A.W.

, and Taylor

Natural history of age-related retinal lesions that precede AMD in mice fed high or low glycemic index diets. Invest. Ophthalmol. Vis. Sci. 53:622–632, 2012.

105.

Hollyfield

J.G.

, Bonilha

V.L.

, Rayborn

M.E.

, Yang

, Shadrach

K.G.

, Lu

, Ufret

R.L.

, Salomon

R.G.

, and Perez

V.L.

Oxidative damage-induced inflammation initiates age-related macular degeneration. Nat. Med. 14:194–198, 2008.

106.

Hadziahmetovic

, Dentchev

, Song

, Haddad

, He

, Hahn

, Pratico

, Wen

, Harris

Z.L.

, Lambris

J.D.

, Beard

, and Dunaief

J.L.

Ceruloplasmin/hephaestin knockout mice model morphologic and molecular features of AMD. Invest. Ophthalmol. Vis. Sci. 49:2728–2736, 2008.

107.

Hahn

, Qian

, Dentchev

, Chen

, Beard

, Harris

Z.L.

, and Dunaief

J.L.

Disruption of ceruloplasmin and hephaestin in mice causes retinal iron overload and retinal degeneration with features of age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 101:13850–13855, 2004.

108.

Coffey

P.J.

, Gias

, McDermott

C.J.

, Lundh

, Pickering

M.C.

, Sethi

, Bird

, Fitzke

F.W.

, Maass

, Chen

L.L.

, Holder

G.E.

, Luthert

P.J.

, Salt

T.E.

, Moss

S.E.

, and Greenwood

Complement factor H deficiency in aged mice causes retinal abnormalities and visual dysfunction. Proc. Natl. Acad. Sci. U. S. A. 104:16651–16656, 2007.

109.

Ding

J.D.

, Kelly

, Landowski

, Toomey

C.B.

, Groelle

, Miller

, Smith

S.G.

, Klingeborn

, Singhapricha

, Jiang

, Frank

M.M.

, and Bowes Rickman

Expression of human complement factor h prevents age-related macular degeneration-like retina damage and kidney abnormalities in aged cfh knockout mice. Am. J. Pathol. 185:29–42, 2015.

110.

Ufret-Vincenty

R.L.

, Aredo

, Liu

, McMahon

, Chen

P.W.

, Sun

, Niederkorn

J.Y.

, and Kedzierski

Transgenic mice expressing variants of complement factor H develop AMD-like retinal findings. Invest. Ophthalmol. Vis. Sci. 51:5878–5887, 2010.

111.

Lennikov

, Saddala

M.S.

, Mukwaya

, Tang

, and Huang

Autoimmune-mediated retinopathy in CXCR5-deficient mice as the result of age-related macular degeneration associated proteins accumulation. Front. Immunol. 10:1903, 2019.

112.

Lennikov

, Mukwaya

, Saddala

M.S.

, and Huang

Deficiency of C-X-C chemokine receptor type 5 (CXCR5) gene causes dysfunction of retinal pigment epithelium cells. Lab. Invest. 101:228–244, 2021.

113.

Huang

, Liu

, Wang

, and Li

Age-related macular degeneration phenotypes are associated with increased tumor necrosis-alpha and subretinal immune cells in aged Cxcr5 knockout mice. PLoS One. 12:e0173716, 2017.

114.

Notomi

, Ishihara

, Efstathiou

N.E.

, Lee

J.J.

, Hisatomi

, Tachibana

, Konstantinou

E.K.

, Ueta

, Murakami

, Maidana

D.E.

, Ikeda

, Kume

, Terasaki

, Sonoda

, Blanz

, Young

, Sakamoto

, Sonoda

K.H.

, Saftig

, Ishibashi

, Miller

J.W.

, Kroemer

, and Vavvas

D.G.

Genetic LAMP2 deficiency accelerates the age-associated formation of basal laminar deposits in the retina. Proc. Natl. Acad. Sci. U. S. A. 116:23724–23734, 2019.

115.

Hashizume

, Hirasawa

, Imamura

, Noda

, Shimizu

, Shinoda

, Kurihara

, Noda

, Ozawa

, Ishida

, Miyake

, Shirasawa

, and Tsubota

Retinal dysfunction and progressive retinal cell death in SOD1-deficient mice. Am. J. Pathol. 172:1325–1331, 2008.

116.

Imamura

, Noda

, Hashizume

, Shinoda

, Yamaguchi

, Uchiyama

, Shimizu

, Mizushima

, Shirasawa

, and Tsubota

Drusen, choroidal neovascularization, and retinal pigment epithelium dysfunction in SOD1-deficient mice: a model of age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 103:11282–11287, 2006.

117.

Justilien

, Pang

J.J.

, Renganathan

, Zhan

, Crabb

J.W.

, Kim

S.R.

, Sparrow

J.R.

, Hauswirth

W.W.

, and Lewin

A.S.

SOD2 knockdown mouse model of early AMD. Invest. Ophthalmol. Vis. Sci. 48:4407–4420, 2007.

118.

Mao

, Seo

S.J.

, Biswal

M.R.

, Li

, Conners

, Nandyala

, Jones

, Le

Y.Z.

, and Lewin

A.S.

Mitochondrial oxidative stress in the retinal pigment epithelium leads to localized retinal degeneration. Invest. Ophthalmol. Vis. Sci. 55:4613–4627, 2014.

119.

Nakayama

, Iejima

, Akahori

, Kamei

, Goto

, and Iwata

Overexpression of HtrA1 and exposure to mainstream cigarette smoke leads to choroidal neovascularization and subretinal deposits in aged mice. Invest. Ophthalmol. Vis. Sci. 55:6514–6523, 2014.

120.

Wright

C.B.

, Uehara

, Kim

, Yasuma

, Hirahara

, Makin

R.D.

, Apicella

, Pereira

, Nagasaka

, Narendran

, Fukuda

, Albuquerque

, Fowler

B.J.

, Bastos-Carvalho

, Georgel

, Hatada

, Chang

, Kerur

, Ambati

B.K.

, Ambati

, and Gelfand

B.D.

Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc. Natl. Acad. Sci. U. S. A. 117:2579–2587, 2020.

121.

Landowski

, Grindel

, Shahi

P.K.

, Johnson

, Western

, Race

, Shi

, Benson

, Gao

, Santoirre

, Lee

W.H.

, Ikeda

, Pattnaik

B.R.

, and Ikeda

Modulation of Tmem135 leads to retinal pigmented epithelium pathologies in mice. Invest. Ophthalmol. Vis. Sci. 61:16, 2020.

122.

Cheng

S.Y.

, Cipi

, Ma

, Hafler

B.P.

, Kanadia

R.N.

, Brush

R.S.

, Agbaga

M.P.

, and Punzo

Altered photoreceptor metabolism in mouse causes late stage age-related macular degeneration-like pathologies. Proc. Natl. Acad. Sci. U. S. A. 117:13094–13104, 2020.

123.

Choudhary

, Ding

J.D.

, Qi

, Boulton

M.E.

, Yao

P.L.

, Peters

J.M.

, and Malek

PPARβ/δ selectively regulates phenotypic features of age-related macular degeneration. Aging (Albany NY). 8:1952–1978, 2016.

124.

Lyzogubov

V.V.

, Bora

P.S.

, Wu

, Horn

L.E.

, de Roque

, Rudolf

X.V.

, Atkinson

J.P.

, and Bora

N.S.

The complement regulatory protein CD46 deficient mouse spontaneously develops dry-type age-related macular degeneration-like phenotype. Am. J. Pathol. 186:2088–2104, 2016.

125.

Franco

L.M.

, Zulliger

, Wolf-Schnurrbusch

U.E.

, Katagiri

, Kaplan

H.J.

, Wolf

, and Enzmann

Decreased visual function after patchy loss of retinal pigment epithelium induced by low-dose sodium iodate. Invest. Ophthalmol. Vis. Sci. 50:4004–4010, 2009.

126.

Enzmann

, Row

B.W.

, Yamauchi

, Kheirandish

, Gozal

, Kaplan

H.J.

, and McCall

M.A.

Behavioral and anatomical abnormalities in a sodium iodate-induced model of retinal pigment epithelium degeneration. Exp. Eye Res. 82:441–448, 2006.

127.

Lambert

, Lecomte

, Hansen

, Blacher

, Gonzalez

M.L.

, Struman

, Sounni

N.E.

, Rozet

, de Tullio

, Foidart

J.M.

, Rakic

J.M.

, and Noel

Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice. Nat. Protoc. 8:2197–2211, 2013.

128.

Jones

, Kumar

, Zhang

, Tong

, Yang

J.H.

, Watt

, Anderson

, Amrita

Fillerup

, H., McCloskey

, Luo

, Yang

, Ambati

, Marc

, Oka

, Zhang

, and Fu

Increased expression of multifunctional serine protease, HTRA1, in retinal pigment epithelium induces polypoidal choroidal vasculopathy in mice. Proc. Natl. Acad. Sci. U. S. A. 108:14578–14583, 2011.

129.

Vierkotten

, Muether

P.S.

, and Fauser

Overexpression of HTRA1 leads to ultrastructural changes in the elastic layer of Bruch's membrane via cleavage of extracellular matrix components. PLoS One. 6:e22959, 2011.

130.

Williams

B.L.

, Seager

N.A.

, Gardiner

J.D.

, Pappas

C.M.

, Cronin

M.C.

, Amat di San Filippo

, Anstadt

R.A.

, Liu

, Toso

M.A.

, Nichols

, Parnell

T.J.

, Eve

J.R.

, Bartel

P.L.

, Zouache

M.A.

, Richards

B.T.

, and Hageman

G.S.

Chromosome 10q26-driven age-related macular degeneration is associated with reduced levels of HTRA1 in human retinal pigment epithelium. Proc. Natl. Acad. Sci. U. S. A. 118:e2103617118, 2021.

131.

B.X.

, Fan

, Boyer

N.P.

, Jenkins

R.W.

, Koutalos

, Hannun

Y.A.

, and Crosson

C.E.

Lack of acid sphingomyelinase induces age-related retinal degeneration. PLoS One. 10:e0133032, 2015.

132.

Fujihara

, Nagai

, Sussan

T.E.

, Biswal

, and Handa

J.T.

Chronic cigarette smoke causes oxidative damage and apoptosis to retinal pigmented epithelial cells in mice. PLoS One. 3:e3119, 2008.

133.

Doyle

S.L.

, Campbell

, Ozaki

, Salomon

R.G.

, Mori

, Kenna

P.F.

, Farrar

G.J.

, Kiang

A.S.

, Humphries

M.M.

, Lavelle

E.C.

, O'Neill

L.A.

, Hollyfield

J.G.

, and Humphries

NLRP3 has a protective role in age-related macular degeneration through the induction of IL-18 by drusen components. Nat. Med. 18:791–798, 2012.

134.

Cruz-Guilloty

, Saeed

A.M.

, Echegaray

J.J.

, Duffort

, Ballmick

, Tan

, Betancourt

, Viteri

, Ramkhellawan

G.C.

, Ewald

, Feuer

, Huang

, Wen

, Hong

, Wang

, Laird

J.M.

, Sene

, Apte

R.S.

, Salomon

R.G.

, Hollyfield

J.G.

, and Perez

V.L.

Infiltration of proinflammatory m1 macrophages into the outer retina precedes damage in a mouse model of age-related macular degeneration. Int. J. Inflam. 2013:503725, 2013.

135.

Schnabolk

, Obert

, Banda

N.K.

, and Rohrer

Systemic inflammation by collagen-induced arthritis affects the progression of age-related macular degeneration differently in two mouse models of the disease. Invest. Ophthalmol. Vis. Sci. 61:11, 2020.

136.

Hadziahmetovic

, Song

, Wolkow

, Iacovelli

, Grieco

, Lee

, Lyubarsky

, Pratico

, Connelly

, Spino

, Harris

Z.L.

, and Dunaief

J.L.

The oral iron chelator deferiprone protects against iron overload-induced retinal degeneration. Invest. Ophthalmol. Vis. Sci. 52:959–968, 2011.

137.

Lundh von Leithner

, Kam

J.H.

, Bainbridge

, Catchpole

, Gough

, Coffey

, and Jeffery

Complement factor h is critical in the maintenance of retinal perfusion. Am. J. Pathol. 175:412–421, 2009.

138.

Toomey

C.B.

, Landowski

, Klingeborn

, Kelly

, Deans

, Dong

, Harrabi

, Van Blarcom

, Yeung

Y.A.

, Grishanin

, Lin

J.C.

, Saban

D.R.

, and Bowes Rickman

Effect of anti-C5a therapy in a murine model of early/intermediate dry age-related macular degeneration. Invest. Ophthalmol. Vis. Sci. 59:662–673, 2018.

139.

Lesher

A.M.

, Zhou

, Kimura

, Sato

, Gullipalli

, Herbert

A.P.

, Barlow

P.N.

, Eberhardt

H.U.

, Skerka

, Zipfel

P.F.

, Hamano

, Miwa

, Tung

K.S.

, and Song

W.C.

Combination of factor H mutation and properdin deficiency causes severe C3 glomerulonephritis. J. Am. Soc. Nephrol. 24:53–65, 2013.

140.

Song

, Mohammed

, Bhuyan

, Miwa

, Williams

A.L.

, Gullipalli

, Sato

, Song

, Dunaief

J.L.

, and Song

W.C.

Retinal basal laminar deposits in complement fH/fP mouse model of dense deposit disease. Invest. Ophthalmol. Vis. Sci. 59:3405–3415, 2018.

141.

Huang

, and Lennikov

CXCR5/NRF2 double knockout mice develop retinal degeneration phenotype at early adult age. Exp. Eye Res. 196:108061, 2020.

142.

Dhingra

, Bell

B.A.

, Peachey

N.S.

, Daniele

L.L.

, Reyes-Reveles

, Sharp

R.C.

, Jun

, Bazan

N.G.

, Sparrow

J.R.

, Kim

H.J.

, Philp

N.J.

, and Boesze-Battaglia

Microtubule-associated protein 1 light chain 3B, (LC3B) is necessary to maintain lipid-mediated homeostasis in the retinal pigment epithelium. Front. Cell Neurosci. 12:351, 2018.

143.

Felszeghy

, Viiri

, Paterno

J.J.

, Hyttinen

J.M.T.

, Koskela

, Chen

, Leinonen

, Tanila

, Kivinen

, Koistinen

, Toropainen

, Amadio

, Smedowski

, Reinisalo

, Winiarczyk

, Mackiewicz

, Mutikainen

, Ruotsalainen

A.K.

, Kettunen

, Jokivarsi

, Sinha

, Kinnunen

, Petrovski

, Blasiak

, Bjørkøy

, Koskelainen

, Skottman

, Urtti

, Salminen

, Kannan

, Ferrington

D.A.

, Xu

, Levonen

A.L.

, Tavi

, Kauppinen

, and Kaarniranta

Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration. Redox Biol. 20:1–12, 2019.

144.

Yao

, Jia

, Khan

, Lin

, Mitter

S.K.

, Boulton

M.E.

, Dunaief

J.L.

, Klionsky

D.J.

, Guan

J.L.

, Thompson

D.A.

, and Zacks

D.N.

Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium. Autophagy. 11:939–953, 2015.

145.

Dong

, Shen

, Krause

, Akiyama

, Hackett

S.F.

, Lai

, and Campochiaro

P.A.

Superoxide dismutase 1 protects retinal cells from oxidative damage. J. Cell. Physiol. 208:516–526, 2006.

146.

, Pay

S.L.

, Yan

, Thomas

Jr. , Lewin

A.S.

, Chang

L.J.

, Grant

M.B.

, and Boulton

M.E.

Systemic injection of RPE65-programmed bone marrow-derived cells prevents progression of chronic retinal degeneration. Mol. Ther. 25:917–927, 2017.

147.

Choudhary

, Kazmin

, Hu

, Thomas

R.S.

, McDonnell

D.P.

, and Malek

Aryl hydrocarbon receptor knock-out exacerbates choroidal neovascularization via multiple pathogenic pathways. J. Pathol. 235:101–112, 2015.

148.

Combadiere

, Feumi

, Raoul

, Keller

, Rodero

, Pezard

, Lavalette

, Houssier

, Jonet

, Picard

, Debre

, Sirinyan

, Deterre

, Ferroukhi

, Cohen

S.Y.

, Chauvaud

, Jeanny

J.C.

, Chemtob

, Behar-Cohen

, and Sennlaub

CX3CR1-dependent subretinal microglia cell accumulation is associated with cardinal features of age-related macular degeneration. J. Clin. Invest. 117:2920–2928, 2007.

149.

Krzystolik

M.G.

, Afshari

M.A.

, Adamis

A.P.

, Gaudreault

, Gragoudas

E.S.

, Michaud

N.A.

, Li

, Connolly

, O'Neill

C.A.

, and Miller

J.W.

Prevention of experimental choroidal neovascularization with intravitreal anti-vascular endothelial growth factor antibody fragment. Arch. Ophthalmol. 120:338–346, 2002.

150.

Lyzogubov

V.V.

, Tytarenko

R.G.

, Liu

, Bora

N.S.

, and Bora

P.S.

Polyethylene glycol (PEG)-induced mouse model of choroidal neovascularization. J. Biol. Chem. 286:16229–16237, 2011.

151.

, Huang

, Kingsley

, Zhou

, Li

, Parke

D.W.

, and Cao

Biochemical alterations in the retinas of very low-density lipoprotein receptor knockout mice: an animal model of retinal angiomatous proliferation. Arch. Ophthalmol. 125:795–803, 2007.

152.

Tarallo

, Hirano

, Gelfand

B.D.

, Dridi

, Kerur

, Kim

, Cho

W.G.

, Kaneko

, Fowler

B.J.

, Bogdanovich

, Albuquerque

R.J.

, Hauswirth

W.W.

, Chiodo

V.A.

, Kugel

J.F.

, Goodrich

J.A.

, Ponicsan

S.L.

, Chaudhuri

, Murphy

M.P.

, Dunaief

J.L.

, Ambati

B.K.

, Ogura

, Yoo

J.W.

, Lee

D.K.

, Provost

, Hinton

D.R.

, Nunez

, Baffi

J.Z.

, Kleinman

M.E.

, and Ambati

DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88. Cell. 149:847–859, 2012.

153.

Kaneko

, Dridi

, Tarallo

, Gelfand

B.D.

, Fowler

B.J.

, Cho

W.G.

, Kleinman

M.E.

, Ponicsan

S.L.

, Hauswirth

W.W.

, Chiodo

V.A.

, Kariko

, Yoo

J.W.

, Lee

D.K.

, Hadziahmetovic

, Song

, Misra

, Chaudhuri

, Buaas

F.W.

, Braun

R.E.

, Hinton

D.R.

, Zhang

, Grossniklaus

H.E.

, Provis

J.M.

, Madigan

M.C.

, Milam

A.H.

, Justice

N.L.

, Albuquerque

R.J.

, Blandford

A.D.

, Bogdanovich

, Hirano

, Witta

, Fuchs

, Littman

D.R.

, Ambati

B.K.

, Rudin

C.M.

, Chong

M.M.

, Provost

, Kugel

J.F.

, Goodrich

J.A.

, Dunaief

J.L.

, Baffi

J.Z.

, and Ambati

DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. Nature. 471:325–330, 2011.

154.

Longbottom

, Fruttiger

, Douglas

R.H.

, Martinez-Barbera

J.P.

, Greenwood

, and Moss

S.E.

Genetic ablation of retinal pigment epithelial cells reveals the adaptive response of the epithelium and impact on photoreceptors. Proc. Natl. Acad. Sci. U. S. A. 106:18728–18733, 2009.

155.

Chowers

, Cohen

, Marks-Ohana

, Stika

, Eijzenberg

, Banin

, and Obolensky

Course of sodium iodate-induced retinal degeneration in albino and pigmented mice. Invest. Ophthalmol. Vis. Sci. 58:2239–2249, 2017.

156.

Ibbett

, Goverdhan

S.V.

, Pipi

, Chouhan

J.K.

, Keeling

, Angus

E.M.

, Scott

J.A.

, Gatherer

, Page

, Teeling

J.L.

, Lotery

A.J.

, and Arjuna Ratnayaka

A lasered mouse model of retinal degeneration displays progressive outer retinal pathology providing insights into early geographic atrophy. Sci. Rep. 9:7475, 2019.

157.

O'Koren

E.G.

, Mathew

, and Saban

D.R.

Fate mapping reveals that microglia and recruited monocyte-derived macrophages are definitively distinguishable by phenotype in the retina. Sci. Rep. 6:20636, 2016.

158.

Cachafeiro

, Bemelmans

A.P.

, Samardzija

, Afanasieva

, Pournaras

J.A.

, Grimm

, Kostic

, Philippe

, Wenzel

, and Arsenijevic

Hyperactivation of retina by light in mice leads to photoreceptor cell death mediated by VEGF and retinal pigment epithelium permeability. Cell Death Dis. 4:e781, 2013.

159.

Chen

, Wu

, Dentchev

, Zeng

, Wang

, Tsui

, Tobias

J.W.

, Bennett

, Baldwin

, and Dunaief

J.L.

Light damage induced changes in mouse retinal gene expression. Exp. Eye Res. 79:239–247, 2004.

160.

Grimm

, and Reme

C.E.

Light damage as a model of retinal degeneration. Methods Mol. Biol. 935:87–97, 2013.

161.

Cingolani

, Rogers

, Lu

, Kachi

, Shen

, and Campochiaro

P.A.

Retinal degeneration from oxidative damage. Free Radic. Biol. Med. 40:660–669, 2006.

162.

Espinosa-Heidmann

D.G.

, Suner

I.J.

, Catanuto

, Hernandez

E.P.

, Marin-Castano

M.E.

, and Cousins

S.W.

Cigarette smoke-related oxidants and the development of sub-RPE deposits in an experimental animal model of dry AMD. Invest. Ophthalmol. Vis. Sci. 47:729–737, 2006.

163.

Montalbán-Soler

, Alarcón-Martínez

, Jiménez-López

, Salinas-Navarro

, Galindo-Romero

, Bezerra de Sá

, García-Ayuso

, Avilés-Trigueros

, Vidal-Sanz

, Agudo-Barriuso

, and Villegas-Pérez

M.P.

Retinal compensatory changes after light damage in albino mice. Mol. Vis. 18:675–693, 2012.

164.

Radu

R.A.

, Hu

, Yuan

, Welch

D.L.

, Makshanoff

, Lloyd

, McMullen

, Travis

G.H.

, and Bok

Complement system dysregulation and inflammation in the retinal pigment epithelium of a mouse model for Stargardt macular degeneration. J. Biol. Chem. 286:18593–18601, 2011.

165.

Chen

, Palczewska

, Mustafi

, Golczak

, Dong

, Sawada

, Maeda

, and Palczewski

Systems pharmacology identifies drug targets for Stargardt disease-associated retinal degeneration. J. Clin. Invest. 123:5119–5134, 2013.

166.

Weng

, Mata

N.L.

, Azarian

S.M.

, Tzekov

R.T.

, Birch

D.G.

, and Travis

G.H.

Insights into the function of Rim protein in photoreceptors and etiology of Stargardt's disease from the phenotype in abcr knockout mice. Cell. 98:13–23, 1999.

167.

Charbel Issa

, Barnard

A.R.

, Singh

M.S.

, Carter

, Jiang

, Radu

R.A.

, Schraermeyer

, and MacLaren

R.E.

Fundus autofluorescence in the Abca4(−/−) mouse model of Stargardt disease—correlation with accumulation of A2E, retinal function, and histology. Invest. Ophthalmol. Vis. Sci. 54:5602–5612, 2013.

168.

Chavali

V.R.

, Khan

N.W.

, Cukras

C.A.

, Bartsch

D.U.

, Jablonski

M.M.

, and Ayyagari

A CTRP5 gene S163R mutation knock-in mouse model for late-onset retinal degeneration. Hum. Mol. Genet. 20:2000–2014, 2011.

169.

Dinculescu

, Min

S.H.

, Dyka

F.M.

, Deng

W.T.

, Stupay

R.M.

, Chiodo

, Smith

W.C.

, and Hauswirth

W.W.

Pathological effects of mutant C1QTNF5 (S163R) expression in murine retinal pigment epithelium. Invest. Ophthalmol. Vis. Sci. 56:6971–6980, 2015.

170.

Garland

D.L.

, Fernandez-Godino

, Kaur

, Speicher

K.D.

, Harnly

J.M.

, Lambris

J.D.

, Speicher

D.W.

, and Pierce

E.A.

Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum. Mol. Genet. 23:52–68, 2014.

171.

Fernandez-Godino

, Garland

D.L.

, and Pierce

E.A.

A local complement response by RPE causes early-stage macular degeneration. Hum. Mol. Genet. 24:5555–5569, 2015.

172.

, Garland

, Yang

, Shukla

, Rajendran

, Pearson

, Stone

E.M.

, Zhang

, and Pierce

E.A.

The R345W mutation in EFEMP1 is pathogenic and causes AMD-like deposits in mice. Hum. Mol. Genet. 16:2411–2422, 2007.

173.

Marmorstein

L.Y.

, McLaughlin

P.J.

, Peachey

N.S.

, Sasaki

, and Marmorstein

A.D.

Formation and progression of sub-retinal pigment epithelium deposits in Efemp1 mutation knock-in mice: a model for the early pathogenic course of macular degeneration. Hum. Mol. Genet. 16:2423–2432, 2007.

174.

Mandal

N.A.

, Tran

J.T.

, Zheng

, Wilkerson

J.L.

, Brush

R.S.

, McRae

, Agbaga

M.P.

, Zhang

, Petrukhin

, Ayyagari

, and Anderson

R.E.

In vivo effect of mutant ELOVL4 on the expression and function of wild-type ELOVL4. Invest. Ophthalmol. Vis. Sci. 55:2705–2713, 2014.

175.

Karan

, Lillo

, Yang

, Cameron

D.J.

, Locke

K.G.

, Zhao

, Thirumalaichary

, Li

, Birch

D.G.

, Vollmer-Snarr

H.R.

, Williams

D.S.

, and Zhang

Lipofuscin accumulation, abnormal electrophysiology, and photoreceptor degeneration in mutant ELOVL4 transgenic mice: a model for macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 102:4164–4169, 2005.

176.

Vasireddy

, Jablonski

M.M.

, Mandal

M.N.

, Raz-Prag

, Wang

X.F.

, Nizol

, Iannaccone

, Musch

D.C.

, Bush

R.A.

, Salem

Jr. , Sieving

P.A.

, and Ayyagari

Elovl4 5-bp-deletion knock-in mice develop progressive photoreceptor degeneration. Invest. Ophthalmol. Vis. Sci. 47:4558–4568, 2006.

177.

J.H.

, Dai

, Luthert

, Chaurasia

, Hollyfield

, Weber

B.H.

, Stohr

, and Anand-Apte

S156C mutation in tissue inhibitor of metalloproteinases-3 induces increased angiogenesis. J. Biol. Chem. 284:19927–19936, 2009.

178.

Weber

B.H.

, Lin

, White

, Kohler

, Soboleva

, Herterich

, Seeliger

M.W.

, Jaissle

G.B.

, Grimm

, Reme

, Wenzel

, Asan

, and Schrewe

A mouse model for Sorsby fundus dystrophy. Invest. Ophthalmol. Vis. Sci. 43:2732–2740, 2002.

179.

Grossniklaus

H.E.

, Kang

S.J.

, and Berglin

Animal models of choroidal and retinal neovascularization. Prog. Retin. Eye Res. 29:500–519, 2010.

180.

Poor

S.H.

, Qiu

, Fassbender

E.S.

, Shen

, Woolfenden

, Delpero

, Kim

, Buchanan

, Gebuhr

T.C.

, Hanks

S.M.

, Meredith

E.L.

, Jaffee

B.D.

, and Dryja

T.P.

Reliability of the mouse model of choroidal neovascularization induced by laser photocoagulation. Invest. Ophthalmol. Vis. Sci. 55:6525–6534, 2014.

181.

Yanai

, Mulki

, Hasegawa

, Takeuchi

, Sweigard

, Suzuki

, Gaissert

, Vavvas

D.G.

, Sonoda

K.H.

, Rothe

, Schunck

W.H.

, Miller

J.W.

, and Connor

K.M.

Cytochrome P450-generated metabolites derived from ω-3 fatty acids attenuate neovascularization. Proc. Natl. Acad. Sci. U. S. A. 111:9603–9608, 2014.

182.

Yanai

, Chen

, Uchi

S.H.

, Nanri

, Connor

K.M.

, and Kimura

Attenuation of choroidal neovascularization by dietary intake of ω-3 long-chain polyunsaturated fatty acids and lutein in mice. PLoS One. 13:e0196037, 2018.

183.

, Liegl

, Wang

, Gong

, Liu

C.H.

, Sun

, Cakir

, Burnim

S.B.

, Meng

S.S.

, Löfqvist

, SanGiovanni

J.P.

, Hellström

, and Smith

L.E.H.

Adiponectin mediates dietary omega-3 long-chain polyunsaturated fatty acid protection against choroidal neovascularization in mice. Invest. Ophthalmol. Vis. Sci. 58:3862–3870, 2017.

184.

Koto

, Nagai

, Mochimaru

, Kurihara

, Izumi-Nagai

, Satofuka

, Shinoda

, Noda

, Ozawa

, Inoue

, Tsubota

, Oike

, and Ishida

Eicosapentaenoic acid is anti-inflammatory in preventing choroidal neovascularization in mice. Invest. Ophthalmol. Vis. Sci. 48:4328–4334, 2007.

185.

Zhu

, Parker

, Enemchukwu

, Shen

, Zhang

, Yan

, Handa

J.T.

, Fang

, and Fu

Combination of apolipoprotein-A-I/apolipoprotein-A-I binding protein and anti-VEGF treatment overcomes anti-VEGF resistance in choroidal neovascularization in mice. Commun. Biol. 3:386, 2020.

186.

Terao

, Honjo

, and Aihara

Apolipoprotein M inhibits angiogenic and inflammatory response by sphingosine 1-phosphate on retinal pigment epithelium cells. Int. J. Mol. Sci. 19: 112, 2017.

187.

Suda

, Murakami

, Gotoh

, Fukuda

, Hashida

, Tsujikawa

, and Yoshimura

High-density lipoprotein mutant eye drops for the treatment of posterior eye diseases. J. Control. Release. 266:301–309, 2017.

188.

Ivanescu

A.A.

, Fernández-Robredo

, Heras-Mulero

, Sádaba-Echarri

L.M.

, García-García

, Fernández-García

, Moreno-Orduna

, Redondo-Exposito

, Recalde

, and García-Layana

Modifying choroidal neovascularization development with a nutritional supplement in mice. Nutrients. 7:5423–5442, 2015.

189.

Yamada

, Sakurai

, Itaya

, Yamasaki

, and Ogura

Inhibition of laser-induced choroidal neovascularization by atorvastatin by downregulation of monocyte chemotactic protein-1 synthesis in mice. Invest. Ophthalmol. Vis. Sci. 48:1839–1843, 2007.

190.

Zambarakji

H.J.

, Nakazawa

, Connolly

, Lane

A.M.

, Mallemadugula

, Kaplan

, Michaud

, Hafezi-Moghadam

, Gragoudas

E.S.

, and Miller

J.W.

Dose-dependent effect of pitavastatin on VEGF and angiogenesis in a mouse model of choroidal neovascularization. Invest. Ophthalmol. Vis. Sci. 47:2623–2631, 2006.

191.

Gong

, Fu

, Edin

M.L.

, Liu

C.H.

, Wang

, Shao

, Fredrick

T.W.

, Saba

N.J.

, Morss

P.C.

, Burnim

S.B.

, Meng

S.S.

, Lih

F.B.

, Lee

K.S.

, Moran

E.P.

, SanGiovanni

J.P.

, Hellström

, Hammock

B.D.

, Zeldin

D.C.

, and Smith

L.E.

Cytochrome P450 oxidase 2C inhibition adds to ω-3 long-chain polyunsaturated fatty acids protection against retinal and choroidal neovascularization. Arterioscler. Thromb. Vasc. Biol. 36:1919–1927, 2016.

192.

Hayward

, Shu

, Cideciyan

A.V.

, Lennon

, Barran

, Zareparsi

, Sawyer

, Hendry

, Dhillon

, Milam

A.H.

, Luthert

P.J.

, Swaroop

, Hastie

N.D.

, Jacobson

S.G.

, and Wright

A.F.

Mutation in a short-chain collagen gene, CTRP5, results in extracellular deposit formation in late-onset retinal degeneration: a genetic model for age-related macular degeneration. Hum. Mol. Genet. 12:2657–2667, 2003.

193.

Stone

E.M.

, Lotery

A.J.

, Munier

F.L.

, Heon

, Piguet

, Guymer

R.H.

, Vandenburgh

, Cousin

, Nishimura

, Swiderski

R.E.

, Silvestri

, Mackey

D.A.

, Hageman

G.S.

, Bird

A.C.

, Sheffield

V.C.

, and Schorderet

D.F.

A single EFEMP1 mutation associated with both Malattia Leventinese and Doyne honeycomb retinal dystrophy. Nat. Genet. 22:199–202, 1999.

194.

Weber

, Vogt

, Pruett

, Stohr

, and Felbor

Mutations in the tissue inhibitor of metalloproteinases-3 (TIMP3) in patients with Sorsby's fundus dystrophy. Nat. Genet. 8:352–356, 1994.

195.

Zhang

, Kniazeva

, Han

, Li

, Yu

, Yang

, Li

, Metzker

M.L.

, Allikmets

, Zack

D.J.

, Kakuk

L.E.

, Lagali

P.S.

, Wong

P.W.

, MacDonald

I.M.

, Sieving

P.A.

, Figueroa

D.J.

, Austin

C.P.

, Gould

R.J.

, Ayyagari

, and Petrukhin

A 5-bp deletion in ELOVL4 is associated with two related forms of autosomal dominant macular dystrophy. Nat. Genet. 27:89–93, 2001.

196.

Allikmets

, Shroyer

N.F.

, Singh

, Seddon

J.M.

, Lewis

R.A.

, Bernstein

P.S.

, Peiffer

, Zabriskie

N.A.

, Li

, Hutchinson

, Dean

, Lupski

J.R.

, and Leppert

Mutation of the Stargardt disease gene (ABCR) in age-related macular degeneration. Science. 277:1805–1807, 1997.

197.

Maeda

, Golczak

, Maeda

, and Palczewski

Limited roles of Rdh8, Rdh12, and Abca4 in all-trans-retinal clearance in mouse retina. Invest. Ophthalmol. Vis. Sci. 50:5435–5443, 2009.

198.

Maeda

, Maeda

, Golczak

, Chou

, Desai

, Hoppel

C.L.

, Matsuyama

, and Palczewski

Involvement of all-trans-retinal in acute light-induced retinopathy of mice. J. Biol. Chem. 284:15173–15183, 2009.

199.

Mattapallil

M.J.

, Wawrousek

E.F.

, Chan

C.C.

, Zhao

, Roychoudhury

, Ferguson

T.A.

, and Caspi

R.R.

The Rd8 mutation of the Crb1 gene is present in vendor lines of C57BL/6N mice and embryonic stem cells, and confounds ocular induced mutant phenotypes. Invest. Ophthalmol. Vis. Sci. 53:2921–2927, 2012.

200.

Bowes

, Li

, Danciger

, Baxter

L.C.

, Applebury

M.L.

, and Farber

D.B.

Retinal degeneration in the rd mouse is caused by a defect in the b subunit of rod cGMP-phosphodiesterase. Nature. 347:677–680, 1990.

201.

Bowes

, Li

, Frankel

W.N.

, Danciger

, Coffin

J.M.

, Applebury

M.L.

, and Farber

D.B.

Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase. Proc. Natl. Acad. Sci. U. S. A. 90:2955–2959, 1993.

202.

Neuner

S.M.

, Heuer

S.E.

, Huentelman

M.J.

, O'Connell

K.M.S.

, and Kaczorowski

C.C.

Harnessing genetic complexity to enhance translatability of Alzheimer's disease mouse models: a path toward precision medicine. Neuron. 101:399–411.e395, 2019.

203.

Sundberg

J.P.

, and Schofield

P.N.

Living inside the box: environmental effects on mouse models of human disease. Dis. Model. Mech. 11: dmm035360, 2018.

204.

Hoshino

, Ratnapriya

, Brooks

M.J.

, Chaitankar

, Wilken

M.S.

, Zhang

, Starostik

M.R.

, Gieser

, La Torre

, Nishio

, Bates

, Walton

, Bermingham-McDonogh

, Glass

I.A.

, Wong

R.O.L.

, Swaroop

, and Reh

T.A.

Molecular anatomy of the developing human retina. Dev. Cell. 43:763–779.e764, 2017.

205.

Volland

, Esteve-Rudd

, Hoo

, Yee

, and Williams

D.S.

A comparison of some organizational characteristics of the mouse central retina and the human macula. PLoS One. 10:e0125631, 2015.

206.

Curcio

C.A.

, Medeiros

N.E.

, and Millican

C.L.

Photoreceptor loss in age-related macular degeneration. Invest. Ophthalmol. Vis. Sci. 37:1236–1249, 1996.

207.

Huberman

A.D.

, and Niell

C.M.

What can mice tell us about how vision works?. Trends Neurosci. 34:464–473, 2011.

208.

Ingram

N.T.

, Fain

G.L.

, and Sampath

A.P.

Elevated energy requirement of cone photoreceptors. Proc. Natl. Acad. Sci. U. S. A. 117:19599–19603, 2020.

209.

Mustafi

, Kevany

B.M.

, Bai

, Golczak

, Adams

M.D.

, Wynshaw-Boris

, and Palczewski

Transcriptome analysis reveals rod/cone photoreceptor specific signatures across mammalian retinas. Hum. Mol. Genet. 25:4376–4388, 2016.

210.

Lakkaraju

, Umapathy

, Tan

L.X.

, Daniele

, Philp

N.J.

, Boesze-Battaglia

, and Williams

D.S.

The cell biology of the retinal pigment epithelium. Prog. Retin. Eye Res. 2020 [Epub ahead of print]; DOI: 10.1016/j.preteyeres.2020.100846.

211.

Bennis

, Gorgels

T.G.

, Ten Brink

J.B.

, van der Spek

P.J.

, Bossers

, Heine

V.M.

, and Bergen

A.A.

Comparison of mouse and human retinal pigment epithelium gene expression profiles: potential implications for age-related macular degeneration. PLoS One. 10:e0141597, 2015.

212.

Demetrius

Of mice and men. When it comes to studying ageing and the means to slow it down, mice are not just small humans. EMBO Rep. 6 Spec No:S39–S44, 2005.

213.

Kanow

M.A.

, Giarmarco

M.M.

, Jankowski

C.S.

, Tsantilas

, Engel

A.L.

, Du

, Linton

J.D.

, Farnsworth

C.C.

, Sloat

S.R.

, Rountree

, Sweet

I.R.

, Lindsay

K.J.

, Parker

E.D.

, Brockerhoff

S.E.

, Sadilek

, Chao

J.R.

, and Hurley

J.B.

Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye. Elife. 6: e28899, 2017.

214.

Zheng

, Reem

R.E.

, Omarova

, Huang

, DiPatre

P.L.

, Charvet

C.D.

, Curcio

C.A.

, and Pikuleva

I.A.

Spatial distribution of the pathways of cholesterol homeostasis in human retina. PLoS One. 7:e37926, 2012.

215.

Zheng

, Mast

, Saadane

, and Pikuleva

I.A.

Pathways of cholesterol homeostasis in mouse retina responsive to dietary and pharmacologic treatments. J. Lipid Res. 56:81–97, 2015.

216.

Hogarth

C.A.

, Roy

, and Ebert

D.L.

Genomic evidence for the absence of a functional cholesteryl ester transfer protein gene in mice and rats. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 135:219–229, 2003.

217.

Charles

M.A.

, and Kane

J.P.

New molecular insights into CETP structure and function: a review. J. Lipid Res. 53:1451–1458, 2012.

218.

Gordon

S.M.

, Li

, Zhu

, Shah

A.S.

, Lu

L.J.

, and Davidson

W.S.

A comparison of the mouse and human lipoproteome: suitability of the mouse model for studies of human lipoproteins. J. Proteome Res. 14:2686–2695, 2015.

219.

Bergen

W.G.

, and Mersmann

H.J.

Comparative aspects of lipid metabolism: impact on contemporary research and use of animal models. J. Nutr. 135:2499–2502, 2005.

220.

Adijanto

, and Philp

N.J.

Cultured primary human fetal retinal pigment epithelium (hfRPE) as a model for evaluating RPE metabolism. Exp. Eye Res. 126:77–84, 2014.

221.

Smith

E.N.

, D'Antonio-Chronowska

, Greenwald

W.W.

, Borja

, Aguiar

L.R.

, Pogue

, Matsui

, Benaglio

, Borooah

, D'Antonio

, Ayyagari

, and Frazer

K.A.

Human iPSC-derived retinal pigment epithelium: a model system for prioritizing and functionally characterizing causal variants at AMD risk loci. Stem Cell Reports. 12:1342–1353, 2019.

222.

Toops

K.A.

, Tan

L.X.

, and Lakkaraju

A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers. Exp. Eye Res. 124:74–85, 2014.

223.

Vielle

, Park

Y.K.

, Secora

, and Vergara

M.N.

Organoids for the study of retinal development and developmental abnormalities. Front. Cell. Neurosci. 15:667880, 2021.

224.

Zhao

, Chen

, Wang

, Sternberg

, Freeman

M.L.

, Grossniklaus

H.E.

, and Cai

Age-related retinopathy in NRF2-deficient mice. PLoS One. 6:e19456, 2011.

225.

Rosenfeld

P.J.

, Berger

, Reichel

, Danis

R.P.

, Gress

, Ye

, Magee

, Parham

L.R.

, and McLaughlin

M.M.

A Randomized phase 2 study of an anti-amyloid β monoclonal antibody in geographic atrophy secondary to age-related macular degeneration. Ophthalmol. Retina. 2:1028–1040, 2018.

226.

Edwards

A.O.

, Ritter

, 3rd, Abel

K.J.

, Manning

, Panhuysen

, and Farrer

L.A.

Complement factor H polymorphism and age-related macular degeneration. Science. 308:421–424, 2005.

227.

Haines

J.L.

, Hauser

M.A.

, Schmidt

, Scott

W.K.

, Olson

L.M.

, Gallins

, Spencer

K.L.

, Kwan

S.Y.

, Noureddine

, Gilbert

J.R.

, Schnetz-Boutaud

, Agarwal

, Postel

E.A.

, and Pericak-Vance

M.A.

Complement factor H variant increases the risk of age-related macular degeneration. Science. 308:419–421, 2005.

228.

Hageman

G.S.

, Anderson

D.H.

, Johnson

L.V.

, Hancox

L.S.

, Taiber

A.J.

, Hardisty

L.I.

, Hageman

J.L.

, Stockman

H.A.

, Borchardt

J.D.

, Gehrs

K.M.

, Smith

R.J.

, Silvestri

, Russell

S.R.

, Klaver

C.C.

, Barbazetto

, Chang

, Yannuzzi

L.A.

, Barile

G.R.

, Merriam

J.C.

, Smith

R.T.

, Olsh

A.K.

, Bergeron

, Zernant

, Merriam

J.E.

, Gold

, Dean

, and Allikmets

A common haplotype in the complement regulatory gene factor H (HF1/CFH) predisposes individuals to age-related macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 102:7227–7232, 2005.

229.

Klein

R.J.

, Zeiss

, Chew

E.Y.

, Tsai

J.Y.

, Sackler

R.S.

, Haynes

, Henning

A.K.

, SanGiovanni

J.P.

, Mane

S.M.

, Mayne

S.T.

, Bracken

M.B.

, Ferris

F.L.

, Ott

, Barnstable

, and Hoh

Complement factor H polymorphism in age-related macular degeneration. Science. 308:385–389, 2005.

230.

DiScipio

R.G.

Ultrastructures and interactions of complement factors H and I. J. Immunol. 149:2592–2599, 1992.

231.

Sharma

A.K.

, and Pangburn

M.K.

Identification of three physically and functionally distinct binding sites for C3b in human complement factor H by deletion mutagenesis. Proc. Natl. Acad. Sci. U. S. A. 93:10996–11001, 1996.

232.

Pickering

M.C.

, Cook

H.T.

, Warren

, Bygrave

A.E.

, Moss

, Walport

M.J.

, and Botto

Uncontrolled C3 activation causes membranoproliferative glomerulonephritis in mice deficient in complement factor H. Nat. Genet. 31:424–428, 2002.

233.

Vergnes

, Phan

, Strauss

, Tafuri

, and Reue

Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression. J. Biol. Chem. 278:42774–42784, 2003.

234.

Hoh Kam

, Lenassi

, Malik

T.H.

, Pickering

M.C.

, and Jeffery

Complement component C3 plays a critical role in protecting the aging retina in a murine model of age-related macular degeneration. Am. J. Pathol. 183:480–492, 2013.

235.

Faber

, Williams

, Juel

H.B.

, Greenwood

, Nissen

M.H.

, and Moss

S.E.

Complement factor H deficiency results in decreased neuroretinal expression of Cd59a in aged mice. Invest. Ophthalmol. Vis. Sci. 53:6324–6330, 2012.

236.

Wang

, Li

C.M.

, Rudolf

, Belyaeva

O.V.

, Chung

B.H.

, Messinger

J.D.

, Kedishvili

N.Y.

, and Curcio

C.A.

Lipoprotein particles of intraocular origin in human Bruch membrane: an unusual lipid profile. Invest. Ophthalmol. Vis. Sci. 50:870–877, 2009.

237.

C.M.

, Presley

J.B.

, Zhang

, Dashti

, Chung

B.H.

, Medeiros

N.E.

, Guidry

, and Curcio

C.A.

Retina expresses microsomal triglyceride transfer protein: implications for age-related maculopathy. J. Lipid Res. 46:628–640, 2005.

238.

Lyssenko

N.N.

, Haider

, Picataggi

, Cipollari

, Jiao

, Phillips

M.C.

, Rader

D.J.

, and Chavali

V.R.M.

Directional ABCA1-mediated cholesterol efflux and apoB-lipoprotein secretion in the retinal pigment epithelium. J. Lipid Res. 59:1927–1939, 2018.

239.

Savarese

, De Ferrari

G.M.

, Rosano

G.M.

, and Perrone-Filardi

Safety and efficacy of ezetimibe: a meta-analysis. Int. J. Cardiol. 201:247–252, 2015.

240.

Kelly

, Yu

, Kumar

, Ding

J.D.

, Jiang

, Hageman

G.S.

, Arshavsky

V.Y.

, Frank

M.M.

, Hauser

M.A.

, and Rickman

C.B.

Heparan sulfate, including that in Bruch's membrane, inhibits the complement alternative pathway: implications for age-related macular degeneration. J. Immunol. 185:5486–5494, 2010.

241.

Rudolf

, Curcio

C.A.

, Schlötzer-Schrehardt

, Sefat

A.M.M.

, Tura

, Aherrahrou

, Brinkmann

, Grisanti

, Miura

, and Ranjbar

Apolipoprotein A-I mimetic peptide L-4F removes Bruch's membrane lipids in aged nonhuman primates. Invest. Ophthalmol. Vis. Sci. 60:461–472, 2019.

242.

Raghow

, Yellaturu

, Deng

, Park

E.A.

, and Elam

M.B.

SREBPs: the crossroads of physiological and pathological lipid homeostasis. Trends Endocrinol. Metab. 19:65–73, 2008.

243.

Wang

, and Tontonoz

Liver X receptors in lipid signalling and membrane homeostasis. Nat. Rev. Endocrinol. 14:452–463, 2018.

244.

Ory

D.S.

Nuclear receptor signaling in the control of cholesterol homeostasis: have the orphans found a home?. Circ. Res. 95:660–670, 2004.

245.

Varga

, Czimmerer

, and Nagy

PPARs are a unique set of fatty acid regulated transcription factors controlling both lipid metabolism and inflammation. Biochim. Biophys. Acta. 1812:1007–1022, 2011.

246.

Farese

R.V.

, Véniant

M.M.

, Cham

C.M.

, Flynn

L.M.

, Pierotti

, Loring

J.F.

, Traber

, Ruland

, Stokowski

R.S.

, Huszar

, and Young

S.G.

Phenotypic analysis of mice expressing exclusively apolipoprotein B48 or apolipoprotein B100. Proc. Natl. Acad. Sci. U. S. A. 93:6393–6398, 1996.

247.

Dikic

, and Elazar

Mechanism and medical implications of mammalian autophagy. Nat. Rev. Mol. Cell. Biol. 19:349–364, 2018.

248.

Mitter

S.K.

, Song

, Qi

, Mao

, Rao

, Akin

, Lewin

, Grant

, Dunn

, Ding

, Bowes Rickman

, and Boulton

Dysregulated autophagy in the RPE is associated with increased susceptibility to oxidative stress and AMD. Autophagy. 10:1989–2005, 2014.

249.

Dhingra

, Sharp

R.C.

, Kim

, Popov

A.V.

, Ying

G.S.

, Pietrofesa

R.A.

, Park

, Christofidou-Solomidou

, and Boesze-Battaglia

Assessment of a small molecule synthetic lignan in enhancing oxidative balance and decreasing lipid accumulation in human retinal pigment epithelia. Int. J. Mol. Sci. 22: 5764, 2021.

250.

Zhang

, Presswalla

, Ali

R.R.

, Zacks

D.N.

, Thompson

D.A.

, and Miller

J.M.L.

Pharmacologic activation of autophagy without direct mTOR inhibition as a therapeutic strategy for treating dry macular degeneration. Aging (Albany NY). 13:10866–10890, 2021.

251.

Houten

S.M.

, Violante

, Ventura

F.V.

, and Wanders

R.J.

The biochemistry and physiology of mitochondrial fatty acid β-oxidation and its genetic disorders. Annu. Rev. Physiol. 78:23–44, 2016.

252.

Fleury

, Mignotte

, and Vayssiere

J.L.

Mitochondrial reactive oxygen species in cell death signaling. Biochimie. 84:131–141, 2002.

253.

Seo

S.J.

, Krebs

M.P.

, Mao

, Jones

, Conners

, and Lewin

A.S.

Pathological consequences of long-term mitochondrial oxidative stress in the mouse retinal pigment epithelium. Exp. Eye Res. 101:60–71, 2012.

254.

, Ma

H.Y.

, Park

, Ortogero

, Song

, Hennig

G.W.

, Zheng

, Lin

Y.M.

, Moro

, Hsieh

J.T.

, and Yan

The mitochondrial genome encodes abundant small noncoding RNAs. Cell Res. 23:759–774, 2013.

255.

Zhao

, Yasumura

, Li

, Matthes

, Lloyd

, Nielsen

, Ahern

, Snyder

, Bok

, Dunaief

J.L.

, LaVail

M.M.

, and Vollrath

mTOR-mediated dedifferentiation of the retinal pigment epithelium initiates photoreceptor degeneration in mice. J. Clin. Invest. 121:369–383, 2011.

256.

Rosales

M.A.B.

, Shu

D.Y.

, Iacovelli

, and Saint-Geniez

Loss of PGC-1α in RPE induces mesenchymal transition and promotes retinal degeneration. Life Sci. Alliance. 2:1–17, 2019.

257.

Liu

G.Y.

, and Sabatini

D.M.

mTOR at the nexus of nutrition, growth, ageing and disease. Nat. Rev. Mol. Cell Biol. 21:183–203, 2020.

258.

Huang

, Gu

, Chen

, Zhang

S.J.

, Jiang

, Chen

, Jiang

, Liu

, Radu

R.A.

, Sun

, Vollrath

, Du

, Yan

, and Zhao

Abnormal mTORC1 signaling leads to retinal pigment epithelium degeneration. Theranostics. 9:1170–1180, 2019.

259.

Rajala

, Wang

, and Rajala

R.V.S.

Constitutive activation mutant mTOR promote cone survival in retinitis pigmentosa mice. Adv. Exp. Med. Biol. 1074:491–497, 2018.

260.

Lin

, Xiong

, and Yang

Ribosomal protein S6 kinase 1 promotes the survival of photoreceptors in retinitis pigmentosa. Cell Death Dis. 9:1141, 2018.

261.

Venkatesh

, Ma

, Le

Y.Z.

, Hall

M.N.

, Rüegg

M.A.

, and Punzo

Activated mTORC1 promotes long-term cone survival in retinitis pigmentosa mice. J. Clin. Invest. 125:1446–1458, 2015.

262.

Punzo

, Kornacker

, and Cepko

C.L.

Stimulation of the insulin/mTOR pathway delays cone death in a mouse model of retinitis pigmentosa. Nat. Neurosci. 12:44–52, 2009.

263.

Yagasaki

, Nakahara

, Mori

, Sakamoto

, and Ishii

Effects of mTOR inhibition on normal retinal vascular development in the mouse. Exp. Eye Res. 129:127–134, 2014.

264.

Morita

, Gravel

S.P.

, Hulea

, Larsson

, Pollak

, St-Pierre

, and Topisirovic

mTOR coordinates protein synthesis, mitochondrial activity and proliferation. Cell Cycle. 14:473–480, 2015.

265.

Starr

C.R.

, Nyankerh

C.N.A.

, Qi

, Hu

, Gorbatyuk

O.S.

, Sonenberg

, Boulton

M.E.

, and Gorbatyuk

M.S.

Role of translational attenuation in inherited retinal degeneration. Invest. Ophthalmol. Vis. Sci. 60:4849–4857, 2019.

266.

Molday

L.L.

, Rabin

A.R.

, and Molday

R.S.

ABCR expression in foveal cone photoreceptors and its role in Stargardt macular dystrophy. Nat. Genet. 25:257–258, 2000.

267.

Illing

, Molday

L.L.

, and Molday

R.S.

The 220-kDa rim protein of retinal rod outer segments is a member of the ABC transporter superfamily. J. Biol. Chem. 272:10303–10310, 1997.

268.

Sun

, and Nathans

Stargardt's ABCR is localized to the disc membrane of retinal rod outer segments. Nat. Genet. 17:15–16, 1997.

269.

Tsybovsky

, Molday

R.S.

, and Palczewski

The ATP-binding cassette transporter ABCA4: structural and functional properties and role in retinal disease. Adv. Exp. Med. Biol. 703:105–125, 2010.

270.

Kim

S.R.

, Jang

Y.P.

, Jockusch

, Fishkin

N.E.

, Turro

N.J.

, and Sparrow

J.R.

The all-trans-retinal dimer series of lipofuscin pigments in retinal pigment epithelial cells in a recessive Stargardt disease model. Proc. Natl. Acad. Sci. U. S. A. 104:19273–19278, 2007.

271.

Mata

N.L.

, Weng

, and Travis

G.H.

Biosynthesis of a major lipofuscin fluorophore in mice and humans with ABCR-mediated retinal and macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 97:7154–7159, 2000.

272.

Anderson

D.M.G.

, Ablonczy

, Koutalos

, Hanneken

A.M.

, Spraggins

J.M.

, Calcutt

M.W.

, Crouch

R.K.

, Caprioli

R.M.

, and Schey

K.L.

Bis(monoacylglycero)phosphate lipids in the retinal pigment epithelium implicate lysosomal/endosomal dysfunction in a model of Stargardt disease and human retinas. Sci. Rep. 7:17352, 2017.

273.

Hullin-Matsuda

, Luquain-Costaz

, Bouvier

, and Delton-Vandenbroucke

Bis(monoacylglycero)phosphate, a peculiar phospholipid to control the fate of cholesterol: implications in pathology. Prostaglandins Leukot. Essent. Fatty Acids. 81:313–324, 2009.

274.

Kaur

, Tan

L.X.

, Rathnasamy

, La Cunza

, Germer

C.J.

, Toops

K.A.

, Fernandes

, Blenkinsop

T.A.

, and Lakkaraju

Aberrant early endosome biogenesis mediates complement activation in the retinal pigment epithelium in models of macular degeneration. Proc. Natl. Acad. Sci. U. S. A. 115:9014–9019, 2018.

275.

Logan

, and Anderson

R.E.

Dominant stargardt macular dystrophy (STGD3) and ELOVL4. Adv. Exp. Med. Biol. 801:447–453, 2014.

276.

Logan

, Agbaga

M.P.

, Chan

M.D.

, Kabir

, Mandal

N.A.

, Brush

R.S.

, and Anderson

R.E.

Deciphering mutant ELOVL4 activity in autosomal-dominant Stargardt macular dystrophy. Proc. Natl. Acad. Sci. U. S. A. 110:5446–5451, 2013.

277.

, Benham

, Logan

, Brush

R.S.

, Mandal

M.N.

, Anderson

R.E.

, and Agbaga

M.P.

ELOVL4 protein preferentially elongates 20:5n3 to very long chain PUFAs over 20:4n6 and 22:6n3. J. Lipid Res. 53:494–504, 2012.

278.

Choi

, Gorusupudi

, and Bernstein

P.S.

Long-term follow-up of autosomal dominant Stargardt macular dystrophy (STGD3) subjects enrolled in a fish oil supplement interventional trial. Ophthalmic Genet. 39:307–313, 2018.

279.

, Marchette

L.D.

, Brush

R.S.

, Elliott

M.H.

, Le

Y.Z.

, Henry

K.A.

, Anderson

A.G.

, Zhao

, Sun

, Zhang

, and Anderson

R.E.

DHA does not protect ELOVL4 transgenic mice from retinal degeneration. Mol. Vis. 15:1185–1193, 2009.

280.

Dornstauder

, Suh

, Kuny

, Gaillard

, Macdonald

I.M.

, Clandinin

M.T.

, and Sauvé

Dietary docosahexaenoic acid supplementation prevents age-related functional losses and A2E accumulation in the retina. Invest. Ophthalmol. Vis. Sci. 53:2256–2265, 2012.

281.

Harkewicz

, Du

, Tong

, Alkuraya

, Bedell

, Sun

, Wang

, Hsu

Y.H.

, Esteve-Rudd

, Hughes

, Su

, Zhang

, Lopes

V.S.

, Molday

R.S.

, Williams

D.S.

, Dennis

E.A.

, and Zhang

Essential role of ELOVL4 protein in very long chain fatty acid synthesis and retinal function. J. Biol. Chem. 287:11469–11480, 2012.

282.

Age-Related Eye Disease Study 2 Research Group. Lutein + zeaxanthin and omega-3 fatty acids for age-related macular degeneration: the Age-Related Eye Disease Study 2 (AREDS2) randomized clinical trial. JAMA. 309:2005–2015, 2013.

283.

Gordon

D.J.

, Probstfield

J.L.

, Garrison

R.J.

, Neaton

J.D.

, Castelli

W.P.

, Knoke

J.D.

, Jacobs

D.R.

, Bangdiwala

, and Tyroler

H.A.

High-density lipoprotein cholesterol and cardiovascular disease. Four prospective American studies. Circulation. 79:8–15, 1989.

284.

Fisher

E.A.

, Feig

J.E.

, Hewing

, Hazen

S.L.

, and Smith

J.D.

High-density lipoprotein function, dysfunction, and reverse cholesterol transport. Arterioscler. Thromb. Vasc. Biol. 32:2813–2820, 2012.

285.

Navab

, Shechter

, Anantharamaiah

G.M.

, Reddy

S.T.

, Van Lenten

B.J.

, and Fogelman

A.M.

Structure and function of HDL mimetics. Arterioscler. Thromb. Vasc. Biol. 30:164–168, 2010.

286.

Watson

C.E.

, Weissbach

, Kjems

, Ayalasomayajula

, Zhang

, Chang

, Navab

, Hama

, Hough

, Reddy

S.T.

, Soffer

, Rader

D.J.

, Fogelman

A.M.

, and Schecter

Treatment of patients with cardiovascular disease with L-4F, an apo-A1 mimetic, did not improve select biomarkers of HDL function. J. Lipid Res. 52:361–373, 2011.

287.

Zheng

K.H.

, Kaiser

, van Olden

C.C.

, Santos

R.D.

, Dasseux

J.L.

, Genest

, Gaudet

, Westerink

, Keyserling

, Verberne

H.J.

, Leitersdorf

, Hegele

R.A.

, Descamps

O.S.

, Hopkins

, Nederveen

A.J.

, and Stroes

E.S.G.

No benefit of HDL mimetic CER-001 on carotid atherosclerosis in patients with genetically determined very low HDL levels. Atherosclerosis. 311:13–19, 2020.

288.

Tardif

J.C.

, Ballantyne

C.M.

, Barter

, Dasseux

J.L.

, Fayad

Z.A.

, Guertin

M.C.

, Kastelein

J.J.

, Keyserling

, Klepp

, Koenig

, L'Allier

P.L.

, Lespérance

, Lüscher

T.F.

, Paolini

J.F.

, Tawakol

, Waters

D.D.

, and Can HDL Infusions Significantly QUicken Atherosclerosis REgression (CHI-SQUARE) Investigators. Effects of the high-density lipoprotein mimetic agent CER-001 on coronary atherosclerosis in patients with acute coronary syndromes: a randomized trial. Eur. Heart J. 35:3277–3286, 2014.

289.

Dunbar

R.L.

, Movva

, Bloedon

L.T.

, Duffy

, Norris

R.B.

, Navab

, Fogelman

A.M.

, and Rader

D.J.

Oral apolipoprotein A-I mimetic D-4F lowers HDL-inflammatory index in high-risk patients: a first-in-human multiple-dose, randomized controlled trial. Clin. Transl. Sci. 10:455–469, 2017.

290.

Rudolf

, Mir Mohi Sefat

, Miura

, Tura

, Raasch

, Ranjbar

, Grisanti

, Aherrahrou

, Wagner

, Messinger

J.D.

, Garber

D.W.

, Anantharamaiah

G.M.

, and Curcio

C.A.

ApoA-I mimetic peptide 4F reduces age-related lipid deposition in murine bruch's membrane and causes its structural remodeling. Curr. Eye Res. 43:135–146, 2018.

291.

, Spee

, Araujo

, Barron

, Wang

, Ghione

, Hinton

D.R.

, Nusinowitz

, Kannan

, Reddy

S.T.

, and Farias-Eisner

A novel HDL-mimetic peptide HM-10/10 protects RPE and photoreceptors in murine models of retinal degeneration. Int. J. Mol. Sci. 20: 4807, 2019.

292.

, Zhang

, Gao

, Fariss

R.N.

, Tam

, and Wong

W.T.

Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury. Sci. Rep. 7:8433, 2017.

293.

, Roubeix

, Sennlaub

, and Saban

D.R.

Microglia versus monocytes: distinct roles in degenerative diseases of the retina. Trends Neurosci. 43:433–449, 2020.

294.

Zhou

, and Liao

J.K.

Statins and cardiovascular diseases: from cholesterol lowering to pleiotropy. Curr. Pharm. Des. 15:467–478, 2009.

295.

Guymer

R.H.

, Baird

P.N.

, Varsamidis

, Busija

, Dimitrov

P.N.

, Aung

K.Z.

, Makeyeva

G.A.

, Richardson

A.J.

, Lim

, and Robman

L.D.

Proof of concept, randomized, placebo-controlled study of the effect of simvastatin on the course of age-related macular degeneration. PLoS One. 8:e83759, 2013.

296.

Vavvas

D.G.

, Daniels

A.B.

, Kapsala

Z.G.

, Goldfarb

J.W.

, Ganotakis

, Loewenstein

J.I.

, Young

L.H.

, Gragoudas

E.S.

, Eliott

, Kim

I.K.

, Tsilimbaris

M.K.

, and Miller

J.W.

Regression of some high-risk features of age-related macular degeneration (AMD) in patients receiving intensive statin treatment. EBioMedicine. 5:198–203, 2016.

297.

Barbosa

D.T.

, Mendes

T.S.

, Cíntron-Colon

H.R.

, Wang

S.Y.

, Bhisitkul

R.B.

, Singh

, and Lin

S.C.

Age-related macular degeneration and protective effect of HMG Co-A reductase inhibitors (statins): results from the National Health and Nutrition Examination Survey 2005–2008. Eye (Lond). 28:472–480, 2014.

298.

McGwin

, Xie

, and Owsley

The use of cholesterol-lowering medications and age-related macular degeneration. Ophthalmology. 112:488–494, 2005.

299.

Wilson

H.L.

, Schwartz

D.M.

, Bhatt

H.R.

, McCulloch

C.E.

, and Duncan

J.L.

Statin and aspirin therapy are associated with decreased rates of choroidal neovascularization among patients with age-related macular degeneration. Am. J. Ophthalmol. 137:615–624, 2004.

300.

Rajeshuni

, Ludwig

C.A.

, and Moshfeghi

D.M.

The effect of statin exposure on choroidal neovascularization in nonexudative age-related macular degeneration patients. Eye (Lond). 33:163–165, 2019.

301.

VanderBeek

B.L.

, Zacks

D.N.

, Talwar

, Nan

, and Stein

J.D.

Role of statins in the development and progression of age-related macular degeneration. Retina. 33:414–422, 2013.

302.

Lee

, Jeon

H.L.

, Park

S.J.

, and Shin

J.Y.

Effect of statins, metformin, angiotensin-converting enzyme inhibitors, and angiotensin II receptor blockers on age-related macular degeneration. Yonsei Med. J. 60:679–686, 2019.

303.

Al-Holou

S.N.

, Tucker

W.R.

, Agrón

, Clemons

T.E.

, Cukras

, Ferris

F.L.

, Chew

E.Y.

, and Age-Related Eye Disease Study 2 Research

Group.

The association of statin use with age-related macular degeneration progression: the age-related eye disease study 2 report number 9. Ophthalmology. 122:2490–2496, 2015.

304.

Mandas

, Mereu

R.M.

, Catte

, Saba

, Serchisu

, Costaggiu

, Peiretti

, Caminiti

, Vinci

, Casu

, Piludu

, Fossarello

, Manconi

P.E.

, and Dessí

Cognitive impairment and age-related vision disorders: their possible relationship and the evaluation of the use of aspirin and statins in a 65 years-and-over sardinian population. Front. Aging Neurosci. 6:309, 2014.

305.

Klein

, Myers

C.E.

, Buitendijk

G.H.

, Rochtchina

, Gao

, de Jong

P.T.

, Sivakumaran

T.A.

, Burlutsky

, McKean-Cowdin

, Hofman

, Iyengar

S.K.

, Lee

K.E.

, Stricker

B.H.

, Vingerling

J.R.

, Mitchell

, Klein

B.E.

, Klaver

C.C.

, and Wang

J.J.

Lipids, lipid genes, and incident age-related macular degeneration: the three continent age-related macular degeneration consortium. Am. J. Ophthalmol. 158:513–524.e513, 2014.

306.

Shalev

, Sror

, Goldshtein

, Kokia

, and Chodick

Statin use and the risk of age related macular degeneration in a large health organization in Israel. Ophthalmic Epidemiol. 18:83–90, 2011.

307.

Fong

D.S.

, and Contreras

Recent statin use and 1-year incidence of exudative age-related macular degeneration. Am. J. Ophthalmol. 149:955–958.e951, 2010.

308.

Maguire

M.G.

, Ying

G.S.

, McCannel

C.A.

, Liu

, Dai

, and Complications of Age-related Macular Degeneration Prevention Trial (CAPT) Research

Group.

Statin use and the incidence of advanced age-related macular degeneration in the Complications of Age-related Macular Degeneration Prevention Trial. Ophthalmology. 116:2381–2385, 2009.

309.

Kaiserman

, Vinker

, and Kaiserman

Statins do not decrease the risk for wet age-related macular degeneration. Curr. Eye Res. 34:304–310, 2009.

310.

Klein

, Knudtson

M.D.

, and Klein

B.E.

Statin use and the five-year incidence and progression of age-related macular degeneration. Am. J. Ophthalmol. 144:1–6, 2007.

311.

Tan

J.S.

, Mitchell

, Rochtchina

, and Wang

J.J.

Statins and the long-term risk of incident age-related macular degeneration: the Blue Mountains Eye Study. Am. J. Ophthalmol. 143:685–687, 2007.

312.

McGwin

, Modjarrad

, Hall

T.A.

, Xie

, and Owsley

3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitors and the presence of age-related macular degeneration in the Cardiovascular Health Study. Arch. Ophthalmol. 124:33–37, 2006.

313.

Smeeth

, Cook

, Chakravarthy

, Hubbard

, and Fletcher

A.E.

A case control study of age related macular degeneration and use of statins. Br. J. Ophthalmol. 89:1171–1175, 2005.

314.

Klein

, Klein

B.E.

, Tomany

S.C.

, Danforth

L.G.

, and Cruickshanks

K.J.

Relation of statin use to the 5-year incidence and progression of age-related maculopathy. Arch. Ophthalmol. 121:1151–1155, 2003.

315.

Mast

, Bederman

I.R.

, and Pikuleva

I.A.

Retinal cholesterol content is reduced in simvastatin-treated mice due to inhibited local biosynthesis albeit increased uptake of serum cholesterol. Drug Metab. Dispos. 46:1528–1537, 2018.

316.

Ryeom

S.W.

, Sparrow

J.R.

, and Silverstein

R.L.

CD36 participates in the phagocytosis of rod outer segments by retinal pigment epithelium. J. Cell Sci. 109: 387–395, 1996.

317.

Yamada

, Tian

, Yang

, Cutler

R.G.

, Wu

, Telljohann

R.S.

, Mattson

M.P.

, and Handa

J.T.

Oxidized low density lipoproteins induce a pathologic response by retinal pigmented epithelial cells. J. Neurochem. 105:1187–1197, 2008.

318.

Yin

, Wu

, Gong

, Shi

, Qiu

, Zhang

, Liu

, and Gu

OX-LDL up-regulates the vascular endothelial growth factor-to-pigment epithelium-derived factor ratio in human retinal pigment epithelial cells. Curr. Eye Res. 36:379–385, 2011.

319.

Ebrahimi

K.B.

, Fijalkowski

, Cano

, and Handa

J.T.

Decreased membrane complement regulators in the retinal pigmented epithelium contributes to age-related macular degeneration. J. Pathol. 229:729–742, 2013.

320.

Gnanaguru

, Choi

A.R.

, Amarnani

, and D'Amore

P.A.

Oxidized lipoprotein uptake through the CD36 receptor activates the NLRP3 inflammasome in human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 57:4704–4712, 2016.

321.

A.L.

, Lorenz

R.L.

, Haritoglou

, Kampik

, and Welge-Lussen

Biological effects of native and oxidized low-density lipoproteins in cultured human retinal pigment epithelial cells. Exp. Eye Res. 88:495–503, 2009.

322.

Yating

, Yuan

, Wei

, Qing

, Xingwei

, Qiu

, and Lili

Oxidized LDL induces apoptosis of human retinal pigment epithelium through activation of ERK-Bax/Bcl-2 signaling pathways. Curr. Eye Res. 40:415–422, 2015.

Targeting Lipid Metabolism for the Treatment of Age-Related Macular Degeneration: Insights from Preclinical Mouse Models

Abstract

Introduction

Associations of Dysregulated Lipid Metabolism with AMD

Models of AMD

Limitations of Mouse Models in AMD and Lipid Metabolic Research

Differences between mouse and human retinas

Differences between mouse and human lipid metabolism

Translatability of mouse studies to the clinic

Devising and Testing Lipid Metabolism-Targeting Therapies in Preclinical AMD Mouse Models

Clearance of pathogenic lipid or protein components in Sub-RPE deposits

Restoration of lipid processing in the RPE and BrM

Preservation of lipid oxidative pathways

Lipid Metabolism-Targeting Therapies Tested in Preclinical AMD Mouse Models

Despiramine and TO901316

DHA treatment

Apolipoprotein mimetics

Statins

Conclusion

Footnotes

Acknowledgments

Author Disclosure Statement

Funding Information

References