Abstract
Purpose:
The aim of this study was to determine the distribution and concentrations of bromfenac ophthalmic solution in ocular tissues following topical instillation in New Zealand White (NZW) rabbits.
Design:
Two animal studies were conducted.
Methods:
A single 50-μL 14C-bromfenac ophthalmic solution (20–25 μCi or 0.09%) was administered into the right eyes of 14–18 randomly assigned NZW rabbits. At various time points, ocular tissues were collected and analyzed for 14C-bromfenac contents. Ocular tissues were combusted and the amount of radioactivity was determined by liquid scintillation counting (LSC). Aqueous-humor samples were directly transferred to LSC vials.
Results:
Peak concentrations of 14C-bromfenac were observed in the aqueous humor and most ocular tissues at or before 2-hours. The highest concentrations were in the cornea, conjunctiva, and sclera. Similar amounts were detected in the aqueous humor, iris-ciliary body, choroid, and, to a slightly lesser degree, in the retina. Measurable amounts of bromfenac were detected in all samples at the 24-hours time point (≥0.001 μg equivalent/g).
Conclusions:
Significant penetration and measurable amounts of 14C-bromfenac were detected in all ocular tissues over 24 h, including the sclera, choroid, and retina. These results strongly suggest the utility of bromfenac ophthalmic solution 0.09% in treating inflammation of both the anterior and posterior ocular segments.
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