Abstract
ABSTRACT
Acini cells were obtained from the lacrimal gland of the white New Zealand rabbit. Following isolation and purification, the cells were used to study the uptake of N,N'-dimethyl-2-phenylethylamine HCl (AF2975), which was found to be sodium- and proton-independent, but energy-dependent. Uptake was mainly accomplished via a carrier-mediated transport system for which a Km of 8.72 ± 0.96 mM, a Vmax of 602.6 ± 41.3 nmol/mg of protein/min, and an exponential coefficient of 2.55 ± 0.46 were obtained following a least squares nonlinear fit to the Hill equation. With the addition of the metabolic inhibitors, sodium azide or 2,4-dinitrophenol, the initial uptake rates were reduced from the control experiments by 35.7% and 26.2%, respectively.
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