Abstract
ABSTRACT
Nongpigmented epithelial (NPE) cells and pigmented epithelial (PE) cells were separated from porcine ciliary body by a simple surgical procedure. Using primary cultures of separated cells, basal and induced activities of 7-ethoxy resorufin (ER) O-dealkylase, 7-pentoxy resorufin (PR) O-dealkylase, and gamma-glutamyl transpeptidase (GGTP) were determined. These enzymatic activities were primarily associated with NPE cells; PE cells showed little or no activity. Treatment of NPE cells with phenobarbital for 48 hours resulted in about 5-fold increase in PR O-dealkylase activity but not ER-Odealkylase activity. Conversely, 3-methylcholanthrene treatment increased ER O-dealkylase activity 5-6 times over the basal level but had little or nor effect on PR O-dealkylase activity. The enhancement of enzymatic activities by inducers was completely inhibited by cyclohexamide. NPE cells exhibited higher GGTP activity than PE cells. Both phenobarbital and 3-methylcholanthrene augmented the GGTP activity of NPE cells but not of PE cells. The physiological significance of the regional differences in enzyme distribution was discussed.
Get full access to this article
View all access options for this article.