In this study, the flagella antigen was detached at high speed by shaking vigorously with glass pearl beads, from Salmonella enteritidis in a yield of 3.9 mg/mL−1 after being concentrated with polyethylene glycol (PEG). A monoclonal antibody (MAb), designated D7 clone, was generated from Balb/c mice immunized with Salmonella enteritidis flagella using the conventional hybridoma method. The D7 clone secreting MAb was classified as IgG2a isotype. ELISA analyses of D7 MAb to Salmonella-specific flagella demonstrated that the antibody reacted with H: m. However, Western immunoblot analyses of D7 clone appear to be secreting heavy chain of IgG2a antibody, which was eligible for the diagnosis of Salmonella enteritidis.
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