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Abstract

We have devised a method for the purification of human basophils from umbilical cord blood by FCM. Umbilical cord blood was collected from six healthy, full-term deliveries. After separation of red blood cells, mononuclear cells were isolated by Ficoll-Hypaque. Six samples followed by positive selection using flowcytometry (FCM) for CD203c⁺CD45^int+ cells. Purity and recovery of cells were measured. Purity and recovery of basophils by FCM with CD203c⁺CD45^int+ markers were 95.02 ± 2.94% and 61.42 ± 5.95%. Cell sorting for CD203c⁺CD45^int+ cells by FCM is an improved method for obtaining pure umbilical cord blood-derived basophils. We established cord blood-derived basophil purification technique as a source from which active basophils can be isolated for immunochemical characterization.

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Method for Umbilical Cord Blood-derived Basophils by FCM

Abstract

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