Monoclonal Antibodies That Recognize Trypsin Binding Domain of Human Urinary Trypsin Inhibitor

Abstract

Three MAb M2, B6, PI (IgG₁ type) against human urinary trypsin inhibitor (UTI), a glycoprotein with antiinflammatory properties, have been produced by hybridization of mouse myeloma cells P₃O₁ with spleen cells of immunized mice BALB/c. Competitive ELISA-examination of peroxidase conjugates of M₂, B₆, and P₁ MAb in the presence of the trypsin binding domain shows the highest affinity of M₂ antibody for this domain. On the basis of MAb M2 competitive ELISA of UTI concentration in urine is proposed. ELISA detectable changes in the UTI content of urine from patients with nephritis without renal failure can be considered as an early index of renal parenchyma damage.