Abstract
Three monoclonal antibodies (two IgG, and one IgA) prepared against the plant cytokinin, cis-zeatin riboside, were characterized for use in a fluorescence competition enzyme linked immunosorbant assay (F-ELISA). Immunoassays that employed each antibody detected femtomole quantities of the homologous cytokinin and characteristic quantities of structurally-related cytokinins. One of the antibodies was used to quantify HPLC-purified cis- zeatin riboside recovered from spiked samples of wheat leaves. These are the first monoclonal antibodies against a cis- derivative of a plant cytokinin and will be useful for purification and quantification of cis-zeatin and the 9-riboside and 9-glucoside in plant samples.
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