Abstract
A cloned mouse hybridoma was established that secreted a monoclonal antibody directed against certain neutral glycosphingolipids of the third-instar larvae of Calliphora vicina (Insecta: Diptera). The isotype of the designated monoclonal antibody CNF-I (Calliphora Neutral Fraction) was determined as IgG3. By the use of purified neutral glycosphingolipids of C.vicina, qualitative thin-layer chromatography immunostaining and semi-quantitative enzyme-linked immunosorbent assay determinations, the epitope was specified as a terminal α1-4-linked N-acetylgalactosamine to subterminal N-acetylgalactosamine, which is present on the components GalNAca4GalNAcß4GlcNAcß3Manß4GlcßlCer (N5a) and GalNAcα4GalNAcß4-(6' PEtn-)GlcNAcß3Manß4GlcßlCer (Nz5a). After enzymatic removal of the terminal α-N-acetylgalactosamine residue, the epitope reactivity was destroyed. The distribution of the CNF-I recognized epitope among neutral glycolipids of other insects was shown for: Locusta migratoria (Insecta: Orthoptera); and, Chironomus tentans and Drosophila melanogaster (Insecta: Diptera).
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