Abstract
β-galactosidase conjugated to erythrocyte vesicles was measured by enzymatic and immunologic methods. The immunoassay used a monoclonal antibody and an anti-mouse IgG antibody conjugated to alkaline phosphatase in an adaptation of the enzyme-linked immunosorbant assay. The β-galactosidase and alkaline phosphatase activities were compared by correlation. The significant correlation indicates that this method is satisfactory for relative quantitation of membrane-associated antigens with exposed epitopes.
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