Analysis of Hen Egg White Lysozyme (HEL)-Specific Delayed Type Hypersensitivity Hybridomas

Abstract

Hen egg white lysozyme (HEL) specific delayed type hypersensitivity hybridomas (TDTH hybridomas) were established by fusing HEL specific C3H/HeN (H-2^k haplotype) TDTH with BW5147 thymoma cells. TDTH hybridomas, which had Lyt-l⁺2^- and Thy-1.2 antigens on their surfaces, were injected into mouse footpads with HEL antigens, and foot pad swellings were measured 24 h later. Mice with I-A^k haplotype showed strong responses, and their swellings were reduced by anti-I-A^k specific monoclonal antibodies, which suggests that the activity of the hybridomas is restricted by I-A^k. However, TDTH hybridomas did not modulate HEL specific T-cell proliferation responses.