Restricted accessResearch articleFirst published online 2025-06
Investigating the Effect of Silver and Silica Nanoparticles on the Hemolysis of Human Blood Cells and the Survival of Jurkat Lymphocyte Cells in a Cell Culture Model
The growing global production and application of nanomaterials have brought nanotoxicology into the spotlight, particularly regarding their potential health risks. Despite these advancements, the potential adverse effects of these nanoparticles on human health remain largely underexplored.
Materials and Methods:
This study evaluated the toxicity of Silver nanoparticles (AgNPs) and silica nanoparticles (SiO2-NPs) on blood cells using two.
Methods:
Assessing cell viability in the Jurkat blood cell line via the MTT assay at nanoparticle concentrations ranging from 1 to 1000 μg/mL over 24, 48, and 72 hours, and examining hemolysis in peripheral blood using the Acid Citrate Dextrose method. IC50 values were determined through web-based programs and results were analyzed statistically.
Results:
The MTT assay revealed that the cytotoxic effects of AgNPs and SiO2-NPs varied based on their physicochemical properties, concentration, and duration of exposure. Hemolysis tests indicated that SiO2-NPs resulted in an increased percentage of hemolysis with rising concentrations. At lower concentrations, AgNPs induced a higher level of hemolysis compared with SiO2-NPs; however, at concentrations of 500 and 1000 μg/mL, SiO2-NPs exhibited significantly greater hemolytic activity.
Conclusion:
This study highlights important implications for biomedical applications, particularly in drug delivery and cancer therapy, by comparing the toxicity of AgNPs and SiO2-NPs. It emphasizes the necessity for a deeper understanding of toxicity mechanisms to effectively translate in vitro findings into clinical practice. Future research should focus on bridging the gap between in vitro and in vivo studies to ensure the safe application of these nanoparticles.
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