Sage Journals: Discover world-class research

Abstract

Most emerging infectious diseases today are arthropod-borne and cannot be prevented by vaccinations. Because insect repellents offer important topical barriers of personal protection from arthropod-borne infectious diseases, the main objectives of this article were to describe the growing threats to public health from emerging arthropod-borne infectious diseases, to define the differences between insect repellents and insecticides, and to compare the efficacies and toxicities of chemical and plant-derived insect repellents. Internet search engines were queried with key words to identify scientific articles on the efficacy, safety, and toxicity of chemical and plant-derived topical insect repellants and insecticides to meet these objectives. Data sources reviewed included case reports; case series; observational, longitudinal, and surveillance studies; and entomological and toxicological studies. Descriptive analysis of the data sources identified the most effective application of insect repellents as a combination of topical chemical repellents, either N-diethyl-3-methylbenzamide (formerly N, N-diethyl-m-toluamide, or DEET) or picaridin, and permethrin-impregnated or other pyrethroid-impregnated clothing over topically treated skin. The insecticide-treated clothing would provide contact-level insecticidal effects and provide better, longer lasting protection against malaria-transmitting mosquitoes and ticks than topical DEET or picaridin alone. In special cases, where environmental exposures to disease-transmitting ticks, biting midges, sandflies, or blackflies are anticipated, topical insect repellents containing IR3535, picaridin, or oil of lemon eucalyptus (p-menthane-3, 8-diol or PMD) would offer better topical protection than topical DEET alone.

Keywords

repellents insect insecticides infectious diseases arthropod-borne mosquito-borne tick-borne

Introduction

Most emerging infectious diseases today are arthropod-borne by ticks or mosquitoes and, with few exceptions, cannot be prevented by vaccinations. Lyme disease, transmitted by ixodid tick bites, is now the most common arthropod-borne infectious disease in the United States and Europe.¹ Recently, 3 new tick-borne diseases have been described in the United States: heartland virus disease, Borrelia miyamotoi borreliosis, and 364D rickettsiosis.² ^–6 An introduced species of mosquito in the United States and Europe, Aedes albopictus, has proven itself to be as competent a new vector of dengue and chikungunya viruses as Aedes aegypti, the yellow fever vector, has been in the tropics.^7,8 Because insect repellents offer important topical barriers of personal protection from arthropod-borne infectious diseases, the objectives of this article were to 1) describe the growing threats to public health from emerging arthropod-borne infectious diseases; 2) define the differences between insect repellents and insecticides; 3) compare the efficacies and toxicities of chemical and plant-derived insect repellents; 4) recommend the best combinations of insect repellents and insecticides for personal protection; and 5) describe the most effective nonchemical methods of personal protection from insect bites.

Methods

To meet the objectives of this article, Internet search engines including PubMed, Medline, Ovid, Google, Google Scholar, and Cochrane were queried with key words as medical subject headings to identify peer-reviewed scientific articles and governmental publications on the efficacy, safety, and toxicity of synthetic chemical and plant-derived topical insect repellants and insecticides. The key words included repellants, insect; insecticides; and infectious diseases, arthropod-borne, mosquito-borne, and tick-borne.

The articles selected to meet the first objective of describing the burden of arthropod-borne diseases included case reports and case series of newly described infectious diseases and observational, longitudinal, and surveillance studies. The articles selected to meet the second and third objectives of differentiating insecticides from repellents and comparing their efficacies and toxicities included entomological and toxicological studies and field and laboratory evaluations of different repellent and insecticidal formulations. The articles selected to meet the last 2 objectives to recommend the best combinations of chemical and nonchemical methods of personal protection from insect bites included both randomized controlled trials and U.S. governmental publications by the Centers for Disease Control and Prevention (CDC), Environmental Protection Agency (EPA), and the Food and Drug Administration (FDA). Because this investigation was review of prior published scientific articles, Institutional Review Board approval was waived.

Results

Definitions

An insect repellant is defined as a chemical or organic agent that makes the atmosphere within 4 centimeters of human skin so noxious to insects as to discourage contact and biting.⁹ On the other hand, an insecticide is a chemical or organic agent, often plant-derived, that kills insects, typically with a neurotoxin.⁹ Some insect repellents are also insecticides, such as plant-derived permethrin and all other synthetic pyrethroids.⁹

In the United States, the FDA tests and approves topical insect repellants, such as N, N-diethyl-3-methylbenzamide (formerly N, N-diethyl-m-toluamide or DEET) for use and safety in pregnant women; and the EPA approves insecticides for use under the Federal Insecticide, Fungicide, and Rodenticide Act. Many insecticides, such as the carbamates and organophosphates, are EPA-approved for outdoor use but not for indoor use. The only insecticides approved for indoor use are the pyrethroids, which are laboratory-made extracts derived from crushed, dried Chrysanthemum flowers.

Why Use Insect Repellents?

The 3 major reasons to use insect repellents are: 1) new threats to human health posed by emerging and imported arthropod-borne infectious diseases; 2) the dominance of new, competent insect vectors of infectious diseases; and 3) the inability to primarily prevent the transmission of most arthropod-borne infection diseases by vaccinations with the exceptions of yellow fever vaccine in South America and Africa, Japanese encephalitis vaccine in Southeast Asia, and several regional tick-borne virus vaccines in Eastern Europe.

The Threats from Emerging and Imported Arthropod-Borne Infectious Diseases

Arthropod-borne infectious diseases are primarily transmitted to humans from extensive zoonotic reservoirs in birds and mammals via the bites of infected mosquitoes, midges, flies, fleas, and ticks. The most common arthropod-borne disease in the United States and Europe today is tick-transmitted Lyme disease caused by the spirochete, Borrelia burgdorferi.¹ The most common mosquito-borne infectious disease in the United States today is West Nile virus (WNV), an arbovirus transmitted to humans by bites from Culicine mosquitoes.¹⁰ Although most cases of WNV infection remain asymptomatic, WNV neuroinvasive disease causes meningoencephalitis that often results in permanent neurological impairment.¹¹ After WNV, the next most commonly reported arboviral encephalitides in the United States include mosquito-borne LaCrosse virus, Jamestown Canyon virus, eastern equine encephalitis virus, and tick-borne Powassan virus, which has the highest case fatality rate among the arboviruses.¹⁰

Unlike mosquitoes, ticks are versatile insect vectors that can transmit a variety of pathogens, including bacteria, viruses, and parasites. In addition, ticks may be asymptomatically coinfected with different pathogens concurrently and pass these pathogens on to their progeny (transovarian transmission) for maintenance throughout all stages of their development (transstadial transmission). The Borrelia spirochetes, arboviruses, and tick-transmitted viruses all have large animal reservoirs that are an integral part of the ecosystem and cannot be culled or effectively controlled. With the exception of the live-virus yellow fever vaccine and a few others, there are currently no vaccines to prevent mosquito and tick-borne infectious diseases; and disease transmission to humans can only be prevented by arthropod avoidance, insect repellents, and insecticides.

Emerging competent arthropod vectors: mosquitoes and ticks

Mosquitoes are responsible for the transmission of most arthropod-borne infectious diseases worldwide, with malaria responsible for most deaths. Although imported malaria and very few “airport” malaria cases still occur in the United States, marsh drainage projects and mosquito vector control programs have virtually eliminated local malaria transmission in developed nations.

In addition to the encephalitis viruses, autochthonous transmission of other arboviruses, such as dengue and chikingunya viruses, has been reported in Europe and the United States. Most temperate and tropical regions harbor 2 competent mosquito vectors for dengue and chikingunya: the daytime-biting Aedes species, A aegypti and A albopictus (Figure 1).⁷ A albopictus, the Asian tiger mosquito, was introduced into the United States and most of the temperate world from Southeast Asia in the 1960s. A albopictus has now replaced A aegypti as the dominant container-breeding, peridomestic mosquito in many areas, especially in the southeastern United States (Figure 1).^7,10 Although travelers returning from endemic areas in the Caribbean, Africa, India, and Southeast Asia still import most cases of chikingunya and dengue into Europe and the United States, local transmission of both arboviral diseases, for which there are no vaccines or specific treatments, has now occurred in Europe and the United States.^7,8

Figure 1

A, A day-biting female Aedes aegypti mosquito is engorged with blood while feeding on a human. A aegypti is the classical vector of yellow fever, dengue, and chikungunya viruses in the tropics. Recently, Aedes albopictus, seen feeding on a human in B, has proven to be as competent a vector of dengue and chikungunya as A aegypti. A albopictus is now widely distributed worldwide and has been responsible for the autochthonous transmission of dengue in the United States and chikungunya in the United States and Europe. Source: U.S. Centers for Disease Control and Prevention (CDC), Atlanta, GA. CDC Public Health Image Library (PHIL). A: PHIL ID # 9260 B: PHIL ID # 4735. Photograph courtesy of James Gathany, Photographer, CDC.

Today, ticks have emerged as the dominant vectors of infectious disease transmission within the United States and Europe. There are over 14 tick-borne infectious diseases in the United States today, and 3 new tick-transmitted pathogens were recently identified as 1) the heartland virus, transmitted by the lone star tick, Amblyomma americanum; 2) Borrelia miyamotoi transmitted by ixodid ticks; and 3) the 364D rickettsial agent, Rickettsia phillipi (proposed name), transmitted by the Pacific Coast tick, Dermacentor occidentalis (Figure 2).² ^–6

Figure 2

A, A female lone star tick, Amblyomma americanum, is seen “questing” for a host. Note the “lone star” or spot mark located in the center of the dorsal surface. The lone star tick is the vector of 2 newly emerging tick-borne infectious diseases in the southeastern United States, heartland virus disease and southern tick-associated rash illness. B, A female eastern black-legged tick, Ixodes scapularis, is seen “questing” for a host. I scapularis is the most common tick vector of Lyme disease in the eastern United States. Source: US Centers for Disease Control and Prevention (CDC), Atlanta, GA. CDC Public Health Image Library (PHIL). A: PHIL ID # 8683. B: PHIL ID # 1669. Photograph courtesy of James Gathany, Photographer, CDC.

In summary, the proper selection and application of insect repellants are essential for personal protection from arthropod-borne infectious diseases because of the following combination of factors: 1) newly recognized or recently arrived arthropod vectors of infectious diseases, such as ixodid ticks and A albopictus, nurtured by warming climates with longer blood-feeding seasons; 2) international trade and travel transporting tropical pathogens worldwide that thrive in temperate, warming climates, such as WNV, dengue, and chikingunya; and 3) an inability to prevent most arthropod-borne infectious diseases by vaccinations.¹²

Properties of Ideal Insect Repellents

Insect repellants must be effective, safe, and pleasant to apply in children and adults and during pregnancy without damaging skin or clothing. Table 1 presents the most desired characteristics of an ideal insect repellent.¹³

Table 1

Properties of an ideal insect repellent¹³

Effective against broad range or arthropods including fleas, flies, mosquitoes, biting midges (“no-see-ums”), ticks

Can be applied to skin without adverse effects

No damage to clothing (ie, staining, bleaching, thinning)

Can be applied with sunscreen

No odor or has pleasing odor

No oily residues are left on skin

Difficult to remove by washing, wiping, or sweating

No effect on plastics (ie, glasses, watches, upholstery)

Chemically stable

Reasonably priced for broad range of people

Nontoxic

Duration efficacy is adequate

The Efficacy, Safety, and Toxicity of Chemical and Plant-Based Insect Repellents

Insect repellants may be divided into 2 basic chemical classes: 1) the synthetic chemicals, such as DEET, picaridin, and IR3535 (Skin So Soft; Avon Products, Inc., New York, NY); and 2) the plant-derived oils and synthetics, such as oil of lemon eucalyptus, oil of citronella, and permethrin. Table 2 describes the range of insect repellents and insecticides available worldwide as stratified by their active ingredients, formulations, strengths (%), efficacies against arthropods, precautions, and adverse effects.

Table 2

Available insect repellents: formulations, efficacy, safety, and toxicity

Insect repellents (chemical names)	Formulations (strength %)	Efficacy against Anopheline (malaria) mosquitoes	Efficacy against Culicine (arbovirus) mosquitoes	Efficacy against ticks	Efficacy against flies and biting midges (“no-see-ums”)	Toxicity and other adverse effects
DEET (N, N-diethyl-3-methyl-benzamide. Formerly N, N-diethyl-m-toluamide)	Aerosols Lotions Pump sprays Wipes (5–100%)	++	+++	+	++	Potential neurotoxicity if applied under sunscreen. May damage plastic and some synthetic fabric clothing. Safe for cotton, wool, and nylon.
Picaridin (US) and Icaridin (EU) (2-(2-hydroxyethyl)-1-piperidine-carboxylic acid 1-methylpropyl-ester)	Lotions Pump sprays Wipes (7–20%)	++	+++	++	+++ High levels of protection up to 12 hours against Amblyomma americanum	Possible skin irritation. No damage to plastics or clothing.
IR3535 (3-[N-butyl-N-acetyl]-amino-propionic acid ethyl ester)	Aerosols Lotions Pump sprays Wipes (7.5–19.7%)	++	+++ EPA: up to 2 hours protection time for mosquitoes.	++ EPA: up to 3 hours protection time for ticks.	+++	Causes eye irritation. Potential toxicity if ingested or inhaled. May damage plastic and clothing.
Oil of lemon eucalyptus (p-menthane-3, 8-diol)	Pump sprays (10–40%)	+++	+++ EPA: up to 2 hours protection time for mosquitoes.	+++ EPA: up to 2 hours protection time for ticks.	+++	Potential skin irritation in atopic individuals.
Citronella (3, 7-dimethyloct-6-en-1-al) Natural plant oil obtained from Cymbopogon spp. grasses.	Bath oils Candles Lotions (0.5–20%)	+	+	0	0	May damage clothing. Potential eye irritation and skin irritation and allergies.
Permethrin (3-phenoxybenzyl (1RS)-cis, trans-3-(2, 2-dichlorovinyl)-2, 2-dimethyl-cyclo-propane-carboxylate) Pyrethroid derived from dried, crushed flowers of Chrysanthemum spp.	Sprays for clothes, insect nets, sleeping bags, boots (0.5%)	+++	+++	+++	+++	Not useful on skin. Possible skin irritation. Pyrethroid resistance is now developing in mosquitoes. No damage to plastics or clothing.

EPA, Environmental Protection Agency.

Protective efficacy scale: 0, no protection provided; +, minimal level of protection; ++: moderate level of protection; +++, maximal level of protection.

The chemical insect repellents

The first chemical insect repellents included the dialkyl phthalates (dibutyl and dimethyl phthalate), discovered in 1929; indalone, introduced in 1937; and Rutgers 612, introduced in 1939.¹⁴ In 1942, the US Department of Agriculture and the US Army began clinical trials with many chemical compounds to replace the dialkyl phthalates and 612 with less toxic and less oily topical insect repellents effective against a broader variety of insects with a longer duration of action.^13,14 By 1946, N, N-diethyl-3-methylbenzamide (or DEET, previously N, N-diethyl-m-toluamide) was in use by U.S. Armed Forces and later marketed to the public in 1956.^13,14

DEET is available worldwide today in a variety of formulations including aerosols, creams, lotions, sprays, gels, sticks, and wipes (towelettes) at concentrations ranging from 5% to 100%. Most products contain concentrations of 30% to 40% DEET or less, and human studies have now confirmed a plateau insect repellent effect as the concentration of DEET applied topically exceeds 50%.¹⁵ In addition, volunteers who have applied concentrations of 50% to 75% DEET have developed erythema with vesiculobullous skin necrosis and residual scarring.¹⁶ DEET concentrations in the range of 10% to 35% will provide adequate insect bite protection, with concentrations below 30% recommended for children 2 years of age and older.¹⁷ Field testing of topical DEET has demonstrated a longer duration of protection against the Culicine species of mosquitoes that can transmit arboviruses and filarial parasites than against the Anopheline species of mosquitoes that can transmit malaria.¹⁸ In human volunteer trials comparing the duration of tick bite protection provided by insect repellents in simulated forest floor environments populated with ticks (100 host-seeking A americanum nymphs), Carroll and coinvestigators demonstrated that cream-based formulations of 33% DEET and 10% and 20% picaridin provided effective tick bite protection for 12-hour periods.¹⁹

DEET will not damage cotton, wool, or nylon clothing but can damage rayon, spandex, and leather and dissolve plastic and vinyl upholstery.¹⁴ Although DEET does cross the placenta, developmental toxicity has not been described in animals or humans in over 50 years of testing and use by over 30% of the United States population.²⁰ With proper application, the safety record of DEET has proven to be excellent over decades with most cases of toxicity confined to children after overapplications and ingestions.²¹ ^–39

In 2004, Ross and coinvestigators demonstrated increased absorption of DEET applied under sunscreen in a mouse model, which resulted in an FDA recommendation to always apply sunscreen before DEET application.⁴⁰ In addition, the FDA has recommended against using products that combine insect repellents with sunscreens because sunscreens have to be reapplied more often than insect repellants.⁴¹

Between 1956 and 2008, there were 43 confirmed case reports of DEET toxicity: 25 with central nervous system (CNS) involvement, 1 with cardiovascular effects, and 17 with allergic or cutaneous manifestations.²¹ ^–39 The CNS manifestations included lethargy, headache, confusion, disorientation, ataxia, tremors, seizures, and acute encephalopathy with psychosis.²¹ ^–39 Cutaneous manifestations were mostly urticarial reactions and hemorrhagic vesicobullous erosions after topical applications of 50% and stronger preparations.¹⁶ A 61-year-old woman demonstrated orthostatic hypotension and bradycardia after topical application and stabilized within hours of supportive treatment.³⁹ Of the 6 reported deaths attributed to DEET poisoning, 3 followed intentional ingestions, 1 occurred in a child with ornithine transcarbamylase deficiency, and 2 cases occurred in children with convulsive CNS reactions after repeated overapplications.²³ ^–27,37,38

The newest insect repellent, picaridin, or icaridin in Europe (KBR 3023, 2-[2-hydroxyethyl]-1-piperidinecarboxylic acid-1-methylpropyl ester), was developed in Europe in the 1990s, released in the United States about 10 years ago, and offered several immediate advantages over DEET including the lack of a chemical odor, a nonsticky or greasy feel, and lack of any damage to clothing or plastics.¹³ Like DEET, the exact mechanism of action of picaridin is unknown, but its vapor barrier is so noxious to insects’ taste and olfactory senses that it discourages insect contact and biting.¹³ Picaridin is effective against mosquitoes, flies, chiggers (larval Trombiculid mites), and ticks and is available as lotions, sprays, and wipes in strengths of 7% to 20%.¹³ Like DEET, picaridin appears to offer greater protection against Culicine than Anopheline mosquitoes and may offer a longer duration of action and greater protection than DEET against ticks in 20% preparations.¹⁸ In human field trials, Carroll and coinvestigators demonstrated that 10% and 20% concentrations of picaridin provided very high levels of protection for up to 12 hours against lone star ticks (A americanum), the newly recognized insect vectors of heartland virus disease (Figure 2).¹⁹ Human field and animal investigations in Australia and Europe have demonstrated no dermal, solid organ, or reproductive toxicity.^13,14 The manufacturers do not recommend using picaridin in children under 2 years of age.^13,14

IR3535 or ethyl butylacetylaminoproprionate (3-N-butyl-N-acetyl aminoproprionic acid) was initially marketed in the United States as a skin emollient and moisturizer (Avon Skin So Soft) and was quickly adopted for use by hunters because of its greater efficacy against bothersome biting midges, or “no-see-ums,” than DEET. In addition, IR3535 demonstrated greater efficacy against onchocerciasis-transmitting blackflies and leishmaniasis-transmitting sandflies in endemic areas than DEET and was shown to provide a longer duration of protection (mean 10.4 hours) against leishmaniasis-transmitting phlebotamine sand-fly bites than DEET (mean 8.8 hours).⁴² Like DEET and picaridin, IR3535 is more effective at repelling Culicine mosquitoes than Anopheline mosquitoes.⁴³ Although IR3535 has been used in Europe for over 20 years and animal studies have not demonstrated developmental toxicity, there are no specific recommendations for its use or avoidance in children or during pregnancy (FDA Pregnancy Category B).¹⁷

The plant-derived insect repellents

The first effective insect repellents included smoke from burning tar and cooking fires, and a variety of plants and flowers hung in homes or on porches or rubbed on the skin, including chrysanthemum, geranium, and lantana.¹⁴ Many plant oils, such as citronella, clove, geranium, mint, nutmeg, pennyroyal, and soybean would also repel insects for short periods, but their high volatility limited their duration of effectiveness when burned in candles or applied topically.¹⁴

Oil of lemon eucalyptus or p-menthane-3, 8-diol (PMD) is an extract of the leaves of lemon eucalyptus, Corymbia citriodora, or a synthetic version of its major repellent component, PMD.⁴⁴ It is available in pump sprays in concentrations of 10% to 40%. PMD has a mosquito repellant efficacy and duration equal to that of DEET, and, like picaridin, may offer better protection against ticks than DEET.⁴⁴ PMD reduced successful attachment and blood-feeding by 77% against the tick vectors of Lyme disease (Ixodes scapularis, Ixodes pacificus) and Rocky Mountain spotted fever (Dermacentor andersoni) and is also effective against some species of biting midges (Figure 2).⁴⁵ The FDA has recommended that PMD not be used in children under 3 years of age.⁴¹

Citronella (3, 7-dimethyloct-6-en-1-al) is a natural plant oil obtained from several species of Cymbopogon lemongrasses. Citronella is available as a lotion, oil, or solid wax impregnated into candles and flame pots in strengths ranging from 0.5% to 20%. Because of its high volatility, citronella has a short duration of action but can deter nuisance biting by mosquitoes for up to 2 hours.⁴⁴ It is ineffective against flies, fleas, biting midges, and ticks.⁴⁶

Permethrin, first marketed in 1973, is a laboratory-manufactured pyrethroid insect repellant and contact insecticide that is derived from the crushed dried flowers of Chrysanthemum cinerarifolium.¹³ Permethrin is not absorbed topically and requires direct insect contact to be effective.¹³ Its mechanism of action is via initial excitation of the insect’s nervous system by sodium channel blockade followed by acetylcholinesterase inhibition and fatal paralysis.¹³ When applied to clothing, bed nets, tents, and sleeping bags, permethrin and other synthetic pyrethroids (allethrin, alpha-cypermethrin, cyfluthrin, deltamethrin, etofenprox, lambda-cyhalothrin, and metofluthrin) all provide very high-level protection against mosquitoes, flies, biting midges, chiggers, fleas, sandflies, and ticks, especially when combined with topically applied insect repellents.⁴³ Clothing and other products treated with pyrethroids should be retreated after 5 to 70 washings as indicated on the product label to provide continued insect bite protection.⁴⁷ Long-duration, pyrethroid-treated mosquito bed nets are now available that maintain effective insecticide levels for 3 years.¹⁸

Permethrin can kill ticks on contact and provides better tick protection than DEET and picaridin.⁴⁸ Permethrin-impregnated bed nets have provided improved protection of long duration against all Anopheline malaria vectors.¹⁸ Human neurotoxicity with ataxia, hyperactivity, hyperthermia, seizures, and paralysis has been reported after massive ingestions of liquid preparations or inhalations of permethrin-containing sprays.^13,14 Animal studies conducted by the FDA have demonstrated no developmental toxicity from permethrin exposures (FDA Pregnancy Category B).⁴⁹ As would be expected, individuals who are allergic to chrysanthemums must avoid permethrin use.

Although some plant-derived insect repellants are highly effective, such as PMD and permethrin, others only discourage nuisance biting, such as citronella; and others are completely ineffective. Garlic consumption has continued to be recommended as natural insect repellent, but a double-blinded, placebo-controlled trial of garlic consumption to prevent mosquito bites has confirmed garlic’s ineffectiveness as a mosquito repellent.⁵⁰

Comparing chemical and plant-based insect repellents

Laboratory investigations have demonstrated greater efficacy of IR3535 compared with DEET in repelling biting midges and flies and comparative efficacies of PMD and DEET in repelling mosquitoes.⁴² ^–45 Laboratory investigations have also demonstrated greater efficacies of both PMD and picaridin in repelling a broader range of ticks than DEET.^44,45 Field trials comparing the efficacies of DEET and picaridin versus permethrin have continued to demonstrate that permethrin, not DEET, can kill ticks on contact and provides better overall protection against tick bites than both DEET and picaridin.⁴⁸

Although randomized controlled trials do not support recommendations to combine insecticides and repellents, the most effective uses of insect repellents are to layer a topically applied repellent, such as DEET or picaridin, on the skin, with permethrin- or other synthetic pyrethroid-impregnated clothing that act on contact insecticides and provide better and longer lasting protection against malaria-transmitting mosquitoes and ticks. In special cases, where exposures to ticks, biting midges, sandflies, or blackflies are anticipated, topical insect repellents containing IR3535, picaridin, or PMD may offer better protection than topical DEET alone, especially when exposed skin is covered by permethrin-impregnated clothing.⁴¹

According to the CDC and the FDA, insect repellents should not be applied under sunscreens, and all combinations of insect repellents and sunscreens should be avoided.^41,51

Insect repellent use in children and during pregnancy

The FDA has recommended that DEET should not be used in children under 2 years of age, and the American Academy of Pediatrics has recommended a maximum DEET formulation strength of 33% for all children.¹⁷ The FDA has recommended that PMD not be used in children under 3 years of age.¹⁷ Although the manufacturer has recommended avoiding picaridin in children under 2 years of age, most insect repellents, other than DEET, have not been well studied for toxic effects in animal models or human trials.

Toxicological investigations have demonstrated increased systemic absorption of DEET following repeated heavy skin applications with resulting inefficiencies in the hepatic urea cycle’s capacity to detoxify ammonia from dietary protein catabolism.^23,24 The most common disorder of the hepatic urea cycle is ornithine transcarbamylase deficiency (OTD), an X-linked, autosomal recessive, inherited disorder with an incidence of 1 in 80,000 live births.^23,24 Hyperammonemia and metabolic hepatic encephalopathy in infancy are characteristics of OTD.²⁴ Because DEET toxicity with hyperammonemia and encephalopathy has occurred in children after ingestions and overapplications and a fatal case of DEET toxicity was reported in a child with OTD, the use of DEET is relatively contraindicated in individuals with urea cycle disorders, especially OTD.²⁴

With the exception of IR3535 and permethrin, which have been classified by the FDA in Pregnancy Category B (no adverse effects demonstrated in animals), the remaining insect repellents have not been FDA classified by developmental toxicity level (Pregnancy Category N).¹⁷

Area and barrier chemical insect repellents

In addition to topically applied insect repellents and pyrethroid-impregnated clothing, a number of area and barrier methods are used to repel insects, including permethrin-impregnated curtains, screens, and bed nets; insecticide vaporizers; mosquito coils; knockdown insecticide aerosol sprays; and a variety of plant-oil burning candles. Some of these measures are effective; others are not.

Permethrin and other synthetic pyrethroid-treated fabrics have proven highly effective as adjuncts to topical repellents and provide both contact insecticidal and repellent activity. Electric insecticide vaporizers can be set to release pyrethroid insecticides and will inhibit nuisance biting by mosquitoes, but there is no evidence that they will prevent the transmission of arthropod-borne infectious diseases.⁴³ Knockdown insecticide aerosol sprays are designed to kill flying insects indoors, but there is also no evidence to support their use over arthropod avoidance, topical insect repellents, and permethrin-impregnated blinds and curtains.⁴³

Mosquito coils are made from compacted pastes or powders containing pyrethroids and other volatile chemicals, including formaldehyde.^52,53 When lit, the coils will smolder and smoke for hours, discouraging nuisance biting by mosquitoes but contaminating the atmosphere with particulates and volatile chemicals.^53,54 Repeated exposures to mosquito coil smoke may pose significant risk factors for lung disease, including lung cancer.^51,52 Burning a variety of plant oil–based candles may reduce nuisance biting by mosquitoes and flies for a couple of hours, but like aerosol sprays and insecticide vaporizers, there is no evidence to support their use over arthropod avoidance, topical insect repellents, and permethrin-impregnated blinds and curtains.⁴³

Nonchemical measures for the management, control, and prevention of arthropod-borne infectious diseases

To minimize insect bites outdoors, the CDC has recommended that individuals wear long-sleeved shirts, long pants, hats, and boots or covered shoes, not sandals.⁴⁷ In human landing studies with the malaria vector, Anopheles gambiae, Webster and coinvestigators demonstrated that the combination of human skin odors and minute increases above ambient carbon dioxide levels produced synergistic effects that increased mosquito landings.⁵⁴ Although untested in randomized controlled trials, light-colored clothing may limit overheating with concomitant increases in skin odors and exhaled carbon dioxide, both of which are known to attract female mosquitoes to human hosts for blood-feeding.⁵⁴ Individuals should sleep indoors in screened or air-conditioned areas, or under permethrin-impregnated bed-nets in inadequately screened or air-conditioned accommodations.⁴⁷ The Anopheline mosquitoes that transmit malaria typically bite at dawn and dusk, which are prime times to avoid their exposures.⁴⁷ However, the Culicine mosquitoes that transmit dengue, chikingunya, WNV, and yellow fever bite aggressively throughout the day, providing good reasons to apply insect repellents throughout the day when outdoors (Figure 1).⁴⁷

Personal protective measures to prevent tick-borne infectious diseases include wearing long pants tucked into socks, long-sleeved shirts, and light-colored clothing to aide in keeping ticks off of the skin and making them easier to spot on clothing.⁴⁷ Other recommended measures include applying pyrethroid-containing insect repellants to clothing and picaridin, IR3535, or PMD to exposed skin and performing regular whole-body tick checks.⁴⁷

Most patients do not recall painless tick bites, especially bites by diminutive nymphs, and attachment sites may be unseen or hidden by hair. Should tick avoidance measures fail and attached ticks are discovered, ticks should be removed for expert identification, if available, as soon as possible, preferably within 24 hours.⁴⁷ Ticks should be removed with forceps or fine-tipped tweezers gripped close to the point of skin attachment with gentle, steady traction applied to avoid decapitating ticks and leaving imbedded mouthparts with pathogen-filled salivary glands.⁴⁷ Ticks should always be removed with forceps (or tweezers) and not fingers, as crushing ticks can cause them to regurgitate pathogens.⁵⁵ Ticks should be removed in contiguity with their feeding mouthparts, rather than being burnt with spent matches or painted with adhesives, solvents, nail polishes, or nail polish removers.⁴⁷

Conclusions

With few exceptions, there are no vaccines to prevent mosquito and tick-borne infectious diseases, and only arthropod avoidance, insect repellents, and insecticides can prevent disease transmission to humans. The most widely recommended use of insect repellents is to combine topically applied repellents, such as DEET or picaridin, with synthetic pyrethroid-impregnated clothing that acts as contact insecticides and provide better and longer duration protection against mosquitoes and ticks. In special cases, where exposures to ticks, biting midges, sandflies, or blackflies are anticipated, topical insect repellents containing IR3535, picaridin, or PMD offer better protection than DEET alone.

Footnotes

Acknowledgments

The author acknowledges that all support was provided by departmental and institutional sources.

References

Centers for Disease Control and Prevention. Lyme disease. Available at: http://www.cdc.gov/lyme/stats/. Accessed November 17, 2015.

Kosoy

O.I.

Lambert

A.J.

Hawkinson

D.J.

et al. Novel thogotovirus associated with febrile illness and death, United States, 2014. Emerg Infect Dis 2015; 21, 760–764.

Muehlenbachs

Fata

C.R.

Lambert

A.J.

et al. Heartland virus associated death in Tennessee. Clin Infect Dis 2014; 59, 845–850.

Wagemakers

Staarink

P.J.

Sprong

Hovius

J.W.

Borrelia miyamotoi: a widespread tick-borne relapsing fever spirochete. Trends Parasitol 2015; 31, 260–269.

Krause

P.J.

Fish

Narasimhan

Barbour

A.G.

Borrelia miyamotoi infection in nature and in humans. Clin Microbiol Infect 2015; 21, 631–639.

Shapiro

M.R.

Fritz

C.L.

Tait

et al. Rickettsia 364D: a newly recognized cause of eschar-associated illness in California. Clin Infect Dis 2010; 50, 541–548.

Lindsey

N.P.

Prince

H.E.

Kosoy

et al. Chikungunya virus infections among travelers—United States, 2010-2013. Am J Trop Med Hyg 2015; 92, 82–87.

Centers for Disease Control and Prevention. Chikungunya hits US mainland. Available at: http://www.cdc.gov/ncezid/dvbd/. Accessed November 17, 2015..

Deboun

Strickman

Insect repellents and associated personal protection for a reduction in human disease. Med Vet Entomol 2013; 27, 1–9.

10.

Lindsey

N.P.

Lehman

J.A.

Staples

J.E.

Fischer

West Nile virus and other arboviral diseases—United States, 2013. Morb Mort Wkly Rep 2014; 63, 521–526.

11.

Weatherhead

J.E.

Miller

V.E.

Garcia

M.N.

et al. Long-term neurological outcomes in West Nile virus infected-patients: an observational study. Am J Trop Med Hyg 2015; 92, 1006–1012.

12.

Hahn

M.B.

Monaghan

A.J.

Hayden

M.H.

et al. Meteorological conditions associated with increased activity of West Nile virus disease in the United States, 2004-2012. Am J Trop Med Hyg 2015; 92, 1013–1022.

13.

Katz

T.M.

Miller

J.H.

Hebert

A.A.

Insect repellents: historical perspectives and new developments. J Am Acad Dermatol 2008; 58, 865–871.

14.

Brown

Hebert

A.A.

Insect repellents: an overview. J Am Acad of Dermatol 1997; 36, 243–249.

15.

Rutledge

L.C.

Wirtz

P.A.

Buescher

M.D.

Mehr

Z.A.

Mathematical models of the effectiveness and persistence of mosquito repellents. J Am Mosq Control Assoc 1985; 1, 56–61.

16.

McKinlay

J.R.

Ross

E.V.

Barrett

T.L.

Vesicobullous reaction to diethyltoluamide revisited. Cutis 1998; 62, 44.

17.

Abramowicz M, ed. Advice for travelers. Med Lett Drugs Ther. 2015;57:53-58..

18.

Frances

S.P.

Waterston

D.G.E.

Beebe

N.W.

Cooper

R.D.

Field evaluation of repellent formulations containing DEET and picaridin against mosquitoes in Northern Territory, Australia. J Med Entomol 2004; 41, 414–417.

19.

Carroll

J.F.

Benante

J.P.

Klun

J.A.

et al. Twelve hour duration testing of cream formulations of three repellents against Amblyomma americanum. Med Vet Entomol 2008; 22, 144–1451.

20.

McGready

Hamilton

K.A.

Simpson

J.A.

et al. Safety of the insect repellent N, N, diethy-m-toluamide (DEET) in pregnancy. Am J Trop Med Hyg 2001; 65, 285–289.

21.

Briassoulis

Narlioglou

Hatzis

Toxic encephalopathy associated with the use of DEET insect repellents: a case analysis of its toxicity in children. Hum Exp Toxicol 2001; 20, 8–14.

22.

Snyder

J.W.

Poe

R.O.

Stubbins

J.F.

Garrettson

L.K.

Acute manic psychosis following the application of N, N, diethyl-m-toluamide (DEET) in an adult. Clin Toxicol 1986; 24, 429–439.

23.

Heick

H.M.

Petersen

R.G.

Kipe-Scott

Qureshi

I.A.

Insect repellent, N, N, diethyl-m-toluamide, effect on ammonia metabolism. Pediatrics 1988; 82, 373–376.

24.

Heick

H.M.

Shipman

R.T.

Norman

M.G.

James

Reyelike syndrome associated with the use of an insect repellent in a presumed heterozygote for ornithine carbamoyl transferase deficiency. J Pediatr 1980; 97, 471–473.

25.

Leo

R.J.

del Regno

P.A.

Gregory

Clark

K.L.

Insect repellent toxicity associated with psychosis. Psychosomatics 2001; 42, 78–80.

26.

De Garbino

J.P.

Laborde

Toxicity of an insect repellent: N, N-diethyl-m-toluamide. Vet Hum Toxicol 1983; 25, 422–423.

27.

Zadikoff

C.M.

Toxic encephalopathy associated with the use of an insect repellent. J Pediatr 1979; 95, 140–142.

28.

Osimitz

T.G.

Grothaus

R.H.

The present safety assessment of DEET. J Am Mosq Control Assoc 1995; 11, 274–278.

29.

Osimitz

T.G.

Murphy

J.V.

Neurological effects associated with the use of the insect repellent N, N-diethyl-m-toluamide (DEET). J Toxicol Clin Toxicol 1997; 35, 435–441.

30.

Lipscomb

J.W.

Kramer

J.E.

Leikin

J.B.

Seizure following brief exposure to the repellent N, N-diethyl-m-toluamide. Ann Emerg Med 1992; 21, 315–317.

31.

Gryboski

Weinstein

Ordway

N.K.

Toxic encephalopathy apparently related to the use of an insect repellent. N Engl J Med 1961; 264, 289–291.

32.

Roland

E.K.

Jan

J.E.

Rigg

J.M.

Toxic encephalopathy in a child after a brief exposure to insect repellents. Can Med Assoc J 1985; 132, 155–156.

33.

Centers for Disease Control and Prevention

Seizures temporarily associated with the use of DEET insect repellent—New York and Connecticut. MMWR 1989; 38, 678–680.

34.

Edwards

D.L.

Johnson

Insect induced toxic encephalopathy in a child. Clin Pharmacol 1987; 6, 496–498.

35.

Hampers

L.C.

Oker

Leikin

J.B.

Topical use of DEET insect repellent as a cause of severe encephalopathy in a healthy adult male. Acad Emerg Med 1999; 6, 1295–1297.

36.

Veltri

J.C.

Osimitz

T.G.

Bradford

D.C.

Page

B.C.

Retrospective analysis of calls to poison centers resulting from exposure to the insect repellent N, N-diethyl-m-toluamide (DEET) from 1985-1989. J Toxicol Clin Toxicol 1994; 32, 1–16.

37.

Tenenbein

Severe neurotoxic reactions and death following the ingestion of diethyltoluamide-containing insect repellents. J Am Med Assoc 1987; 258, 1509–1511.

38.

Petrucci

Sardini

Severe neurotoxic reaction associated oral ingestion of low-dose diethyltoluamide-containing insect repellent in a child. Pediatr Emerg Care 2000; 16, 341–342.

39.

Clem

J.R.

Havemann

D.F.

Raebel

M.A.

Insect repellent (N, N-diethyl-m-toluamide) cardiovascular toxicity in an adult. Ann Pharmacother 1993; 27, 289–293.

40.

Ross

E.A.

Savage

K.A.

Utley

L.J.

Tebbett

I.R.

Insect repellent interactions: sunscreens enhance DEET (N, N-diethyl-m-toluamide) absorption. Drug Metab Dispos 2004; 32, 783–785.

41.

Hexsel

C.L.

Bangert

S.D.

Hebert

A.A.

Lim

H.W.

Current sunscreen issues: 2007 Food and Drug Administration sunscreen labelling recommendations and combination sunscreen/insect repellent products. J Am Acad Derm 2008; 59, 316–323.

42.

Naucke

T.J.

Lorentz

Grünewald

H.W.

Laboratory testing of the insect repellents IR3535 and DEET against Phlebotomus mascitti and P. duboscqi (Diptera: Psychodidae). Int J Med Microbiol 2006; 40S, 230–232.

43.

Goodyer

L.I.

Croft

A.M.

Frances

S.P.

et al. Expert review of the evidence base for arthropod bite avoidance. J Travel Med 2010; 17, 182–192.

44.

Carroll

S.P.

PMD

Loye J.

A registered botanical mosquito repellent with DEET-like efficacy. J Am Mosq Cont Assoc 2006; 22, 507–514.

45.

Trigg

J.K.

Hill

Laboratory evaluation of a eucalyptus-based repellent against four biting arthropods. Phytother Res 1996 10313–10316.

46.

Tawatsin

Wratten

S.D.

Scott

R.R.

Thavara

Techadamrongsin

Repellency of volatile oils from plants against three mosquito vectors. J Vect Ecol 2001; 26, 76–82.

47.

Nasci RS, Wirtz RA, Brogdon WG. Chapter 2. The pre-travel consultation. Protection against mosquitoes, ticks, and other insects and arthropods. US Centers for Disease Control and Prevention. Available at: http://wwwnc.cdc.gov/travel/yellowbook/2016/the-pre-travel-consultation/protection-against-mosquitoes-ticks-other-arthropods. Accessed November 17, 2015..

48.

Solberg

V.B.

Klein

T.A.

McPherson

K.R.

Bradford

B.A.

Burge

J.R.

Wirtz

R.A.

Field evaluation of DEET and a piperidine repellent (A13-37220) against Amblyomma americanum (Acari: Ixodidae). J Med Entomol 1995; 32, 870–875.

49.

US Environmental Protection Agency. Prevention, pesticides and toxic substances (7508P). Permethrin Facts. EPA 738-F-09-001; August 2009:1–11..

50.

Rajan

T.V.

Hein

Porte

Wikel

A double-blinded, placebo-controlled trial of garlic as a mosquito repellent; a preliminary study. Med Vet Entomol 2005; 19, 84–89.

51.

US Centers for Disease Control and Prevention. Insect repellent use and safety. Available at: www.cdc.gov/westnile/FAQ/repellent.html. Accessed November 17, 2015..

52.

Chen

S.C.

Wong

R.H.

Shiu

L.J.

Chiou

M.C.

Lee

Exposure to mosquito coil smoke may be a risk factor for lung cancer in Taiwan. J Epidemiol 2008; 18, 19–25.

53.

Liu

Zhang

Hashim

J.H.

Jalaludin

Hashim

Goldstein

B.D.

Mosquito coil emissions and health implications. Environ Health Perspect 2003; 111, 1454–1460.

54.

Webster

Lacey

E.S.

Cardé

R.T.

Waiting with baited breath: opportunistic orientation to human odor in the malaria mosquito, Anopheles gambiae, is modulated by minute changes in carbon dioxide concentration. J Chem Ecol 2015; 41, 59–66.

55.

Parola

Raoult

Ticks and tickborne bacterial diseases: an emerging infectious threat. Clin Infect Dis 2001; 32, 897–928.

Chemical and Plant-Based Insect Repellents: Efficacy,Safety,and Toxicity

Abstract

Keywords

Introduction

Methods

Results

Definitions

Why Use Insect Repellents?

The Threats from Emerging and Imported Arthropod-Borne Infectious Diseases

Emerging competent arthropod vectors: mosquitoes and ticks

Properties of Ideal Insect Repellents

The Efficacy, Safety, and Toxicity of Chemical and Plant-Based Insect Repellents

The chemical insect repellents

The plant-derived insect repellents

Comparing chemical and plant-based insect repellents

Insect repellent use in children and during pregnancy

Area and barrier chemical insect repellents

Nonchemical measures for the management, control, and prevention of arthropod-borne infectious diseases

Conclusions

Footnotes

Acknowledgments

References