Free accessResearch articleFirst published online 2013-09
Anti-Inflammatory Entrapment in Polycaprolactone/Silica Hybrid Material Prepared by Sol-Gel Route,Characterization,Bioactivity and in Vitro Release Behavior
A novel organic/inorganic hybrid material, based on poly(ε-caprolactone) (PCL) and silica (SiO2), were synthesized by the sol-gel method. An anti-inflammatory agent (indomethacin) was incorporated into the hybrid material to verify its local controlled drug delivery system.
Methods
The structure of the interpenetrating network was investigated by Fourier transform infrared spectroscopy. The morphology of the materials was studied by scanning electron microscopy. The structure of a molecular level dispersion was disclosed by atomic force microscopy.
The bioactivity of the synthesized hybrid materials was revealed by the formation of a layer of hydroxyapatite on the surface of samples soaked in a simulated body fluid (sBF). Release kinetics in SBF were subsequently investigated. The amount of drug released was detected by UV-VIS spectroscopy.
Results
Pure anti-inflammatory agent exhibited linear release with time; in contrast, sol-gel silica entrapped drugs were released with a logarithmic time dependence starting with an initial burst effect followed by a gradual decrease.
Conclusions
SiO2/PCL (3, 6, 9 and 12 %wt) materials, prepared via sol-gel process, are organic/inorganic hybrid and bioactive materials. All the materials showed a good release and therefore could be used as drug delivery system.
Vallet-RegíM.Ceramics for medical applications.Journal of the Chemical Society, Dalton Transactions2001; 2: 97–108.
2.
Vallet-RegíM., ArcosD.Nanostructured hybrid materials for bone tissue regeneration.Curr Nanosci2006; 2: 179–89.
3.
Vallet-RegíM.Ordered mesoporous materials in the context of drug delivery systems and bone tissue engineering.Chemistry - A European Journal2006; 12: 5934–43.
4.
Vallet-RegíM.Bone repair and regeneration: possibilities.Materwiss Werksttech2006; 37: 478–84.
FitzgeraldJ.J., LandryC.J.T., PochanJ.M.Dynamic studies of the molecular relaxations and interactions in microcomposites prepared by in-situ polymerization of silicon alkoxides.Macromolecules1992; 25: 3715–22.
KokuboT., KimH-M, KawashitaM., NakamuraT.Process of calcification on artificial materials. Z.Kardiol.2001; 90: iii/86–iii/91.
27.
OhtsukiC., KokuboT., YamamuroT.Mechanism of apatite formation on CaO • SiO2 • P2O5 glasses in a simulated body fluid.J Non-Crys Solids1992; 143: 84–92.
28.
HutmacherD.W., SchantzT., ZeinI., Woei NgK., Hin TeohS., Cheng TanK.Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling.J Biomed Mater Res2001; 55: 203–16.
29.
KhorH.L., NgK.W., SchantzJ.T.Poly(∊-caprolactone) films as a potential substrate for tissue engineering an epidermal equivalent.Mat Sci Eng C2002; 20: 71–5.
30.
AllenC., HanJ., YuY., MaysingerD., EisenbergA.Polycaprolactone-b-poly(ethylene oxide) copolymer micelles as a delivery vehicle for dihydrotestosterone.J Control Release2000; 63: 275–86.
31.
ChenB., EvansJ.R.G.Poly(∊-caprolactone)-clay nanocomposites: structure and mechanical properties.Macromolecules2005; 39: 747–54.
32.
MessersmithP.B., GiannelisE.P.Polymer-layered silicate nanocomposites: in situ intercalative polymerization of ∊-caprolactone in layered silicates.Chem Mater1993; 5: 1064–6.
33.
NieK., PangW., WangY., LuF., ZhuQ.Effects of specific bonding interactions in poly(∊-caprolactone)/silica hybrid materials on optical transparency and melting behavior.Mater Lett2005; 59: 1325–8.
34.
WuC-S. In situ polymerization of titanium isopropoxide in polycaprolactone: Properties and characterization of the hybrid nanocomposites.J Appl Polym Sci2004; 92: 1749–57.
35.
CatauroM., RaucciM.G., De GaetanoF., MarottaA.Sol-gel synthesis, characterization and bioactivity of polycaprolactone/SiO2 hybrid material.J Mater Sci2003; 38: 3097–102.
36.
CatauroM., RaucciM.G., de GaetanoF., BuriA., MarottaA., AmbrosioL.Sol–gel synthesis, structure and bioactivity of polycaprolactone/CaO • SiO2 hybrid material.J Mater Sci Mater Med2004; 15: 991–5.
37.
De GaetanoF., AmbrosioL., RaucciM.G., MarottaA., CatauroM.Sol-gel processing of drug delivery materials and release kinetics.J Mater Sci Mater Med2005; 16: 261–5.
38.
CatauroM., RaucciM.G., MarcoDd, AmbrosioL.Release kinetics of ampicillin, characterization and bioactivity of TiO2/PCL hybrid materials synthesized by sol-gel processing.J Biomed Mater Res A2006; 77A: 340–50.
39.
CatauroM., RaucciM., AusanioG.Sol-gel processing of drug delivery zirconia/polycaprolactone hybrid materials.J Mater Sci Mater Med2008; 19: 531–40.
40.
De SantisR., CatauroM., Di SilvioL.Effects of polymer amount and processing conditions on the in vitro behaviour of hybrid titanium dioxide/polycaprolactone composites.Biomaterials2007; 28: 2801–09.
41.
TeoliD., ParisiL., RealdonN., GuglielmiM., RosatoA., MorpurgoM.Wet sol-gel derived silica for controlled release of proteins.J Control Release2006; 116: 295–303.
42.
HenchL.L., ClarkD.E.Physical chemistry of glass surfaces.J Non Cryst Solids1978; 28: 83–105.
43.
PaulA eds. Chemistry of glasses.New York: Chapman and Hall1990; 184.
44.
WangD.S., PantanoC.G.Surface chemistry of multicomponent silicate gels.J Non Cryst Solids1992; 147–148: 115–22.
45.
SanchezC., LivageJ., HenryM., BabonneauF.Chemical modification of alkoxide precursors.J Non Cryst Solids1988; 100: 65–76.
