Strengthened by the analysis of genetic engineering approaches and strategies related to transgenic animal models and of the most cutting-edge imaging techniques, this article will be centered on the impact that such applications have and will have in research and, as a consequence, in the clinical area.
In order to show how identical techniques can be applied in different contexts for the study of different organs and tissues, in this issue the applications of these technologies in the anatomical and functional studies of distinct organs such as the kidney and the central nervous system will be analyzed. In particular, concrete examples about the kidney and regenerative nephrology will be exploited to show how different techniques can lead to similar, or diametrically opposite data, depending on the approach used to reach the specific research or diagnostic goal.
LombardiD., AlteriniT.New techniques of transgenesis and imaging: applications in Neurology and Nephrology. Part I.Giornale di Tecniche Nefrologiche e Dialitiche2014; 26(2): 173–81.
2.
LombardiD., LasagniL.Transgenic Strategies to Study Podocyte Loss and Regeneration.Stem Cells Int2015; 2015: 678347.
3.
AlexanderA.L.Diffusion tensor imaging of the brain.Neurotherapeutics2007; 4(3): 316–29.
4.
DenkW., HorstmannH.Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure.PLoS Biol2004; 2(11): e329.
5.
StarborgT.Using transmission electron microscopy and 3View to determine collagen fibril size and three-dimensional organization.Nat Protoc2013; 8(7): 1433–48.
6.
OheimM.Two-photon microscopy in brain tissue: parameters influencing the imaging depth.J Neurosci Methods2001; 111(1): 29–37.
HacklM.J.Tracking the fate of glomerular epithelial cells in vivo using serial multiphoton imaging in new mouse models with fluorescent lineage tags.Nat Med2013; 19(12): 1661–6.
WickmanL.Urine podocyte mRNAs, proteinuria, and progression in human glomerular diseases.J Am Soc Nephrol2013; 24(12): 2081–95.
22.
SagrinatiC.Isolation and characterization of multipotent progenitor cells from the Bowman's capsule of adult human kidneys.J Am Soc Nephrol2006; 17(9): 2443–56.
23.
LasagniL.Podocyte Regeneration Driven by Renal Progenitors Determines Glomerular Disease Remission and Can Be Pharmacologically Enhanced.Stem Cell Reports2015; 5(2): 248–63.
24.
RankinE.B., TomaszewskiJ.E., HaaseV.H.Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor.Cancer Res2006; 66(5): 2576–83.
25.
LasagniL., RomagnaniP.Glomerular epithelial stem cells: the good, the bad, and the ugly.J Am Soc Nephrol2010; 21(10): 1612–9.
26.
SchiesslI.M., BardehleS., CastropH.Superficial nephrons in BALB/c and C57BL/6 mice facilitate in vivo multiphoton microscopy of the kidney.PLoS One2013; 8(1): e52499.
27.
DeviS.Multiphoton imaging reveals a new leukocyte recruitment paradigm in the glomerulus.Nat Med2013; 19(1): 07–12.
28.
Peti-PeterdiJ., Multiphoton imaging of renal regulatory mechanisms.Physiology (Bethesda)2009; 24: 88–96.
29.
Peti-PeterdiJ., SiposA.A high-powered view of the filtration barrier.J Am Soc Nephrol2010; 21(11): 1835–41.
30.
BurfordJ.L.Intravital imaging of podocyte calcium in glomerular injury and disease.J Clin Invest2014; 124(5): 2050–8.
31.
SalmonA.H.Loss of the endothelial glycocalyx links albuminuria and vascular dysfunction.J Am Soc Nephrol2012; 23(8): 1339–50.
32.
NakanoD.Multiphoton imaging of the glomerular permeability of angiotensinogen.J Am Soc Nephrol2012; 23(11): 1847–56.
33.
ChenT.W.Ultrasensitive fluorescent proteins for imaging neuronal activity.Nature2013; 499(7458): 295–300.
34.
AhrensM.B.Whole-brain functional imaging at cellular resolution using light-sheet microscopy.Nat Methods2013; 10(5): 413–20.
35.
LombardiD., BecherucciF., RomagnaniP.How much can the tubule regenerate and who does it? An open question.Nephrol Dial Transplant2015.
36.
PavenstadtH., KrizW., KretzlerM.Cell biology of the glomerular podocyte.Physiol Rev2003; 83(1): 253–307.
37.
RemuzziG., BenigniA., RemuzziA.Mechanisms of progression and regression of renal lesions of chronic nephropathies and diabetes.J Clin Invest2006; 116(2): 288–96.
38.
LasagniL.Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.Stem Cells2010; 28(9): 1674–85.
39.
RonconiE.Regeneration of glomerular podocytes by human renal progenitors.J Am Soc Nephrol2009; 20(2): 322–32.
40.
AppelD.Recruitment of podocytes from glomerular parietal epithelial cells.J Am Soc Nephrol2009; 20(2): 333–43.
41.
RomagnaniP., RinkevichY., DekelB.The use of lineage tracing to study kidney injury and regeneration.Nat Rev Nephrol2015; 11(7): 420–31.
42.
SchaunM.I.Cell Therapy in Ischemic Heart Disease: Interventions That Modulate Cardiac Regeneration.Stem Cells Int2016; 2016: 2171035.
43.
SmeriglioP.Phosphotyrosine phosphatase inhibitor bisperoxovanadium endows myogenic cells with enhanced muscle stem cell functions via epigenetic modulation of Sca-1 and Pw1 promoters.FASEB J2015.
44.
ShiJ., LongoF.M., MassaS.M.A small molecule p75(NTR) ligand protects neurogenesis after traumatic brain injury.Stem Cells2013; 31(11): 2561–74.
45.
BarkerN.Lgr5(+ve) stem/progenitor cells contribute to nephron formation during kidney development.Cell Rep2012; 2(3): 540–52.
46.
BarkerN.Identification of stem cells in small intestine and colon by marker gene Lgr5.Nature2007; 449(7165): 1003–7.
47.
BarkerN.Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro.Cell Stem Cell2010; 6(1): 25–36.
48.
HuchM.In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration.Nature2013. 494(7436): 247–50.
LiuD.Leucine-rich repeat-containing G-protein-coupled Receptor 5 marks short-term hematopoietic stem and progenitor cells during mouse embryonic development.J Biol Chem2014; 289(34): 23809–16.
51.
DresslerG.R.Epigenetics, development, and the kidney.J Am Soc Nephrol2008; 19(11): 2060–7.
52.
DresslerG.R.The specification and maintenance of renal cell types by epigenetic factors.Organogenesis2009; 5(2): 73–82.
53.
SallustioF.TLR2 plays a role in the activation of human resident renal stem/progenitor cells.FASEB J2010; 24(2): 514–25.
54.
LoverreA.Increase of proliferating renal progenitor cells in acute tubular necrosis underlying delayed graft function.Transplantation2008; 85(8): 1112–9.
55.
PeiredA.Proteinuria impairs podocyte regeneration by sequestering retinoic acid.J Am Soc Nephrol2013; 24(11): 1756–68.
56.
WachsteinM.Histochemical staining reactions of the normally functioning and abnormal kidney.J Histochem Cytochem1955; 3(4): 246–70.
57.
CooperW.G., RegerJ.F., Van BreemenV.L.Observations on the basement membranes in rat kidney.J Biophys Biochem Cytol1956; 2(4 Suppl.): 283–6.
58.
PenneyD.P.Analysis and testing of biological stains–the Biological Stain Commission Procedures.Biotech Histochem2002; 77(5-6): 237–75.
59.
BonsibS.M., ReznicekM.J.Renal biopsy frozen section: a fluorescent study of hematoxylin and eosin-stained sections.Mod Pathol1990; 3(2): 204–10.
60.
Sims-LucasS.Analysis of 3D branching pattern: hematoxylin and eosin method.Methods Mol Biol2012; 886: 73–86.
61.
CohenA.H.Masson's trichrome stain in the evaluation of renal biopsies. An appraisal.Am J Clin Pathol1976; 65(5): 631–43.
62.
FarrisA.B.Morphometric and visual evaluation of fibrosis in renal biopsies.J Am Soc Nephrol2011; 22(1): 176–86.
63.
HopwoodD.Fixatives and fixation: a review.Histochem J1969; 1(4): 323–60.
64.
MolneJ., BreimerM.E., SvalanderC.T.Immunoperoxidase versus immunofluorescence in the assessment of human renal biopsies.Am J Kidney Dis2005; 45(4): 674–83.
65.
SchenaF.P., GesualdoL.Renal biopsy–beyond histology and immunofluorescence.Nephrol Dial Transplant1994; 9(11): 1541–4.
66.
PepperkokR., EllenbergJ.High-throughput fluorescence microscopy for systems biology.Nat Rev Mol Cell Biol2006; 7(9): 690–6.
67.
LeblondF.Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applications.J Photochem Photobiol B2010; 98(1): 77–94.
68.
HaasM.A reevaluation of routine electron microscopy in the examination of native renal biopsies.J Am Soc Nephrol1997; 8(1): 70–6.
