TO investigate the distribution of transforming growth factor-β (TGF-β) and cells derived from the retinal pigment epithelium (RPE) as a major source for TGF-β in subretinal strands (SRSs), originating from patients with proliferative vitreoretinopathy (PVR) and proliferative diabetic vitreoretinopathy (PDVR).
Methods
Nine SRSs (PVR: n = 6 and PDVR: n = 3) were obtained during pars plana vitrectomy. Specimens were processed for immunohistochemical analysis in paraffin wax or studied by transmission electron microscopy.
Results
Staining for TGF-β was consistent in all specimens. The content of RPE-derived cells identified by anticytokeratin staining revealed considerable differences, ranging from <10 to >90%.
Conclusions
The widespread immunoreactivity for TGF-β in SRSs suggests a significant role of this group of cytokines in the pathologic course of PVR.
SchwartzD., de la CruzZ., GreenW.R., MichelsR.G.Proliferative vitreoretinopathy: ultrastructural study of 20 retroretinal membranes removed by vitreous surgery. Retina1988; 8: 275–81.
7.
HiscottP., MorinoI., AlexanderR., GriersonI., GregorZ.Cellular components of subretinal membranes in proliferative vitreoretinopathy. Eye1989; 3: 606–10.
8.
Garcia-ArumiJ., CorcosteguiB., TalladaN.Subretinal membranes in proliferative vitreoretinopathy. Retina1992; 12: S55–9.
9.
LaquaH.Collagen formation by periretinal cellular membranes. Dev Ophthalmol1981; 2: 396–406.
10.
HiscottP., WallerH.A., GriersonI.Fibronectin synthesis in subretinal membranes of proliferative vitreoretinopathy. Br J Ophthalmol1992; 76: 486–90.
11.
ChenY-S, HackettS.F., SchoenfeldC-L, VinoresM.A., VinoresS.A., CampochiaroP.A.Localisation of vascular endothelial growth factor and its receptors to cells of vascular and avascular epiretinal membranes. Br J Ophthalmol1997; 81: 919–26.
12.
ChenY.S., HackettS.F., SchoenfeldC.L., VinoresM.A., VinoresS.A., CampochiaroP.A.Localisation of vascular endothelial growth factor and its receptors to cells of vascular and avascular epiretinal membranes. Br J Ophthalmol1997; 81: 919–26.
13.
ArmstrongD., AugustinA.J., SpenglerR.Detection of vascular endothelial growth factor and tumor necrosis factor alpha in epiretinal membranes of proliferative diabetic retinopathy, proliferative vitreoretinopathy and macular pucker. Ophthalmologica1998; 212: 410–4.
14.
Bochaton-PiallatM.L., KapetaniosA.D., DonatiG., RedardM., GabbianiG., PournarasC.J.TGF-beta1, TGF-beta receptor II and ED-A fibronectin expression in myofibroblast of vitreoretinopathy. Invest Ophthalmol Vis Sci2000; 41: 2336–42.
15.
BaudouinC., Fredj-ReygrobelletD., BrignoleF., NegreF., LapalusP.H., GastaudP.Growth factors in vitreous and subretinal fluid cells from patients with proliferative vitreoretinopathy. Ophthalmic Res1993; 25: 52–9.
16.
RaymondM.C., ThompsonJ.T.RPE-mediated collagen gel contraction. Inhibition by colchicine and stimulation by TGF-beta. Invest Ophthalmol Vis Sci1990; 31: 1079–86.
17.
ChoudhuryP., ChenW., HuntR.C.Production of platelet-derived growth factor by lnterleukin-1beta and transforming growth factor-beta stimulated retinal pigment epithelial cells leads to contraction of collagen gels. Invest Ophthalmol Vis Sci1997; 38: 824–33.
18.
MachemerR., AabergT.M., FreemanH.M., IrvineA.R., LeanJ.S., MichelsR.M.An updated classification of retinal detachment with proliferative vitreoretinopathy. Am J Ophthalmol1991; 112: 159–65.
19.
WinklerJ., HagelsteinS., RohdeM., LaquaH.Cellular and cytoskeletal dynamics within organ cultures of porcine neuroretina. Exp Eye Res2002; 74: 777–88.
20.
BartekJ., VojtesekB., StaskovaZ.A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol1991; 164: 215–24.
21.
GaudricA., Glacet-BernardA., ClémentG., FalquerhoL., BarritaultD., CoscasG.Transforming growth factor beta in the vitreous of patients with epiretinal proliferation. Ophthalmologe1990; 4: 51–2.
22.
HiraseK., SugiyamaT., IkedaT.Transforming growth factor beta (2) increases in subretinal fluid in rhegmatogenous retinal detachment with subretinal strands. Ophthalmologica2005; 219: 222–5.
23.
PfefferB.A., FlandersK.C., GuerinC.J., DanielpourD., AndersonD.H.Transforming growth factor beta 2 is the predominant isoform in the neural retina, retinal pigment epithelium-choroid and vitreous of the monkey eye. Exp Eye Res1994; 59: 323–33.
24.
TaniharaH., YoshidaM., MatsumotoM., YoshimuraN.Identification of transforming growth factor- expressed in cultured human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci1993; 34: 413–9.
25.
GuerinC.J., HuL., ScicliG., ScicliA.G.Transforming growth factor beta in experimentally detached retina and periretinal membranes. Exp Eye Res2001; 73: 753–64.
26.
GuidryC., KingJ.L., MasonJ.O.Fibrocontractive Muller cell phenotypes in proliferative diabetic retinopathy. Invest Ophthalmol Vis Sci2008; 50: 1929–39.
27.
HardwickC., FeistR., MorrisR.Tractional force generation by porcine Müller cells. Invest Ophthalmol Vis Sci1997; 38: 2053–63.
