Abstract
The aim of this study was to develop an assay method for determination of down stream gene expression in endothelial cells from human decidual tissue preserved in an RNA-conserving buffer. Human decidual tissue was collected and preserved in RNAlater. The tissue samples were dissected and passed through a mechanical tissue disaggregator, incubated with CD31-coated paramagnetic beads and the attached cells were extracted. RNA isolated from the enriched cells proved to be conserved with good quality and a genome RNA-microarray was successfully performed. The method provides an alternative for studying in vivo gene expression in endothelial cells from human decidual tissue.
Get full access to this article
View all access options for this article.