The Moroccan diet incorporates olive oil as the primary source of fat and may reduce cancer risk. However, different olive oil varieties often have varying levels of anti-cancer polyphenols and thus have unique biologic effects.
OBJECTIVE:
The anti-cancer activity of five varieties of extra virgin Moroccan-cultivated olive oil on human cervical cancer cells was assessed in vitro.
METHODS:
The presence of phenolic compounds in five olive oil varieties cultivated in Morocco was analyzed using HPLC. Human cervical cancer cell lines (HeLa, SKG-II, and HCS-2) were incubated with the olive oils and cell viability was measured by MTT assay, reactive oxygen species were measured using the CellRox assay, and gene expression was measured by RT-PCR.
RESULTS:
Each of the five Moroccan-cultivated olive oil varieties had a unique composition of phenolic compounds. Incubation with the olive oils reduced cell viability and reactive oxygen species in human cervical cancer cells. The expression of genes involved in cervical cancer carcinogenesis and cell cycle were also altered. All five olive oil varieties decreased expression of E6, E7, p16, p63, and NRP2 and increased expression of IVL and miR 331-3p.
CONCLUSIONS:
Use of Moroccan-cultivated olive oils could be a promising anti-cancer agent for cervical cancer.
El HilaliH, El HilaliF, SamihM, LaziriF. Cancer incidence in Northern Central Moroccan patients. Journal of Medical and Surgical Research. 2017;4:403–9.
2.
EstruchR, RosE, Salas-SalvadóJ, CovasMI, CorellaD, ArosF, et al.Primary prevention of cardiovascular disease with a Mediterranean diet. N Engl J Med. 2018;378:2441–2.
3.
BaroneM, NotarnicolaM, CarusoMG, ScavoMP, ViggianiMT, TutinoV, et al.Olive oil and omega-3 polyunsaturated fatty acids suppress intestinal polyp growth by modulating the apoptotic process in ApcMin/+mice. Carcinogenesis. 2014;35:1613–9.
4.
BossA, BishopKS, MarlowG, BarnettMPG, FergusonLR. Evidence to support the anti-cancer effect of olive leaf extract and future directions. Nutrients. 2016;8:513.
FabianiR. Anti-cancer properties of olive oil secoiridoid phenols: a systematic review of in vivo studies. Food Funct2016;12:4145–59.
7.
WangSJ, ZhengCJ, PengC, ZhangH, JiangYP, HanT, et al.Plants and cervical cancer: an overview. Expert Opin Investig Drugs. 2013;22:1133–56.
8.
AhmedKL. The cytotoxic effect of olive oil, nigella sativa and their combination on Hela cancer cell line (in vitro). Journal for Veterinary Medical Sciences. 2015;6:87–97.
9.
AhmadR, AhmadN, NaqviAA, ShehzadA, Safer Al-GhamdiM. Role of traditional Islamic and Arabic plants in cancer therapy. Journal of Traditional and Complementary Medicine. 2017;7:195–204.
10.
BattinoM, Forbes-HernandezTY, GasparriniM, AfrinS, CianciosiD, ZhangJ, et al.Relevance of functional foods in the Mediterranean diet: the role of olive oil, berries and honey in the prevention of cancer and cardiovascular disease. Crit Rev Food Sci Nutr. 2019;59:893–920.
11.
SimonL, Saez LancellottiTE, CorteseL, VeisagaM, ChantarasinlapinP, BarbieriA, et al.Olive oil addition to the high-fat diet reduces methylglyoxal (MG-H1) levels increased in hypercholesterolemic rabbits. Mediterranean Journal of Nutrition and Metabolism. 2019;12:13–21.
12.
BenlarbiF, StokerP, YousfiM. Investigation of antioxidant and antihemolytic activities of Algerian defatted olive fruits (olea europaea L.) at two ripening stages. Mediterranean Journal of Nutrition and Metabolism. 2018;11:217–33.
13.
Ramirez-TortosaC, Varela-LopezA, Navarro-HortalMD, Ramos-PleguezelosFM, Marquez-LoboB, Ramirez-TortosaM, et al. Longevity and cause of death in maleWistar rats fed lifelong diets based on virgin olive oil, sunflower oil, or fish oil. The Journals of Gerontology. 2019;glz091.
14.
El-AzemN, Pulido-MoranM, Ramirez-TortosaCL, QuilesJL, CaraFE, Sanchez-RoviraP, et al.Modulation by hydroxytyrosol of oxidative stress and antitumor activities of paclitaxel in breast cancer. Eur J Nutr. 2019;58:1203–11.
15.
Robles-AlmazanM, Pulido-MoranM, Moreno-FernandezJ, Ramirez-TortosaC, Rodriguez-GarciaC, QuilesJL, et al.Hydroxytyrosol: Bioavailability, toxicity, and clinical applications. Food Res Int. 2018;105:654–67.
16.
Varela-LopezA, Perez-LopezMP, Ramirez-TortosaCL, BattinoM, Granados-PrincipalS, Ramirez-TortosaMDC, et al.Gene pathways associated with mitochondrial function, oxidative stress and telomere length are differentially expressed in the liver of rats fed lifelong on virgin olive, sunflower or fish oils. J Nutr Biochem. 2018;52:36–44.
17.
LeGendreO, BreslinPAS, FosterDA. Oleocanthal rapidly and selectively induces cancer cell death via lysosomal membrane permeabilization. Journal Molecular & Cellular Oncology. 2015;2:4.
18.
YaoJ, WuJ, YangX, YangJ, ZhangY, DuL. Oleuropein induced apoptosis in HeLa cells via a mitochondrial apoptotic cascade associated with activation of the c-Jun NH2- terminal kinase. J Pharmacol Sci. 2014;125:300–11.
19.
Reboredo-RodríguezP, González-BarreiroC, Cancho-GrandeB, Forbes-HernandezTY, GasparriniM, AfrinS, et al.Characterization of phenolic extracts from Brava extra virgin olive oils and their cytotoxic effects on MCF-7 breast cancer cells. Food Chem Toxicol. 2013;119:73–85.
20.
Erol-DayiÖ, ArdaN, ErdemG. Protective effects of olive oil phenolics and gallic acid on hydrogen peroxide-induced apoptosis. Eur J Nutr. 2012;51:955–60.
21.
Erol-DayiÖ, ArdaN, ErdemG. Phenols of virgin olive oil protects nuclear DNA against oxidative damage in HeLa cells. Food Chem Toxicol. 2012;50:3475–9.
22.
FabianiR, RosignoliP, De BartolomeoA, FuccelliR, ServiliM, MontedoroGF, et al.Oxidative DNA damage is prevented by extracts of olive oil, hydroxytyrosol, and other olive phenolic compounds in human blood mononuclear cells and HL60 cells. J Nutr. 2013;138:1411–6.
23.
BrenesM, GarciaA, RiosJJ, GarciaP, GarridoA. Development of methods for the classification of EVOOs according to their geographical origin. Int J Food Sci Technol. 2002;37:615–25.
24.
RomeroCN, BrenesM, YousfiK, GarciaP, GarciaA, GarridoA. Effect of cultivar and processing method on the contents of polyphenols in table olives. J Agric Food Chem. 2004;52:479–84.
25.
García-VillalbaR, PacchiarottaT, Carrasco-PancorboA, Segura-CarreteroA, Fernandez-GutierrezA, DeelderetAM, et al.Gas chromatography-atmospheric pressure chemical ionization-time of flight mass spectrometry for profiling of phenolic compounds in extra virgin olive oil. Journal of Chromatography. 2011;1218:959–71.
26.
ChenaX, JiangJ, ShenbH, HubZ. Genetic susceptibility of cervical cancer. Journal of Biomedical Research.. 2011;25:155–64.
27.
zur HausenH. Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer. 2002;2:342–50.
28.
EscrichE, SolanasM, MoralR. Olive oil and other dietary lipids in breast cancer. Cancer Treat Res. 2014;159:289–309.
29.
KimuraE, EnnsRE, ThiebautF, HowellSB. Regulation of HSP60 mRNA expression in a human ovarian carcinoma cell line. Cancer Chemother Pharmacol. 1993;32:279–85.
30.
Ortiz-OrtizJ, Alarcón-RomeroLC, Jiménez-LópezMA, Garzon-BarrientosVH, Calleja-MaciasI, Barrera-SaldanaHA, et al.Association of human papillomavirus 16 E6 variants with cervical carcinoma and precursor lesions in women from Southern Mexico. Virol J. 2015;12:29.
31.
LeouifoudiI, MbarkiM, TilaouiM, AmechrouqA, RakibEM, MouseHA, et al.Study of the in vitro anticancer activity of Moroccan phenolic olive cake extracts. Journal of Pharmacognosy and Phytochemistry. 2014;2:154–65.
32.
MorenoJJ. Effect of olive oil minor components on oxidative stress and arachidonic acid mobilization and metabolism by macrophages raw. Radic Biol Med. 2003;35:1073–81.
33.
Gorzynik-DenbickaM, PrzychodzenP, CappelloF, Gorska-PonikiwskaM. Potential health benefits of olive oil and plant polyphenols. Int J Mol Sci. 2018;19:686.
34.
PrestiG, GuarrasiV, GulottaE, ProvenzanoF, ProvenzanoA, GiulianoS, et al.Bioactive compounds from extra virgin olive oils: Correlation between phenolic content and oxidative stress cell protection. Biophys Chem. 2017;230:109–16.
35.
EscrichE, SolanasM, MoralR. Modulatory effects and molecular mechanisms of olive oil and other dietary lipids in breast cancer. Curr Pharm Des. 2011;17:813–30.
36.
KrukI, Aboul-EneinHY, MichalskaT, LichszteldK, KładnaA. Scavenging of reactive oxygen species by the plant phenols genistein and oleuropein. Luminescence. 2005;20:81–9.
37.
CasadeyR, ChallierC, SenzA, CriadoS. Antioxidant ability of tyrosol and derivative-compounds in the presence of O2(1Δg)-species. Studies of synergistic antioxidant effect with commercial antioxidants. Food Chem. 2019;285:275–81.
38.
LiL, LuoW, QianY, ZhuW, QianJ, LiJ, et al.Luteolin protects against diabetic cardiomyopathy by inhibiting NF-κB-mediated inflammation and activating the Nrf2-mediated antioxidant responses. Phytomedicine. 2018;59:152774.
39.
Bezerra-FilhoCSM, BarbozaJN, SouzaMTS, SabryP, IsmailNSM, de SousaDP. Therapeutic potential of vanillin and its main metabolites to regulate the inflammatory response and oxidative stress. Mini Rev Med Chem. 2019;19:1.
40.
KonstantinidouV, KhymenetsO, FitoM, De La TorreR, AngladaR, DopazoA, et al.Characterization of human gene expression changes after olive oil ingestion: an exploratory approach. Folia Biol (Praha). 2009;55:85–91.
41.
PiroddiM, AlbiniA, FabianiR, GiovannelliL, LuceriC, NatellaF, et al.Nutrigenomics of extra-virgin olive oil: A review. Biofactors. 2017;2:17–41.
42.
LadeiroY, Zucman-RossiJ. Micro-RNA (miRNA) and cancer: the case of hepatocellular cancers. Medicine/Sciences. 2009;25:467–72.
43.
FujiiT, ShimadaK, AsanoA, et al.MicroRNA-331-3p suppresses cervical cancer cell proliferation and E6/E7 expression by targeting NRP2. Int J Mol Sci. 2016;17:1351.
44.
ZhangM, SongY and ZhaiF. ARFHPV E7 oncogene, lncRNA HOTAIR, miR-331-3p and its target, NRP2, form a negative feedback loop to regulate the apoptosis in the tumorigenesis in HPV positive cervical cancer. J Cell Biochem. 2018;119:4397–407.
