The role of cerebellum in coordination of somatic motor activity has been studied in detailed in various species. However, experimental and clinical studies have shown the involvement of the cerebellum with various visceral and cognitive functions via its vast connections with the central nervous system. The present study aims to define the cortical and subcortical and brain stem connections of the cerebellum via the superior (SCP) and middle (MCP) cerebellar peduncle using biotinylated dextran amine (BDA) and Fluoro-Gold (FG) tracer in Wistar albino rats. 14 male albino rats received 20–50-nl pressure injections of either FG or BDA tracer into the SCP and MCP. Following 7–10 days of survival period, the animals were processed according to the related protocol for two tracers. Labelled cells and axons were documented using light and fluorescence microscope. The SCP connects cerebellum to the insular and infralimbic cortices whereas, MCP addition to the insular cortex, it also connects cerebellum to the rhinal, primary sensory, piriform and auditory cortices. Both SCP and MCP connected the cerebellum to the ventral, lateral, posterior and central, thalamic nuclei. Additionally, SCP also connects parafasicular thalamic nucleus to the cerebellum. The SCP connects cerebellum to basal ganglia (ventral pallidum and clastrum) and limbic structures (amygdaloidal nuclei and bed nucleus of stria terminalis), however, the MCP have no connections with basal ganglia or limbic structures. Both the SCP and MCP densely connects cerebellum to various brainstem structures. Attaining the knowledge of the connections of the SCP and MCP is important for the diagnosis of lesions in the MCP and SCP and would deepen current understanding of the neuronal circuit of various diseases or lesions involving the SCP and MCP.
AkhlaghiH., CorbenL., Georgiou-KaristianisN., BranshawJ., StoreyE., DelatyckiM.B.et al., Superior cerebellar peduncle atrophy in Friedreich's ataxia correlates with disease symptoms, Cerebellum10 (2011), 81–87. doi:10.1007/s12311-010-0232-3.
2.
AlexanderM.P., GillinghamS., SchweizerT. and StussD.T., Cognitive impairments due to focal cerebellar injuries in adults, Cortex48 (2012), 980–990. doi:10.1016/j.cortex.2011.03.012.
3.
BombinI., ArangoC. and BuchananR.W., Significance and meaning of neurological signs in schizophrenia: Two decades later, Schizophr. Bull.31 (2005), 962–977. doi:10.1093/schbul/sbi028.
4.
BrodalP., Principles of organization of the monkey corticopontine projection, Brain Res.148 (1978), 214–218. doi:10.1016/0006-8993(78)90392-X.
5.
ChidaK., IadecolaC., UnderwoodM.D. and ReisD.I., A novel vasodepressor response elicited from the rat cerebellar fastigial nucleus: The fastigial depressor response, Brain Res.370 (1986), 378–382. doi:10.1016/0006-8993(86)90498-1.
6.
FerlandR.J., EyaidW., ColluraR.V., TullyL.D., HillR.S., Al-NouriD.et al., Abnormal cerebellar development and axonal decussation due to mutations in AHI1 in Joubert syndrome, Nat. Genet.36 (2004), 1008–1013. doi:10.1038/ng1419.
7.
HainesD.E., DietrichsE., MihailoffG.A. and McdonaldE.F., The cerebellar-hypothalamic axis: Basic circuits and clinical observations, Int. Rev. Neurobiol.41 (1997), 83–107.
8.
HolmesM.J., CotterL.A., ArendtH.E., CassS.P. and YatesB.J., Effects of lesions of the caudal cerebellar vermis on cardiovascular regulation in awake cats, Brain Res.31 (2002), 62–72. doi:10.1016/S0006-8993(02)02495-2.
9.
KataokaH., TonomuraY., TaokaT. and UenoS., Signal changes of superior cerebellar peduncle on fluid-attenuated inversion recovery in progressive supranuclear palsy, Parkinson Relat. Disord.4 (2008), 63–65. doi:10.1016/j.parkreldis.2007.03.001.
10.
LadabaumU., MinoshimaS., HaslerW.L., CrosD., CheyW.D. and OwyangC., Gastric distention correlates with activation of multiple cortical and subcortical regions, Gastroenterology120 (2001), 369–376. doi:10.1053/gast.2001.21201.
11.
LewisJ.L., Lo TurcoJ.J. and SolomonP.R., Lesions of the middle cerebellar peduncle disrupt acquisition and retention of the rabbit's classically conditioned nictitating membrane response, Behav. Neurosci.101 (1987), 151–157. doi:10.1037/0735-7044.101.2.151.
MillerR.A. and StromingerN.L., An experimental study of the efferent connections of the superior cerebellar peduncle in the rhesus monkey, Brain Res.16 (1977), 237–250. doi:10.1016/0006-8993(77)90761-2.
14.
NaidichT., DuvernoyH., DelmanB., SorensenA., KolliasS. and HaackeE., Duvernoy's Atlas of the Human Brain Stem and Cerebellum: High-Field MRI, Surface Anatomy, Internal Structure, Vascularization and 3 D Sectional Anatomy, Springer, Vienna, 2009.
15.
NicolettiG., TononC., LodiR., CondinoF., MannersD., MalucelliE.et al., Apparent diffusion of the superior cerebellar peduncle differentiates progressive supranuclear palsy from Parkinson's disease, Mov. Disord.15 (2008), 2370–2376. doi:10.1002/mds.22279.
16.
OkaM., KatayamaS., ImonY., OhshitaT., MimoriY. and NakamuraS., Abnormal signals on proton density-weighted MRI of the superior cerebellar peduncle in progressive supranuclear palsy, Acta Neurol. Scand.104 (2001), 1–5. doi:10.1034/j.1600-0404.2001.00262.x.
17.
OkugawaG., NobuharaK., MinamiT., TakaseK., SugimotoT., SaitoY.et al., Neural disorganization in the superior cerebellar peduncle and cognitive abnormality in patients with schizophrenia: A diffusion tensor imaging study, Prog. Neuropsychopharmacol. Biol. Psychiatry30 (2006), 1408–1412. doi:10.1016/j.pnpbp.2006.05.014.
18.
PaganiE., GinestroniA., Della NaveR., AgostaF., SalviF., De MicheleG.et al., Assessment of brain white matter fiber bundle atrophy in patients with Friedreich ataxia, Radiology25 (2010), 882–889. doi:10.1148/radiol.10091742.
19.
ParentA., Carpenter's Human Neuroanatomy, 9th edn, Williams & Wilkins, Baltimore, MD, 1996, pp. 612–613.
20.
ParisiM.A., PinterJ.D., GlassI.A., FieldK., MariaB.L., ChanceP.F.et al., Cerebral and cerebellar motor activation abnormalities in a subject with Joubert syndrome: Functional magnetic resonance imaging (MRI) study, J. Child Neurol.19 (2004), 214–218.
21.
PaviourD.C., PriceS.L., StevensJ.M., LeesA.J. and FoxN.C., Quantitative MRI measurement of superior cerebellar peduncle in progressive supranuclear palsy, Neurology64 (2005), 675–679. doi:10.1212/01.WNL.0000151854.85743.C7.
22.
PicardH., AmadoI., Mouchet-MagesS., OlieJ. and KrebsM., The role of the cerebellum in schizophrenia: An update of clinical, cognitive, and functional evidences, Schizophr. Bull.34 (2008), 155–172. doi:10.1093/schbul/sbm049.
23.
PorettiA., BoltshauserE., LoennekerT., ValenteE.M., BrancatiF., Il'yasovK.et al., Diffusion tensor imaging in Joubert syndrome, AJNR Am. J. Neuroradiol.28 (2007), 1929–1933. doi:10.3174/ajnr.A0703.
24.
PreziosaP., RoccaM.A., MesarosS., PaganiE., DrulovicJ., Stosic-OpincalT.et al., Relationship between damage to the cerebellar peduncles and clinical disability in multiple sclerosis, Radiology271 (2014), 822–830. doi:10.1148/radiol.13132142.
25.
QuislingR.G., BarkovichA.J. and MariaB., Magnetic resonance imaging features and classification of central nervous system malformations in Joubert syndrome, J. Child Neurol.14 (1999), 628–635. doi:10.1177/088307389901401002.
26.
SasakiK., OkaH., MatsudaY., ShimonoT. and MizunoN., Electrophysiological studies of the projections from the parietal association area to the cerebellar cortex, Exp. Brain Res.23 (1975), 91–102. doi:10.1007/BF00238732.
27.
SpampinatoM.V., KraasJ., MariaB.L., WaltonZ.J. and RumboldtZ., Absence of decussation of the superior cerebellar peduncles in patients with Joubert syndrome, Am. J. Med. Genet. A146A (2008), 1389–1394.
28.
ThürlingM., HautzelH., KüperM., StefanescuM.R., MaderwaldS., LaddM.E.et al., Involvement of the cerebellar cortex and nuclei in verbal and visuospatial working memory: A 7T fMRI study, Neuroimage62 (2012), 1537–1550. doi:10.1016/j.neuroimage.2012.05.037.
29.
TravisK.E., LeitnerY., FeldmanH.M. and Ben-ShacharM., Cerebellar white matter pathways are associated with reading skills in children and adolescents, Hum. Brain Mapp.36 (2015), 1536–1553. doi:10.1002/hbm.22721.
30.
TsuboiY., SlowinskiJ., JosephsK.A., HonerW.G., WszolekZ.K. and DicksonD.W., Atrophy of superior cerebellar peduncle in progressive supranuclear palsy, Neurology60 (2003), 1766–1769. doi:10.1212/01.WNL.0000068011.21396.F4.
31.
UchinoA., SawadaA., TakaseY. and KudoS., Symmetrical lesions of the middle cerebellar peduncle: MR imaging and differential diagnosis, Magn. Reson. Med. Sci.15 (2004), 133–140. doi:10.2463/mrms.3.133.
32.
WangF., SunZ., DuX., WangX., CongZ. and ZhangH.et al., A diffusion tensor imaging study of middle and superior cerebellar peduncle in male patients with schizophrenia, Neurosci. Lett.48 (2003), 35–38.
33.
XuF. and FrazierD.T., Role of the cerebellar deep nuclei in respiratory modulation, Cerebellum1 (2002), 35–40. doi:10.1080/147342202753203078.
34.
ZhengZ.H., DietrichsE. and WalbergF., Cerebellar afferent fibres from the dorsal vagal nucleus in the cat, Neurosci. Lett.32 (1982), 113–118. doi:10.1016/0304-3940(82)90259-2.
35.
ZhuJ.N., YungW.H., Kwok-Chong ChowB., ChanY.S. and WangJ.J., The cerebellar-hypothalamic circuits: Potential pathways underlying cerebellar involvement in somatic-visceral integration, Brain Res. Rev.52 (2006), 93–106. doi:10.1016/j.brainresrev.2006.01.003.
36.
ZhuangJ., XuF. and FrazierD.T., Hyperventilation evoked by activation of the vicinity of the caudal inferior olivary nucleus depends on the fastigial nucleus in anesthetized rats, J. Appl. Physiol.4 (2008), 1351–1358. doi:10.1152/japplphysiol.00824.2007.
