Colorectal cancer (CRC) is one of the most common cancers in the world; therefore, extensive research is needed to find new molecular therapeutic targets and biomarkers. LncRNA (long non-coding RNA), a new class of non-coding RNAs, has a crucial role in the onset and progression of various cancersincluding colorectal cancer. Research on lncRNA is still at initial stages and underlying molecular mechanisms of the vast majority of lncRNA have remained unclear. LOC100287225 is one of these novel lncRNAs (long intergenic non-coding RNA) located in the long arm of the chromosome 18. The purpose of this study was to determine the expression of LOC100287225 in colorectal tissue, and its misregulation in CRC patients. Quantitativereal-time-PCR (qRT-PCR) was used to investigate the LOC100287225 expression in pairs of tumorous and adjacent tumor-free tissues of 39 colorectal cancer patients. Also, the relationship between the clinicopathology and expression of LOC100287225 was determined. QRT-PCR results revealed that not only is LOC100287225 expressed in the intestinal tissue, but has also been misregulated during tumorigenesis. Moreover, LOC100287225 RNA relative expression levels were significantly lower in tumor tissues compared with adjacent tumor-free tissues (P< 0.001). RNA expression level of LOC100287225 did not show significant correlation with clinical characteristics. In conclusion, our study demonstrated that LOC100287225 misregulation couldbe a potential target for gene therapy in colorectal cancer.
Da SaccoL, BaldassarreA, MasottiA. Bioinformatics Tools and Novel Challenges in Long Non-Coding RNAs (lncRNAs) Functional Analysis. International journal of molecular sciences. 2012; 13(1): 97-114. doi: 10.3390/ijms13010097.
2.
BrosnanCA, VoinnetO. The long and the short of noncoding RNAs. Current opinion in cell biology. 2009; 21(3): 416-25.
3.
MattickJS. The Genetic Signatures of Noncoding RNAs. PLoS Genet. 2009; 5(4): e1000459. doi: 10.1371/journal.pgen.1000459.g001.
4.
MattickJS. A new paradigm for developmental biology. The Journal of experimental biology. 2007; 210(Pt 9): 1526-47. doi: 10.1242/jeb.005017.
5.
GibbEA, VucicEA, EnfieldKS, StewartGL, LonerganKM, KennettJY et al. Human cancer long non-coding RNA transcriptomes. PLoS One. 2011; 6(10): e25915.
6.
HauptmanN, GlavačD. Long non-coding RNA in cancer. International journal of molecular sciences. 2013; 14(3): 4655-69.
7.
HanL, ZhangK, ShiZ, ZhangJ, ZhuJ, ZhuS et al. LncRNA pro fi le of glioblastoma reveals the potential role of lncRNAs in contributing to glioblastoma pathogenesis. International journal of oncology. 2012; 40(6): 2004-12. doi: 10.3892/ijo.2012.1413.
8.
GutschnerT, DiederichsS. The hallmarks of cancer A long non-coding RNA point of view. RNA biology. 2012; 9(6): 703-19.
9.
PonjavicJ, OliverPL, LunterG, PontingCP. Genomic and transcriptional co-localization of protein-coding and long non-coding RNA pairs in the developing brain. PLoS Genet. 2009; 5(8): e1000617. doi: 10.1371/journal.pgen.1000617.
10.
YangL, FrobergJE, LeeJT. Long noncoding RNAs: fresh perspectives into the RNA world. Trends in biochemical sciences. 2014; 39(1): 35-43. doi: 10.1016/j.tibs.2013.10.002.
11.
ZhangQ, JeangK-T. Long noncoding RNAs and viral infections. BioMedicine. 2013; 3(1): 34-42. doi: 10.1016/j.biomed.2013.01.001.
12.
WangX, SongX, GlassCK, RosenfeldMR. The Long Arm of Long Noncoding RNAs: Roles as Sensors Regulating Gene Transcriptional Programs. Cold Spring Harb Perspect Biol. 2011; 3: a003756. doi: 10.1101/cshperspect.a003756.
13.
BhanA, HussainI, AnsariKI, KasiriS, BashyalA, MandalSS. Antisense transcript long noncoding RNA (lncRNA) HOTAIR is transcriptionally induced by estradiol. J Mol Biol. 2013; 425(19): 3707-22. doi: 10.1016/j.jmb.2013.01.022.
14.
PrensnerJR, ChinnaiyanAM. The emergence of lncRNAs in cancer biology. Cancer discovery. 2011; 1(5): 391-407. doi: 10.1158/2159-8290.CD-11-0209.
15.
ClarkMB, MattickJS, editors. Long noncoding RNAs in cell biology. Seminars in cell & developmental biology; 2011: Elsevier.
16.
NovikovaIV, HennellySP, SanbonmatsuKY. Sizing up long non-coding RNAs: do lncRNAs have secondary and tertiary structure?Bioarchitecture. 2012; 2(6): 189-99. doi: 10.4161/bioa.22592.
17.
NieL, WuHJ, HsuJM, ChangSS, LabaffAM, LiCW et al. Long non-coding RNAs: versatile master regulators of gene expression and crucial players in cancer. Am J Transl Res. 2012; 4(2): 127-50.
18.
KhachaneAN, HarrisonPM. Mining mammalian transcript data for functional long non-coding RNAs. PLoS One. 2010; 5(4): e10316. doi: 10.1371/journal.pone.0010316.
19.
NgSY, JohnsonR, StantonLW. Human long non-coding RNAs promote pluripotency and neuronal differentiation by association with chromatin modifiers and transcription factors. The EMBO journal. 2012; 31(3): 522-33. doi: 10.1038/emboj.2011.459.
20.
LiCH, ChenY. Targeting long non-coding RNAs in cancers: progress and prospects. Int J Biochem Cell Biol. 2013; 45(8): 1895-910. doi: 10.1016/j.biocel.2013.05.030.
21.
KogoR, ShimamuraT, MimoriK, KawaharaK, ImotoS, SudoT et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer Res. 2011; 71(20): 6320-6. doi: 10.1158/0008-5472.can-11-1021.
22.
GrahamLD, PedersenSK, BrownGS, HoT, KassirZ, MoynihanAT et al. Colorectal Neoplasia Differentially Expressed (CRNDE), a Novel Gene with Elevated Expression in Colorectal Adenomas and Adenocarcinomas. Genes & cancer. 2011; 2(8): 829-40. doi: 10.1177/1947601911431081.
23.
XuC, YangM, TianJ, WangX, LiZ. MALAT-1: a long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis. International journal of oncology. 2011; 39(1): 169-75. doi: 10.3892/ijo.2011.1007.
24.
SiegelR, MaJ, ZouZ, JemalA. Cancer statistics, 2014. CA: A cancer journal for clinicians. 2014; 64(1): 9-29.
25.
National Center for Biotechnology Information (NCBI). LOC100287225 uncharacterized LOC100287225, Homo sapiens (human). http://www.ncbi.nlm.nih.gov/gene/?term= LOC100287225.
26.
National Center for Biotechnology Information (NCBI). UniGene, Uncharacterized LOC100287225 (LOC100287225). http://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs& CID=448920.
27.
HanY, YangYN, YuanHH, ZhangTT, SuiH, WeiXL et al. UCA1, a long non-coding RNA up-regulated in colorectal cancer influences cell proliferation, apoptosis and cell cycle distribution. Pathology. 2014; 46(5): 396-401. doi: 10.1097/pat.0000000000000125.
28.
KornienkoAE, GuenzlPM, BarlowDP, PaulerFM. Gene regulation by the act of long non-coding RNA transcription. BMC biology. 2013; 11(1): 59.
29.
ChangPJ, BaHY. Long Noncoding RNAs: Cellular Address Codes in Development and Disease. Cell. 2013; 152: 1298-307. doi: 10.1016/j.cell.2013.02.012.
30.
HuarteM, RinnJL. Large non-coding RNAs: missing links in cancer?Human molecular genetics. 2010; 19(R2): R152-61. doi: 10.1093/hmg/ddq353.
31.
XiangJF, YinQF, ChenT, ZhangY, ZhangXO, WuZ et al. Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus. Cell research. 2014; 24(5): 513-31. doi: 10.1038/cr.2014.35.
32.
IshibashiM, KogoR, ShibataK, SawadaG, TakahashiY, KurashigeJ et al. Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma. Oncology reports. 2013; 29(3): 946-50. doi: 10.3892/or.2012.2219.
33.
ZhengHT, ShiDB, WangYW, LiXX, XuY, TripathiP et al. High expression of lncRNA MALAT1 suggests a biomarker of poor prognosis in colorectal cancer. International journal of clinical and experimental pathology. 2014; 7(6): 3174-81.
34.
YanB, GuW, YangZ, GuZ, YueX, GuQ et al. Downregulation of a long noncoding RNA-ncRuPAR contributes to tumor inhibition in colorectal cancer. Tumour biology: the journal of the International Society for Oncodevelopmental Biology and Medicine. 2014; 35(11): 11329-35. doi: 10.1007/s13277-014-2465-0.
35.
QiP, XuMD, NiSJ, HuangD, WeiP, TanC et al. Low expression of LOC285194 is associated with poor prognosis in colorectal cancer. Journal of translational medicine. 2013; 11: 122. doi: 10.1186/1479-5876-11-122.
36.
LiuQ, HuangJ, ZhouN, ZhangZ, ZhangA, LuZ et al. LncRNA loc285194 is a p53-regulated tumor suppressor. Nucleic Acids Res. 2013; 41(9): 4976-87. doi: 10.1093/nar/gkt182.
37.
BaderS, WalkerM, McQueenHA, SellarR, OeiE, WopereisS et al. MBD1, MBD2 and CGBP genes at chromosome 18q21 are infrequently mutated in human colon and lung cancers. Oncogene. 0000; 22(22): 3506-10.
38.
DerksS, BoschLJ, NiessenHE, MoerkerkPT, van den BoschSM, CarvalhoB et al. Promoter CpG island hypermethylation- and H3K9me3 and H3K27me3-mediated epigenetic silencing targets the deleted in colon cancer (DCC) gene in colorectal carcinogenesis without affecting neighboring genes on chromosomal region 18q21. Carcinogenesis. 2009; 30(6): 1041-8. doi: 10.1093/carcin/bgp073.
39.
Martinez-LopezE, AbadA, FontA, MonzoM, OjangurenI, PifarreA et al. Allelic loss on chromosome 18q as a prognostic marker in stage II colorectal cancer. Gastroenterology. 1998; 114(6): 1180-7.
40.
CipollaGA. A non-canonical landscape of the microRNA system. Frontiers in genetics. 2014; 5: 337. doi: 10.3389/fgene.2014.00337.
41.
ComuzzieAG, ColeSA, LastonSL, VorugantiVS, HaackK, GibbsRA et al. Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population. PLoS One. 2012; 7(12): e51954. doi: 10.1371/journal.pone.0051954.
