Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites
Restricted accessResearch articleFirst published online January, 2019
Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites
The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary. Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
OBJECTIVE:
The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
METHODS:
The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days. The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy. Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
RESULTS:
The results show a porous and interconnected morphology with enhanced bioactivity. It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds.
CONCLUSIONS:
The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
ZhangD.WuX.ChenJ. and LinK., The development of collagen based composite scaffolds for bone regeneration, Bioactive Materials3(1) (2018), 129–138.
2.
BoseS.RoyM. and BandyopadhyayA., Recent advances in bone tissue engineering scaffolds, Trends in Biotechnology30(10) (2012), 546–554.
3.
CorreiaT.R.FigueiraD.R.de SáK.D.MiguelS.P.FradiqueR.G.MendonçaA.G., 3D printed scaffolds with bactericidal activity aimed for bone tissue regeneration, International Journal of Biological Macromolecules.
4.
StiersP.-J.van GastelN. and CarmelietG., Targeting the hypoxic response in bone tissue engineering: A balance between supply and consumption to improve bone regeneration, Molecular and Cellular Endocrinology432 (2016), 96–105.
5.
Martinez-SanzE.OssipovD.A.HilbornJ.LarssonS.JonssonK.B. and VargheseO.P., Bone reservoir: Injectable hyaluronic acid hydrogel for minimal invasive bone augmentation, Journal of Controlled Release: Official Journal of the Controlled Release Society152(2) (2011), 232–240.
6.
Rajan UnnithanA.Ramachandra Kurup SasikalaA.ParkC.H. and KimC.S., A unique scaffold for bone tissue engineering: An osteogenic combination of graphene oxide–hyaluronic acid–chitosan with simvastatin, Journal of Industrial and Engineering Chemistry46 (2017), 182–191.
7.
SharmaC.DindaA.K.PotdarP.D.ChouC.-F. and MishraN.C., Fabrication and characterization of novel nano-biocomposite scaffold of chitosan–gelatin–alginate–hydroxyapatite for bone tissue engineering, Materials Science and Engineering: C64 (2016), 416–427.
8.
SharifiF.KurteshiD. and HashemiN., Designing highly structured polycaprolactone fibers using microfluidics, Journal of the Mechanical Behavior of Biomedical Materials61 (2016), 530–540.
9.
Plazas BonillaC.E.TrujilloS.DemirdögenB.PerillaJ.E.Murat ElcinY. and Gómez RibellesJ.L., New porous polycaprolactone–silica composites for bone regeneration, Materials Science and Engineering: C40 (2014), 418–426.
10.
WoodruffM.A. and HutmacherD.W., The return of a forgotten polymer—Polycaprolactone in the 21st century, Progress in Polymer Science35(10) (2010), 1217–1256.
11.
TranP.A.ZhangL. and WebsterT.J., Carbon nanofibers and carbon nanotubes in regenerative medicine, Advanced Drug Delivery Reviews61(12) (2009), 1097–1114.
12.
WendeF.J.GohilS.MojarradiH.GerfaudT.NordL.I.KarlssonA., Determination of substitution positions in hyaluronic acid hydrogels using NMR and MS based methods, Carbohydrate Polymers136 (2016), 1348–1357.
13.
KutlusoyT.OktayB.ApohanN.K.SuleymanogluM. and KurucaS.E., Chitosan-co-Hyaluronic acid porous cryogels and their application in tissue engineering, International Journal of Biological Macromolecules103 (2017), 366–378.
14.
HemshekharM.ThusharaR.M.ChandranayakaS.ShermanL.S.KemparajuK. and GirishK.S., Emerging roles of hyaluronic acid bioscaffolds in tissue engineering and regenerative medicine, International Journal of Biological Macromolecules86 (2016), 917–928.
15.
NecasJ.BartosikovaL.BraunerP. and KolarJ., Hyaluronic acid (hyaluronan): a review, Veterinarni Medicina53(8) (2008), 397–4911.
16.
StolzenbachS.BredieW.L.P. and ByrneD.V., Consumer concepts in new product development of local foods: Traditional versus novel honeys, Food Research International52(1) (2013), 144–152.
17.
ValverdeT.M.CastroE.G.CardosoM.H.Martins-JuniorP.A.SouzaL.M.SilvaP.P., A novel 3D bone-mimetic scaffold composed of collagen/MTA/MWCNT modulates cell migration and osteogenesis, Life Sci.162 (2016), 115–124.
18.
HirataE.UoM.TakitaH.AkasakaT.WatariF. and YokoyamaA., Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering, Carbon49(10) (2011), 3284–3291.
19.
WarowickaA.MaciejewskaB.M.LitowczenkoJ.KościńskiM.Baranowka-KorczycA.Jasiurkowska-DelaporteM., MWCNT based matrices as a platform for adhesion and growth of cells, Composites Science and Technology136 (2016), 29–38.
20.
ChakrabortyR.SeesalaV.S.SenM.SenguptaS.DharaS.SahaP., MWCNT reinforced bone like calcium phosphate—Hydroxyapatite composite coating developed through pulsed electrodeposition with varying amount of apatite phase and crystallinity to promote superior osteoconduction, cytocompatibility and corrosion protection performance compared to bare metallic implant surface, Surface and Coatings Technology325 (2017), 496–514.
21.
GholizadehS.MoztarzadehF.HaghighipourN.GhazizadehL.BaghbaniF.ShokrgozarM.A., Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/𝛽-Glycerophosphate scaffolds for bone tissue engineering, International Journal of Biological Macromolecules97 (2017), 365–372.
22.
LeOliveiraL.M.B.MacedoI.T.F.VieiraL.S.Camurça-VasconcelosA.L.F.ToméA.R.SampaioR.A., Effects of Mimosa tenuiflora on larval establishment of Haemonchus contortus in sheep, Veterinary Parasitology196(3–4) (2013), 341–346.
23.
ErkanG.ŞengülK. and KayaS., Dyeing of white and indigo dyed cotton fabrics with Mimosa tenuiflora extract, Journal of Saudi Chemical Society (2011).
24.
Martel-EstradaS.A.Olivas-ArmendárizI.Santos-RodríguezE.Martínez-PérezC.A.García-CasillasP.E.Hernández-PazJ., Evaluation of in vitro bioactivity of Chitosan/Mimosa tenuiflora composites, Materials Letters119 (2014), 146–149.
25.
Martel-EstradaS.A.Martínez-PérezC.A.Chacón-NavaJ.G.García-CasillasP.E. and Olivas–ArmendárizI., In vitro bioactivity of chitosan/poly (d,l-lactide-co-glycolide) composites, Materials Letters65(1) (2011), 137–141.
26.
Olivas ArmendárizI.Santos-RodríguezE.Alvarado-GutiérrezM.L.Meléndez-MolinaZ.A.Márquez-ChávezL.A.Valencia-GómezL.E.Vargas-RequenaC.L. and Martel-EstradaS.A., Biocomposites scaffolds for bone tissue engineering, International Journal of Composite Materials5(6) (2015), 167–176.
27.
Martinez-DiazS.Garcia-GiraltN.LebourgM.Gomez-TejedorJ.A.VilaG.CaceresE., In vivo evaluation of 3-dimensional polycaprolactone scaffolds for cartilage repair in rabbits, Am J Sports Med.38(3) (2010), 509–519.
28.
JensenJ.KraftD.C.LysdahlH.FoldagerC.B.ChenM.KristiansenA.A., Functionalization of polycaprolactone scaffolds with hyaluronic acid and beta-TCP facilitates migration and osteogenic differentiation of human dental pulp stem cells in vitro, Tissue Eng Part A21(3–4) (2015), 729–739.
29.
ZouL.ZouX.ChenL.LiH.MygindT.KassemM., Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro, Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society26(5) (2008), 713–720.
30.
LebourgM.Martinez-DiazS.Garcia-GiraltN.Torres-ClaramuntR.Gomez-TejedorJ.A.RibellesJ.L., Cell-free cartilage engineering approach using hyaluronic acid-polycaprolactone scaffolds: a study in vivo, J Biomater Appl.28(9) (2014), 1304–1315.
31.
SáM.A.RibeiroH.J.ValverdeT.M.SousaB.R.Martins-JúniorP.A.MendesR.M., Single-walled carbon nanotubes functionalized with sodium hyaluronate enhance bone mineralization, Brazilian Journal of Medical and Biological Research49(2) (2016).
32.
Olivas-ArmendarizI.Martel-EstradaS.A.Mendoza-DuarteM.E.Jiménez-VegaF.García-CasillasP. and Martínez-PérezC.A., Biodegradable chitosan/multiwalled carbon nanotube composite for bone tissue engineering, Journal of Biomaterials and Nanobiotechnology04(02) (2013), 204–211.
33.
Olivas-ArmendárizI.García-CasillasP.Martínez-SánchezR.Martínez-VillafañeA. and Martínez-PérezC.A., Chitosan/MWCNT composites prepared by thermal induced phase separation, Journal of Alloys and Compounds495(2) (2010), 592–595.
34.
Valencia-GomezL.E.Martel-EstradaS.A.Vargas-RequenaC.Rivera-ArmentaJ.L.Alba-BaenaN.Rodriguez-GonzalezC., Chitosan/Mimosa tenuiflora films as potential cellular patch for skin regeneration, International Journal of Biological Macromolecules93(Pt A) (2016), 1217–1225.
35.
HuY.ChenJ.FanT.ZhangY.ZhaoY.ShiX., Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nano-hydroxyapatite/chitosan/chondroitin sulfate/hyaluronic acid for bone tissue engineering, Colloids and Surfaces B: Biointerfaces157 (2017), 93–100.
36.
SiqueiraI.A.W.B.de MouraN.K.de Barros MachadoJ.P.BackesE.H.Roberto PassadorF. and de Sousa TrichêsE., Porous membranes of the polycaprolactone (PCL) containing calcium silicate fibers for guided bone regeneration, Materials Letters206 (2017), 210–213.
37.
FigueiraD.R.MiguelS.P.de SaK.D. and CorreiaI.J., Production and characterization of polycaprolactone-hyaluronic acid/chitosan-zein electrospun bilayer nanofibrous membrane for tissue regeneration, International Journal of Biological Macromolecules93(Pt A) (2016), 1100–1110.
38.
JonesA.C.ArnsC.H.HutmacherD.W.MilthorpeB.K.SheppardA.P. and KnackstedtM.A., The correlation of pore morphology, interconnectivity and physical properties of 3D ceramic scaffolds with bone ingrowth, Biomaterials30(7) (2009), 1440–1451.
39.
HeF.-L.LiD.-W.HeJ.LiuY.-Y.AhmadF.LiuY.-L., A novel layer-structured scaffold with large pore sizes suitable for 3D cell culture prepared by near-field electrospinning, Materials Science and Engineering: C86 (2018), 18–27.
40.
SionkowskaA. and KaczmarekB., Preparation and characterization of composites based on the blends of collagen, chitosan and hyaluronic acid with nano-hydroxyapatite, International Journal of Biological Macromolecules102 (2017), 658–666.
41.
Martel-EstradaS.A.Olivas-ArmendarizI.Martinez-PerezC.A.HernandezT.Acosta-GomezE.I.Chacon-NavaJ.G., Chitosan/poly(DL,lactide-co-glycolide) scaffolds for tissue engineering, Journal of Materials Science Materials in Medicine23(12) (2012), 2893–2901.
42.
FadaieM.MirzaeiE.GeramizadehB. and AsvarZ., Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties, Carbohydrate Polymers199 (2018), 628–640.
43.
ChandaA.AdhikariJ.GhoshA.ChowdhuryS.R.ThomasS.DattaP., Electrospun chitosan/polycaprolactone-hyaluronic acid bilayered scaffold for potential wound healing applications, International Journal of Biological Macromolecules116 (2018), 774–785.
44.
NewmanP.MinettA.Ellis-BehnkeR. and ZreiqatH., Carbon nanotubes: their potential and pitfalls for bone tissue regeneration and engineering, Nanomedicine : Nanotechnology, Biology, and Medicine9(8) (2013), 1139–1158.
45.
Martel-EstradaS.A.Rodríguez-EspinozaB.Santos-RodríguezE.Jiménez-VegaF.García-CasillasP.E.Martínez-PérezC.A., Biocompatibility of chitosan/Mimosa tenuiflora scaffolds for tissue engineering, Journal of Alloys and Compounds643 (2015), S119–S123.
46.
WopenkaB. and PasterisJ., A mineralogical perspective on the apatite in bone, Materials Science & Engineering C25 (2005), 131–143.
47.
MukherjeeS.KunduB.SenS. and ChandaA., Improved properties of hydroxyapatite–carbon nanotube biocomposite: Mechanical, in vitro bioactivity and biological studies, Ceramics International40(4) (2014), 5635–5643.
48.
ShresthaB.K.ShresthaS.TiwariA.P.KimJ.-I.KoS.W.KimH.-J., Bio-inspired hybrid scaffold of zinc oxide-functionalized multi-wall carbon nanotubes reinforced polyurethane nanofibers for bone tissue engineering, Materials & Design133 (2017), 69–81.
