In vitro evaluation of collagen immobilization on polytetrafluoroethylene through NH 3 plasma treatment to enhance endothelial cell adhesion and growth

Abstract

Background:

Polytetrafluoroethylene (PTFE) is poorly biocompatible due to its low surface energy and hydrophobicity, which cause weak cell attachment and proliferation and complicate its use in implants.

Objective:

NH₃ plasma was used for surface modification and binding of amine groups on the PTFE surface. Collagen was immobilized on the plasma-treated PTFE in order to enable it to support enhanced cell adhesion and growth.

Methods:

PTFE was exposed to NH₃ plasma and collagen was immobilized on the NH₃ plasma-treated surface. ATR-IR, SEM, EDXA and contact angle were conducted to determine the composition, microstructure and wettability of samples. The cytocompatibility of the samples was assessed via the growth HUVEC cells using MTT assay.

Results:

Plasma treatment resulted in an incorporation of functional groups, containing N₂ and O₂ that caused the PTFE surface to become hydrophilic with contact angle 68°. Also, a reduction in F/C ratio was observed after collagen immobilization that indicates the presence of collagen. Cells proliferated in greater numbers on the collagen immobilized-PTFE as compared to the plasma-treated one.

Conclusions:

Plasma treatment incorporates functional polar moieties on the PTFE surface, causing enhanced wettability, collagen immobilization and cell viability. Collagen-immobilized PTFE may offer a valuable solution in biomedical applications such as vessel grafts.

Keywords

Surface biological modifications collagen immobilization biocompatibility polytetrafluoroethylene artificial blood vessel hydrophilicity

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References

Zhang

and Feng

, Bibliometrics and visualization analysis of artificial blood vessel research, Curr Sci. 106 (2014), 816–822.

Zang

Zhang

Chen

Zhou

Chang

et al., Investigation on artificial blood vessels prepared from bacterial cellulose, Mater Sci Eng, C. 46 (2015), 111–117.

Toth

Klarik

Kiss

Toth

Hargitai

and Nemeth

, Early postoperative changes in hematological, erythrocyte aggregation and blood coagulation parameters after unilateral implantation of polytetrafluoroethylene vascular graft in the femoral artery of beagle dogs1, Acta Cir Bras. 29(5) (2014). doi:10.1590/S0102-86502014000500006.

Hoshi

R.A.

Van Lith

Jen

M.C.

Allen

J.B.

Lapidos

K.A.

and Ameer

, The blood and vascular cell compatibility of heparin-modified ePTFE vascular grafts, Biomaterials. 34(1) (2013), 30–41. doi:10.1016/j.biomaterials.2012.09.046.

Nemeno-Guanzon

J.G.

Lee

Berg

J.R.

Y.H.

Yeo

J.E.

Nam

B.M.

et al., Trends in tissue engineering for blood vessels, BioMed Research International. 2012 (2012).

Rothuizen

T.C.

Damanik

F.F.

Lavrijsen

Visser

M.J.

Hamming

J.F.

Lalai

R.A.

et al., Development and evaluation of in vivo tissue engineered blood vessels in a porcine model, Biomaterials. 75 (2016), 82–90. doi:10.1016/j.biomaterials.2015.10.023.

Barshilia

H.C.

and Gupta

, Superhydrophobic polytetrafluoroethylene surfaces with leaf-like micro-protrusions through AR+ O 2 plasma etching process, Vacuum. 99 (2014), 42–48. doi:10.1016/j.vacuum.2013.04.020.

Carbonell

Martín

I.S.

Santos

Pujol

Sanz-Moliner

and Nart

, High-density polytetrafluoroethylene membranes in guided bone and tissue regeneration procedures: A literature review, Int J Oral Maxillofac Surg. 43(1) (2014), 75–84. doi:10.1016/j.ijom.2013.05.017.

Chen

Zamora

P.O.

Som

Peña

L.A.

and Osaki

, Cell attachment and biocompatibility of polytetrafluoroethylene (PTFE) treated with glow-discharge plasma of mixed ammonia and oxygen, J Biomater Sci Polym Ed. 14(9) (2003), 917–935. doi:10.1163/156856203322381410.

10.

Yablokov

M.Y.

Shevchenko

Gilman

and Kuznetsov

, Dielectric properties of polytetrafluoroethylene films modified by direct current discharge, High Energ Chem. 48(4) (2014), 282–286. doi:10.1134/S0018143914040134.

11.

Ahad

I.U.

Butruk

Ayele

Budner

Bartnik

Fiedorowicz

et al., Extreme ultraviolet (EUV) surface modification of polytetrafluoroethylene (PTFE) for control of biocompatibility, Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 364 (2015), 98–107. doi:10.1016/j.nimb.2015.08.093.

12.

Jaganathan

S.K.

Supriyanto

Murugesan

Balaji

and Asokan

M.K.

, Biomaterials in cardiovascular research: Applications and clinical implications, BioMed Research International. 2014 (2014).

13.

Zhu

Yuan

Hock Yeo

and Nakao

, Thermal treatment of expanded polytetraflu-oroethylene (ePTFE) membranes for reconstruction of a valved conduit1, Bio-Medical Materials and Engineering. 26(1) (2015), S55–S62.

14.

Yamashita

Suzuki

Tabayashi

Abe

Hayata

Hirose

et al., Experimental use of crosslinked gelatin glue for arterial hemostasis in cardiovascular surgery, Bio-Medical Materials and Engineering. 25(4) (2015), 361–370. doi:10.3233/BME-151534.

15.

Onder

Kazmanli

and Kok

F.N.

, Alteration of PTFE surface to increase its blood compatibility, J Biomater Sci Polym Ed. 22(11) (2011), 1443–1457. doi:10.1163/092050610X510551.

16.

Beimers

Lam

P.H.

and Murrell

G.A.

, The biomechanical effects of polytetrafluoroethylene suture augmentations in lateral-row rotator cuff repairs in an ovine model, J Shoulder Elbow Surg 23(10) (2014), 1545–1552. doi:10.1016/j.jse.2014.02.003.

17.

Michel

E.C.

Montaño-Machado

Chevallier

Labbé-Barrère

Letourneur

and Mantovani

, Dextran grafting on PTFE surface for cardiovascular applications, Biomatter. 4(1) (2014), F28805. doi:10.4161/biom.28805.

18.

Montaño-Machado

Chevallier

Mantovani

and Pauthe

, On the potential for fibronectin/phosphorylcholine coatings on PTFE substrates to jointly modulate endothelial cell adhesion and hemocompatibility properties, Biomatter. 5(1) (2015), e979679.

19.

Vaz

J.M.

Michel

E.C.

Chevallier

Beppu

M.M.

and Mantovani

, Covalent grafting of chitosan on plasma-treated polytetrafluoroethylene surfaces for biomedical applications, Journal of Biomaterials and Tissue Engineering. 4(11) (2014), 915–924. doi:10.1166/jbt.2014.1262.

20.

Y.-H.

and Huang

Y.-D.

, The study of collagen immobilization on polyurethane by oxygen plasma treatment to enhance cell adhesion and growth, Surf Coat Technol 201(9) (2007), 5124–5127. doi:10.1016/j.surfcoat.2006.07.128.

21.

Cech

Knob

Hosein

H.-A.

Babik

Lepcio

Ondreas

et al., Enhanced interfacial adhesion of glass fibers by tetravinylsilane plasma modification, Composites Part A: Applied Science and Manufacturing. 58 (2014), 84–89. doi:10.1016/j.compositesa.2013.12.003.

22.

Cho

Y.K.

Park

Kim

Lee

Park

Kim

H.J.

et al., Bioactive surface modifications on inner walls of poly-tetra-fluoro-ethylene tubes using dielectric barrier discharge, Appl Surf Sci. 296 (2014), 79–85. doi:10.1016/j.apsusc.2014.01.048.

23.

Fabbri

Gigli

Costa

Govoni

Seri

Lotti

et al., The effect of plasma surface modification on the biodegradation rate and biocompatibility of a poly (butylene succinate)-based copolymer, Polym Degradation Stab 121 (2015), 271–279. doi:10.1016/j.polymdegradstab.2015.09.015.

24.

Kolská

Lupínková

Výborný

Kasálková

Slepičková

Nagyová

et al. (eds), Improvement of polymer surface cytocompatibility by plasma treatment and subsequent grafting of vicinal compounds, in: 20th Symposium on Application of Plasma Processes, 2015.

25.

Garcia-Torres

Sylla

Molina

Crespo

Mota

and Bautista

, Surface modification of cellulosic substrates via atmospheric pressure plasma polymerization of acrylic acid: Structure and properties, Appl Surf Sci. 305 (2014), 292–300. doi:10.1016/j.apsusc.2014.03.065.

26.

Dufour

Hubert

Viville

Duluard

C.Y.

Desbief

Lazzaroni

et al., PTFE surface etching in the post-discharge of a scanning RF plasma torch: Evidence of ejected fluorinated species, Plasma Processes and Polymers 9(8) (2012), 820–829. doi:10.1002/ppap.201100209.

27.

Wilsion

Williams

and Pond

, Plasma modification of PTFE surfaces, Surf Inter Anal. 31 (2001), 385. doi:10.1002/sia.1065.

28.

Kolská

Řezníčková

Hnatowicz

and Švorčík

, PTFE surface modification by Ar plasma and its characterization, Vacuum. 86(6) (2012), 643–647. doi:10.1016/j.vacuum.2011.07.015.

29.

Liu

Ren

Tong

Cui

et al., Comparative study on the effect of RF and DBD plasma treatment on PTFE surface modification, Mater Chem Phys. 85(2) (2004), 340–346. doi:10.1016/j.matchemphys.2004.01.026.

30.

Hunke

Soin

Shah

Kramer

Witan

and Siores

, Influence of plasma pre-treatment of polytetrafluoroethylene (PTFE) micropowders on the mechanical and tribological performance of polyethersulfone (PESU)–PTFE composites, Wear. 328–329 (2015), 480–487.

31.

Sarani

De Geyter

Nikiforov

A.Y.

Morent

Leys

Hubert

et al., Surface modification of PTFE using an atmospheric pressure plasma jet in argon and argon+ CO 2, Surf Coat Technol 206(8) (2012), 2226–2232. doi:10.1016/j.surfcoat.2011.09.070.

32.

Kozlowska

Sionkowska

Skopinska-Wisniewska

and Piechowicz

, Northern pike (Esox lucius) collagen: Extraction, characterization and potential application, Int J Biol Macromol. 81 (2015), 220–227. doi:10.1016/j.ijbiomac.2015.08.002.

33.

and Jiang

, Editorial on the original article entitled “3D printing of composite calcium phosphate and collagen scaffolds for bone regeneration”, published in the Biomaterials on February 14, 2014. Annals of translational medicine. 2015; 3(Suppl 1).

34.

Ding

Zhang

and Li

, Fluorescence study on the aggregation of collagen molecules in acid solution influenced by hydroxypropyl methylcellulose, Carbohydr Polym. 136 (2016), 224–231. doi:10.1016/j.carbpol.2015.09.028.

35.

Tang

Ding

Min

Jiao

et al., Collagen films with stabilized liquid crystalline phases and concerns on osteoblast behaviors, Materials Science and Engineering: C. 58 (2016), 977–985. doi:10.1016/j.msec.2015.09.058.

36.

Wang

Chen

J.-R.

and Li

, Studies on surface modification of poly (tetrafluoroethylene) film by remote and direct Ar plasma, Appl Surf Sci. 254(9) (2008), 2882–2888. doi:10.1016/j.apsusc.2007.10.029.

37.

Tran

C.N.

and Walt

D.R.

, Plasma modification and collagen binding to PTFE grafts, J Colloid Interface Sci. 132(2) (1989), 373–381. doi:10.1016/0021-9797(89)90252-X.

38.

Mahmoodi

Hashemi

and Imani

, Fabrication of TiO2-Al2O3-HA Nanobiocomposite by Sintering and Evalution of in Vitro Bioactivity and Cell Toxicity for Orthopedic Applications.

39.

Hunke

Soin

Shah

T.H.

Kramer

Pascual

Karuna

M.S.L.

et al., Low-pressure H2, NH3 microwave plasma treatment of polytetrafluoroethylene (PTFE) powders: Chemical, thermal and wettability analysis, Materials. 8(5) (2015), 2258–2275. doi:10.3390/ma8052258.

40.

Sanchis

M.R.

Calvo

Fenollar

Garcia

and Balart

, Characterization of the surface changes and the aging effects of low-pressure nitrogen plasma treatment in a polyurethane film, Polym Test. 27(1) (2008), 75–83. doi:10.1016/j.polymertesting.2007.09.002.

41.

Dhayal

Alexander

M.R.

and Bradley

J.W.

, The surface chemistry resulting from low-pressure plasma treatment of polystyrene: The effect of residual vessel bound oxygen, Appl Surf Sci. 252(22) (2006), 7957–7963. doi:10.1016/j.apsusc.2005.10.005.

42.

Inagaki

Tasaka

and Kawai

, Improved adhesion of poly (tetrafluoroethylene) by NH3-plasma treatment, J Adhes Sci Technol. 3(1) (1989), 637–649. doi:10.1163/156856189X00461.

43.

Williams

Markkula

and Hunt

, Effects of plasma treated PET and PTFE on expression of adhesion molecules by human endothelial cells in vitro, Biomaterials. 23(11) (2002), 2411–2428. doi:10.1016/S0142-9612(01)00377-5.