Osteoblast-like cell responses to ion products released from magnesium- and silicate-containing calcium carbonates

Abstract

Background:

Inorganic ions released from bioceramics and bioactive glasses have been reported to influence osteogenic cell functions. Cell responses depend on types of the ions provided, for example, silicate ion has been found to up-regulate their proliferation, differentiation and mineralization.

Objective:

Mouse osteoblast-like cells (MC3T3-E1) were cultured in media containing silicate and calcium ions with/without magnesium ion to evaluate their combined effects on the cell’s functions.

Methods:

The cells were cultured in the media containing the extract of silicate-containing vaterite (SiV) and magnesium- and siloxane-containing one (MgSiV) and normal medium and then their adhesion, proliferation, differentiation and mineralization were evaluated.

Results:

The adhesion of the cells was enhanced when they were cultured in the medium containing MgSiV-extract. Their proliferation and differentiation were up-regulated in both media containing MgSiV-extract and SiV-extract. In particular, the MgSiV-extract significantly enhanced their differentiation than the SiV-extract. This was supported by the mineralization test’s results, which showed a large amount of mineral deposit was observed in the cells cultured in the MgSiV-extract medium.

Conclusions:

Providing the three kinds of ions was effective for up-regulating the cell’s mineralization compared to providing silicate and calcium ions without magnesium ion.

Keywords

Osteoblast-like cells mineralization inorganic ions calcium carbonate

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References

Hoppe

Güldal

N.S.

and Boccaccini

A.R.

, A review of the biological response to ionic dissolution products from bioactive glasses and glass–ceramics, Biomaterials32(11) (2011), 2757. doi:10.1016/j.biomaterials.2011.01.004.

Jell

and Stevens

M.M.

, Gene activation by bioactive glasses, J Mater Sci – Mater Med17(11) (2006), 997.

Xynos

I.D.

Edgar

A.J.

Buttery

L.D.K.

Hench

L.L.

and Polak

J.M.

, Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis, Biochem Biophys Res Commun276(2) (2000), 461. doi:10.1006/bbrc.2000.3503.

Xynos

I.D.

Hukkanen

M.V.J.

Batten

J.J.

Buttery

L.D.

Hench

L.L.

and Polak

J.M.

, Bioglass^®45S5 stimulates osteoblast turnover and enhances bone formation in vitro: Implications and applications for bone tissue engineering, Calcified Tissue Int67(4) (2000), 321. doi:10.1007/s002230001134.

Gough

J.E.

Jones

J.R.

and Hench

L.L.

, Osteoblast nodule formation and mineralisation on foamed 58S bioactive glass, Key Eng Mater254(256) (2004), 985. doi:10.4028/www.scientific.net/KEM.254-256.985.

Shie

M.-Y.

Ding

S.-J.

and Chang

H.-C.

, The role of silicon in osteoblast-like cell proliferation and apoptosis, Acta Biomater7(6) (2011), 2604. doi:10.1016/j.actbio.2011.02.023.

Maeno

Niki

Matsumoto

Morioka

Yatabe

Funayama

et al., The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture, Biomaterials26(23) (2005), 4847. doi:10.1016/j.biomaterials.2005.01.006.

Obata

Tokuda

and Kasuga

, Enhanced in vitro cell activity on silicon-doped vaterite/poly(lactic acid) composites, Acta Biomater5(1) (2009), 57. doi:10.1016/j.actbio.2008.08.004.

Nakamura

Poologasundarampillai

Jones

J.R.

and Kasuga

, Tracking the formation of vaterite particles containing aminopropyl-functionalized silsesquioxane and their structure for bone regenerative medicine, J Mater Chem B1(35) (2013), 4446. doi:10.1039/c3tb20589d.

10.

Obata

Hotta

Wakita

Ota

and Kasuga

, Electrospun microfiber meshes of silicon-doped vaterite/poly(lactic acid) hybrid for guided bone regeneration, Acta Biomater6(4) (2010), 1248. doi:10.1016/j.actbio.2009.11.013.

11.

Kasuga

Obata

Maeda

Ota

Yao

and Oribe

, Siloxane-poly(lactic acid)-vaterite composites with 3D cotton-like structure, J Mater Sci – Mater Med23(10) (2012), 2349. doi:10.1007/s10856-012-4607-5.

12.

Zreiqat

Howlett

C.R.

Zannettino

Evans

Schulze-Tanzil

Knabe

et al., Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants, J Biomed Mater Res62(2) (2002), 175. doi:10.1002/jbm.10270.

13.

Maier

J.A.M.

Bernardini

Rayssiguier

and Mazur

, High concentrations of magnesium modulate vascular endothelial cell behaviour in vitro, Biochim Biophys Acta1689(1) (2004), 6.

14.

Yamada

Ota

Nakamura

Sakka

and Kasuga

, Preparation of siloxane-containing vaterite doped with magnesium, J Ceram Soc Jpn122(1432) (2014), 1010. doi:10.2109/jcersj2.122.1010.

15.

Abed

and Moreau

, Importance of melastatin-like transient receptor potential 7 and magnesium in the stimulation of osteoblast proliferation and migration by platelet-derived growth factor, Am J Physiol – Cell Physiol297(2) (2009), C360. doi:10.1152/ajpcell.00614.2008.

16.

Owen

T.A.

Aronow

Shalhoub

Barone

L.M.

Wilming

Tassinari

M.S.

et al., Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix, J Cell Physiol143(3) (1990), 420. doi:10.1002/jcp.1041430304.

17.

Wiesmann

H.-P.

Tkotz

Joos

Zierold

Stratmann

Szuwart

et al., Magnesium in newly formed dentin mineral of rat incisor, J Bone Miner Res12(3) (1997), 380.

18.

Wuthier

R.E.

, Zonal analysis of phospholipids in the epiphyseal cartilage and bone of normal and rachitic chickens and pigs, Calcified Tissue Research8(1) (1971), 36. doi:10.1007/BF02010121.

19.

Leidi

Dellera

Mariotti

and Maier

J.A.M.

, High magnesium inhibits human osteoblast differentiation in vitro, Mgnes Res24(1) (2011), 1.

20.

Serre

C.M.

Papillard

Chavassieux

Voegel

J.C.

and Boivin

, Influence of magnesium substitution on a collagen–apatite biomaterial on the production of a calcifying matrix by human osteoblasts, J Biomed Mater Res42(4) (1998), 626. doi:10.1002/(SICI)1097-4636(19981215)42:4<626::AID-JBM20>3.0.CO;2-S.

21.

Yoshizawa

Brown

Barchowsky

and Sfeir

, Magnesium ion stimulation of bone marrow stromal cells enhances osteogenic activity, simulating the effect of magnesium alloy degradation, Acta Biomater10(6) (2014), 2834. doi:10.1016/j.actbio.2014.02.002.

22.

Lavenus

Berreur

Trichet

Pilet

Louarn

and Layrolle

, Adhesion and osteogenic differentiation of human mesenchymal stem cells on titanium nanopores, Eur Cell Mater22 (2011), 84. doi:10.22203/eCM.v022a07.