Abstract
Summary
Cells cultured in vitro from porcine kidneys support growth of JE virus and are concomitantly destroyed. Each of 5 virus strains (from pig, bird, mosquito and man) was propagated serially through 5–28 passages. Virus harvests from porcine kidney cultures titrated 105–8 ID50/ml in mice and in PK cultures. Total destruction of cells by recently isolated viruses in low mouse passage occurred usually within 2–5 days after inoculation of cultures, and could be prevented by specific antiserum.
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