Immunochemical Studies with Radioactive Isotope,Similarity and Difference Between Serum Vitellin and Lipovitellin

Abstract

Summary

With the help of radioisotope, immunochemical studies on the similarity and differences between serum vitellin and yolk lipovitellin, were made. When P³²-containing serum of laying hens was used as antigen, each antiserum to the serum of laying hens (absorbed with cock serum and unabsorbed, to yolk, absorbed and unabsorbed, and to lipovitellin, absorbed and unabsorbed), precipitated 95% of the radioactivity of P³² in protein P fraction, and 50-65% in lipid P fraction. On the other hand, when P³²-containing yolk solution in 10% NaCl was used as antigen, these antisera precipitated only 22-36% of radioactivity in the former fraction, and 55-80% in the latter fraction. The ratio of lipid P to protein P in the precipitates obtained with the sera of laying hens and the above described antisera was 1.1-1.6:1, and, in those obtained with yolk and the antisera was 4.5-8:1, compared with 1.6:1 for the lipovitellin used. Injection of the serum of laying hens, yolk and lipovitellin into rabbits produced antibodies which precipitated a similar lipoprotein.