Abstract
Summary
The in vitro formation of fibrous membranes by the L and LLC-Ml strains of mouse fibroblasts in a protein-free medium has been demonstrated. When cells of either of these strains are suspended in medium 199 plus 0.5-1.0% peptone and incubated at 35°C on a rotary action shaker at 100 rpm, delicate membranes form at the liquid-air interface. Enzymatic digestion, staining reactions and electron micrographs suggest that the fibers are fibrous protein in nature and belong to the collagen class of protein. The possible relationship of this fibrous protein to the cell surface is discussed.
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