Incorporation of C 14 of Acetate-1-C 14 and Pyruvate-2-C 14 into Brain Cholesterol in the Intact Rat

Abstract

Summary

Incorporation of C¹⁴ into brain cholesterol following the intracisternal injection of acetate-1-C¹⁴ and pyruvate-2-C¹⁴ has been investigated. Incorporation was observed in both young and adult rats, but the amount of incorporation was much greater in the younger animals. The incorporation with pyruvate was less than that with acetate. Simultaneous injection of pyruvate-2-C¹⁴ and unlabeled acetate resulted in reduced incorporation of the C¹⁴ of the pyruvate. No decline in activity of cholesterol was observed during a 72 day period following the labeling of the compound by injecting labeled acetate.