Studies on Propagation of the Col SK Group of Viruses in Various Tissue Culture Media. ∗

Abstract

Summary and conclusions

Results of a comparative study of the cell responses induced by several strains of the Col SK group of viruses in various tissue culture substrates are reported. L cells, HeLa cells and KB cells supported cytopathogenic growth of EMC and of Mengo virus, F virus grew only on L cells, and Col SK and MM viruses failed to grow in any of the 3 substrates. HeLa tissue cultures inoculated with EMC virus, in remote serial passage, were infectious for HeLa cells and for mice at an ID₅₀ of about 10⁵ per 1 cc of culture fluid and had a hemagglutinating titer of between 1:2 and 1:4. Data permitting the establishment of a growth curve of EMC tissue culture virus on HeLa cells are submitted.