Abstract
Summary
An attempt was made by labeling plasma proteins with the blue dye T-1824 to estimate the amount of protein filtered and reabsorbed by the kidney under several conditions. A single intravenous injection of 2.5 mg of T-1824 into normal rats did not cause the urine to be blue and the kidneys examined 24 hours after the injection exhibited only faint blue staining of the proximal convoluted tubules of the cortex. When proteinuria was produced by a single small injection of uranyl acetate, the injection of T-1824 was followed by blue staining of the urine. However, the cortices of the kidneys contained less blue dye than those of control animals, indicating, presumably, that there was less protein reabsorption than normal by the damaged tubular cells. Injection of the blue dye into nephrotic rats with proteinuria due to administration of nephrotoxic anti-rat kidney serum was followed by blue staining of the urine and intense blue staining of the cortices of the kidneys. This presumably indicated that protein reabsorption by the tubular cells was greater than normal, and that the proteinuria was a result of increased glomerular permeability and not of decreased tubular reabsorption of normally filtered protein.
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