Role of Various Tissues in Metabolism of C 14 Labeled Priscoline in the Rat. ∗

Abstract

Summary

Further studies on in vivo and in vitro metabolism of Priscoline are reported. Analyses of tissues, small intestinal contents, plasma, and urine of rats given radioactive Priscoline hydrochloride intravenously showed that almost all of the C¹⁴ was recovered as unchanged Priscoline both 1 and 2 hours after injection. Liver, small intestinal contents, and urine contained small but significantly increasing percentages of a 50% methanol soluble radioactive metabolite. About 27% of the C¹⁴ in liver was found as this fraction after 2 hours. When rat tissue slices were incubated with radioactive Priscoline under oxygen, liver and kidney took up the highest concentrations of C¹⁴. Liver slices metabolized small amounts of the drug to the 50% methanol soluble fraction under aerobic conditions, but not under anaerobic conditions. There appeared to be no correlation between Qo₂ values of tissues and their particular role in the disposal of Priscoline.