Occurrence in Plasma of an Extractable Lipid Mobilizer (LM) ∗

Abstract

Summary

1. Freeze dried dialyzates of plasma prepared from dogs, rats and horses receiving cortisone contained a lipid mobilizer (LM) which markedly elevated plasma levels of free and esterifled cholesterol, lipid P and total fatty acids when administered to fasted mice, rats, rabbits, guinea pigs, dogs and humans by intramuscular or intravenous injection. LM was not active by the oral route. The dialyzate also inhibited in vitro the delactescing action of heparin clearing factor. Plasma dialyzates prepared from untreated humans, horses, dogs and rats were 1/1000 as potent. 2. Cortisone injection failed to release LM in hypophysectomized rats. 3. LM activity of the dialyzate is not due to the same constituent responsible for CFI activity. 4. Hyperlipemia resulting from a single large dose persisted for at least one week in rabbits and dogs. 5. Repeated large doses of LM resulted in sustained and progressive hyperlipemia, hyperphagia and either loss in body weight or failure to gain. 6. Marked depletion of perirenal, mesenteric, omental, spermatic cord and subcutaneous fat were observed in dogs receiving weekly injections of LM.