Abstract
Summary
The biosynthesis of the C14 labeled benzylpenicillin was achieved by preparing phenylacetic acid-l-C14. and incorporating the latter into the side-chain of benzyl-penicillin. The antibiotic was successfully separated from the nonreacted labeled phenylacetic acid by column chromatography. The data obtained by radioautography and chemical degradation of the purified antibiotic indicated that the radioactivity resided in the side-chain of the benzylpenicillin molecule.
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