Abstract
Summary
A method is described for estimating catalase activity by the amount of heat liberated. The latter is estimated from the temperature rise of the solution, the maximum temperature being conveniently recorded by a clinical thermometer. The average temperature rise of the solution to which 0.01 ml of human blood was added was found to be about 12°C, corresponding to a liberation of 0.4 M or 14 g of H2O2 by each ml of blood. Some comparisons are made of the time course of the liberation of heat with that of the evolution of O2 and the disappearance of H2O2 as estimated by titration.
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