Tissue Glycogen Storage as Affected by Acetoacetate

Abstract

Summary

1. Glycogen contents of rat liver and leg muscle were unchanged after daily injection of acetoacetate and propionate in increasing amounts for 21 weeks. 2. Ability of very small amounts of insulin to promote glycogenesis in surviving hemidiaphragms was not inhibited by 50 mg % of acetoacetate. 3. A rapid hydrolysis of the glycogen of liver slices was unaffected by 125 mg % of acetoacetate in the buffer in the absence of glucose, but glycogenolysis was markedly decreased if both were present. 4. Acetoacetate in these concentrations does not appear to inactivate insulin, to inhibit glycogen storage in the muscle or liver tissue, or to affect appreciably the utilization of carbohydrate as measured by the glucose tolerance.