Abstract
Summary
1. The oxycellulose technic for isolation of ACTH from pituitary has been successfully employed to concentrate and quantitatively to measure blood ACTH. The method a) appears to be specific for ACTH since hypophysectomized rat blood induces no adrenal ascorbic acid depletion in the assay animal, b) almost completely recovers ACTH added to hypophysectomized rat blood, c) almost completely recovers endogenous ACTH, d) yields concentrates which are not toxic to the assay animal and e) is reproducible. 2. It is estimated that rat blood, obtained by acute exsanguination of intact animals, contains 2 to 3 mu ACTH per 100 ml, whereas blood obtained under similar conditions from rats bilaterally adrenalectomized for one week contains 10 to 12 mu ACTH per 100 ml.
Get full access to this article
View all access options for this article.