Effect of Vitamin E and CCL 4 on Fat,Respiration and Choline Oxidase of Rat Livers. ∗

Abstract

Summary

(1) Liver fat of young rats on a vit. E-deficient, 10% casein diet was increased 4 percentage points, from 8.7 to 12.7, by the inclusion of alpha tocopheryl acetate in the diet, a highly significant difference statistically. When the diet contained 18% casein, no difference was noted due to supplements of alpha tocopheryl acetate. (2) Dietary treatments did not influence the increase in liver fat 24 hours after CC₁₄ injections, though vit. B₁₂ supplements resulted in more rapid return to normal values. The rate of respiration of liver slice in glucose-Ringers was doubled following CC₁₄ injections. This was substantially inhibited by dietary supplements of vit. E. (3) Choline oxidase activity of the liver was not influenced by dietary vit. E or by CC₁₄ injections into rats, although increased dietary fat level increased the activity of this enzyme system.