Abstract
Summary
Frozen-dried, dry mounted Feulgen stained preparations of irradiated rat and mouse tissues were employed to study early cytologic changes after ionizing radiation. Fine nuclear details are preserved by the freezing-drying method. Lethal radiation effects observed in radiosensitive cells include formation of large intranuclear vacuoles with stretching of nuclear membranes. Nonlethal changes in radioresistant tissues comprise smaller intranuclear vacuoles, decreased Feulgen stain of nuclear chromatin and sap, and loss of regular sharp staining of nuclear membranes. Such changes in liver cells may contribute to the acute radiation syndrome. Histochemical technics may provide a better understanding of tissue changes after ionizing radiation.
Get full access to this article
View all access options for this article.