Abstract
In the past 2 years much has been written seemed to us of interest to discover whether concerning the effect of Aminopterin on normal and leukemic cells( 1 ) The remissions which occur in acute leukemia, especially acute lymphoid leukemia in childhood, are now well known. Because of this wok it seemed to us of interest to discover whether this substance could in any way alter the pathologic changes we have obtained with myelokentric and lymphokentric acids( 2 , 3 ).
Material and methods. Guinea pigs were given daily intramuscular injections of crude myelokentric or crude lymphokentric acid. O.S ml to 1.0 ml of urinary extract was given daily in aqueous suspension at a pH of 7.8 for 9 to 14 days. The total dose of extract given was made from 2 to 6 liters of urine. Ten guinea pigs, as well as receiving crude myelokentric acid, also were given varying amounts of Aminopterin, the daily dose being 2 mg; and as controls 10 animals were given 10 mg daily of Teropterin as well as crude myelokentric acid. Crude lymphokentric acid was given to 6 animals; to 4 of them 2 mg daily doses of Aminopterin were given, and the other two were given 10 mg daily doses of Teropterin. Four animals were given 2 mg daily doses of Aminopterin only.
Results. The results are summarized in the table, in which it will be seen that animals that received crude myelokentric acid are designated under result as being positive for extra medullary myelopoiesis, + to +++ M; or if negative for this finding, −M; and equivocal animals are marked ±. Liver, spleen and adrenals were examined for extra medullary myelopoiesis and are graded − to +++ under such headings.
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