Hemolysis of Erythrocytes Incubated in Salines

Abstract

Summary

In 1:100 dilutions of dog blood with buffered salines ranging from 0.41% to 1.28% the hemolysis resulting from incubation for 22 hours or less at 37°, 42° and 45°C varied directly although not proportionately with the osmotic tonicity of the saline.

Ninety-five percent or more of the expected hemolysis resulting from incubation in 0.70% saline at 37°C could be prevented by the addition of 32 mg of dextrose to 100 ml of the 1:100 saline dilution of blood, and as little as 1 mg of dextrose per 100 ml appreciably reduced 22 hour incubation hemolysis.