Abstract
Summary
The method described in this paper incorporates the following desirable features:
1. It is possible to prepare radioautographs from tissue that has been frozen and dehydrated in vacuum and mounted by procedures that avoid contact of the tissue with reagents that would extract the radioactive material.
2. Sharp images are obtained; yet the method avoids possible difficulties due to direct chemical contact of the tissue with the photographic emulsion.
3. There are no difficulties in staining and developing, since the two slides are handled separately.
4. Due to the fact that two images are on separate slides, the photographic image formed does not obscure structural details of the tissue section or vice versa; comparisons are always made on the same tissue section that was used in making the photographic exposure.
5. It is possible to match localizations accurately on the two slides.
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