Effects of Use and Disuse on Nerve Endings,Neurosomes,and Fiber Types in Skeletal Muscle. ∗

Abstract

Summary

The limited experimental evidence presented in this paper tends to support the following statements:

The normally used and innervated gastrocnemius muscle of the white rat is characterized histologically by dark, coarsely granular and light, finely granular and agranular muscle fibers. The nerve endings are usually retracted in the dark, coarsely granular fiber and expanded in the light, agranular fiber, as revealed by gold impregnation of teased whole muscle fibers. The atrophy of disuse following tenotomy of the gastrocnemius muscle with nerve supply intact, is accompanied, during the first month, by the progressive loss of the narrow and dark, coarsely granular muscle fiber, and by a depletion of its innervation. The dark, granular muscle fiber is determined by the reciprocal interaction of the normally intact and attached muscle fiber with its normally functioning innervation. During the process of atrophy of disuse after tenotomy, small and giant fusiform neurosomes are discharged from the altered nerve endings. It is assumed that these giant fusiform neurosomes are the product of a retardation in the rate of the discharge, diffusion, and disappearance by hydrolysis, following tenotomy and disuse atrophy of the innervated gastrocnemius muscle. There appears to be a parallelism between the atrophy by disuse of tenotomized muscle and the loss of the normal discharge of neurosomes from the altered and progressively depleted innervation of the muscle. One factor in the atrophy of disuse of muscle appeared, therefore, to be the substantial loss of the discharge of neurosomes into muscle as well as the quantitative decrease of the myoplasm. The giant fusiform neurosomes that appear during the early period following tenotomy disappear when the living muscle in situ is adequately restretched prior to excision and gold impregnation.