Abstract
Summary
1. Details are given for a method of inactivating streptomycin with semicarbazide-hydrochloride in order to test the sterility of concentrated streptomycin solutions. It has been shown that although several carbonyl reagents inhibit bacterial growth, it requires from 533 to >11,000 times more semicarbazide-hydrochloride (one of the least toxic of the group) to cause this inhibition than is required of streptomycin.
2. Spores of Bacillus sp. No. 290, when added to a streptomycin solution containing 28,700 units per ml, were able to grow out when diluted in thioglycolate broth. When a similar spore suspension-streptomycin mixture was treated with semicarbazide-hydrochloride (3, 2 or 1 y carbonyl reagent per unit (or y) of streptomycin) and then diluted in thioglycolate broth, growth occurred at a dilution.
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