Abstract
Summary
1. Agar slant tissue cultures as old as 17 days will permit the survival of the inoculated herpes simplex virus for at least 96 hr. 2. There appears to be a rough inverse relation between the age of the inoculated tissue culture and its virus content 96 hr later. 3. The virus may be propagated indefinitely on fresh agar slant tissue cultures; such is not the case if cultures 4 days or older are employed as the media for transfer.
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