Carbohydrate Metabolism of Oidium lactis and Bacillus subtilis in Complex Carbohydrate-rich Culture Medium. ∗

Abstract

Summary

Oidium lactis and B. subtilis were inoculated into culture media prepared from 0.3% solution of meat extract or an infusion of beef muscle, to which were added 1% of Witte peptone, 1 to 1.8% of Na₂HPO₄ · 12 H₂O and 0.9% of dextrose. Both microorganisms grew rapidly at 37.5° and both yielded about 70% of iactic acid on the basis of the sugar consumed. Approximately one-fifth of the C₃-intermediates from the sugar was further metabolized into 2 moles of formic acid and 1 mole each of acetic acid and ethyl alcohol. Their metabolism in the same culture medium is compared with that of Eberthella typhosa, Shigella paradysenteriae, Lacto-bacillus helveticus (casei eP) and Corynebacterium diphtheriae.