Abstract
In a series of 100 determinations of blood iodine by the McClendon-Bratton method it appeared as if a long time was required to eliminate a dose of Lugol's solution from the blood.
This suggested the desirability of developing a method for fractionating quantitatively the iodine of blood. We found when a sample of blood is divided into 2 parts and to 1 part KI is added and then the blood samples are subjected to the following procedure that the KI does not appreciably raise the blood iodine.
Five cc of blood is spurted through a fine opening into 100 cc of methanol in a glass-stoppered flask of capacity of about 115 cc to the stopper. This is violently shaken and then allowed to settle. The methanol is decanted and 100 cc of acetone introduced and the shaking repeated. An 8-inch length of 3/8-inch Visking sausage casing is closed at one end by any method, and the other end tied onto a 100 cc burette without stop-cock. The acetone suspension is shaken and poured into the burette. The acetone filters through the casing and the blood residue remains in the casing. After the visible liquid has evaporated, the casing is suspended in air and dried 24 hours or in an oven at 100° and dried for one hour and then analyzed by the McClendon-Bratton method. 1
It was shown that although KI is washed out of the blood by this means that thyroglobulin-iodine added to the blood is entirely retained. Therefore the method is provisionally considered a method of determining “hormone iodine.”
The values of 125 determinations on human blood obtained by this method are near 0.3 γ in 5 cc whereas the normal of total blood iodine is near 0.5 γ in 5 cc. Taking 2.5 g iodide by mouth did not greatly raise the “hormone iodine.”
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