Inhibition of Brown-Pearce Rabbit Tumor with Filtered Homologous Tumor Material

Abstract

In the course of some experiments with the Brown-Pearce rabbit tumor 1 , 2 , 3 an unusual inhibitory effect was noted following a single injection of 0.3 cc. of a Berkefeld “V” filtrate of fresh tumor given 2 weeks before the tumor inoculation. The experiment had not previously been tried and has not yet been repeated. It would not be reported here except that it is the only case of complete failure of the Brown-Pearce rabbit tumor to grow following intratesticular inoculation in some 7 years of experience involving approximately 100 different transplantations of the tumor, and approximately 1000 rabbits. This experience includes studies on some 20 pure breeds, and on various types of hybrids, particularly with the type of brown-gray hybrids used in the experiment; and the probability of a rabbit being free of tumor at the end of a 2 months'observation period was calculated to be 0.2306. The occurrence of all 6 animals in an experiment being negative by accident is, therefore, very unlikely (P = 0.01—).

Furthermore the 6 animals of this unusual group were identical as to source, age, weight, coat color, vigor, size of testicles, and body build with 36 other rabbits not receiving the filtrate and composing 6 additional groups inoculated on the same day in staggered order 3 in the same testicle with the same dosage of the same fresh tumor. One of the 6 additional groups was a control group and was not otherwise treated. Analyzed per animal inoculated, the incidence of primary tumors in this group was 50% and the tumors averaged 5.8 cc.; the incidence of metastatic tumors was 83.3%; the metastatic tumor averaged 53.0 cc. in volume per animal and was distributed over 6.3 metastatic foci; the mortality was 61.1% over a period of 59 days. The 5 additional groups were treated with various other homologous materials before tumor inoculation and had an incidence of primary tumors of 40% which averaged 3.8 cc.; the incidence of metastatic tumors varied from 80 to 100% per group, and averaged 88.0%; the volume of metastatic tumor per group varied from 40 to 162 cc. per animal and averaged 94 cc.; the number of metastatic foci varied from 10.2 to 24.2 per group and averaged 13.9 foci; the mortality varied from 60 to 100% and averaged 71.4%; the longevity varied from 48 to 61 days and averaged 54.7 days.