Failure of a Mouse Carcinoma Material to Enhance a Mouse Sarcoma

Abstract

It has been shown that an homologous rabbit carcinoma enhancing material 1 , 2 failed to enhance a carcinoma of the mouse. 3 Haaland, 4 Leitch, 5 and Casey 5 have demonstrated, however, that a material can be obtained from the same mouse carcinoma (No. 63) which will enhance both the incidence and the growth of the primary tumor when used in conjunction with the tumor inoculation. It seemed desirable, therefore, to determine whether homologous enhancing materials will enhance heterologous transplanted tumors within the same animal species. Since other transplantable rabbit tumors are not available, the experiments were carried out with mouse tumors. In the 2 experiments here reported the enhancing material was obtained from the Bashford mouse carcinoma (No. 63) and its effect was observed in connection with implants of mouse sarcoma (No. 180). Two different batches of preserved (enhancing) material and sarcoma emulsion were used; 60 mice of the Rockefeller Institute strain were employed; in the first experiment, there were 40 females and in the second 20 males. Thirty of these mice were injected with a saline emulsion of Bashford mouse carcinoma (No. 63) tissue which had been preserved anaerobically at 24°F. for 42 days. 3 Two weeks later the entire 60 mice were inoculated into the left groin with 0.09 cc. of an emulsion (10 parts saline, 1 part tumor) of fresh mouse sarcoma (No. 180). Measurements of the local growths were made at 6, 12, and 16 days. The ulceration of many tumors after this time precluded further measurements.

For the sake of clarity the mice treated with the preserved carcinoma material and later inoculated with the sarcoma are called the “experimental” mice, and the mice inoculated only with the sarcoma the “control” mice.